Morphine Actions on the Immune System

吗啡对免疫系统的作用

• 批准号: 7496655
• 负责人: SULIE L. CHANG
• 金额: $ 12.02万
• 依托单位: SETON HALL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-02-05 至 2013-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7496655
• 关键词: Adhesions; Adrenal Glands; Affect; Affinity; Animals; Anti-Inflammatory Agents; Arts; Attenuated; Award; Bacterial Infections; Basic Science; Biological Assay; Blood Coagulation Factor; Blood capillaries; Brain; Canis familiaris; Cell Nucleus; Cell model; Cells; Characteristics; Chemosensitization; Chronic; Clinical; Communities; Condition; Corticosterone; Data; Disease; Disseminated Intravascular Coagulation; Drug abuse; Endorphins; Endothelial Cells; Endotoxins; Exposure to; Fibrinopeptide A; Fluorescence; Fluorescence Microscopy; Funding; G-Protein-Coupled Receptors; GTP-Binding Proteins; Grant; HIV; HIV Infections; HIV-1; Housing; Hypothalamic structure; Immune; Immune response; Immune system; In Vitro; Independent Scientist Award; Infection; Inflammation; Inflammatory; Inflammatory Response; Institutes; Interleukin-1; Interleukin-1 Receptors; Interleukin-6; Interleukins; Kidney; Leukocytes; Ligand Binding; Lipopolysaccharides; MDCK cell; Mediating; Mentors; Mesentery; Messenger RNA; Microarray Analysis; Mission; Molecular; Morphine; Mus; NF-kappa B; National Institute of Drug Abuse; Neurons; Neurosecretory Systems; Opiates; Opioid; Opioid Peptide; Opioid Receptor; Pathway interactions; Pharmacology; Pituitary Gland; Plasma; Procedures; Process; Production; Rattus; Reporting; Research; Research Design; Research Personnel; Research Project Grants; Reverse Transcriptase Polymerase Chain Reaction; Science; Scientist; Shock; Signal Transduction Pathway; Students; System; Testing; Time; Training; Training Programs; Translating; Transmembrane Domain; Tumor Necrosis Factor-alpha; Universities; Work; antithrombin III-protease complex; base; behavior test; capillary; career; college; cytokine; drug of abuse; endogenous opioids; experience; hypothalamic-pituitary-adrenal axis; in vivo; instrumentation; mu opioid receptors; paraventricular nucleus; pressure; protein expression; receptor; receptor expression; research and development; response; venule

DESCRIPTION (provided by applicant): This is a renewal application for my K02 Independent Scientist Award that will enable me to continuously obtain maximum protected time at Seton Hall University (SHU) for working on my NIDA funded projects and mentoring research students and junior scientists. During the current K02 funding period, I have worked on and successfully renewed my long-standing R01 grant entitled, "Morphine Actions on the Immune System", and have expanded my research projects as evidenced by receiving an R21 grant, "Opiate's Effects on HIV- 1 Induced Inflammation in the CMS", and an R03 grant, "Effects of Morphine on HIV-1 Neuroinvasion and Brain Changes in Mice" (Co-Pi with Dr. David J. Volsky), to study the interactions between drug abuse and HIV infection. I have implemented a confocal facility at SHU via my NSF Major Research Instrumentation grant, and have gained extensive experience in real time RT-PCR, fluorescence-based biological assays, and microarray technology. I have mentored research students and junior researchers, established an ongoing training program in the responsible conduct of research (RCR), and created a semester-wide Molecular Bioscience Meeting to enable graduate and undergraduate students to interact scientifically. In addition, I have helped establish the Institute of Neurolmmune Pharmacology (I-NIP), one of its missions is to translate bench work to the community. With this K02 grant renewal, I will: 1) Work on my R01 DA007058-17-22 (PI), and extend the studies proposed in my R21 DA019836-01-02 (PI) and R03 DA022169-01-02 (Co-Pi) awards to develop a new R01 project, respectively; 2) Gain experience in using quantitative fluorescence microscopy, stereological procedures, and MicroBrightfield NeuroLeucida system in order to bring my in vivo research to the level of a single cell; 3) Gain experience in animal behavioral testing to determine the effects of drug abuse in the presence of HIV infection; 4) Bring basic science to the community by collaborating with clinical scientists and fulfilling the missions of the I-NIP; and 5) Continue to mentor research students, junior scientists, and under-represented students, emphasizing the RCR. My research projects and my initiatives in research-oriented training that are housed in the I-NIP are valuable assets to SHU, and the career development and research plans as proposed in this K02 renewal application have received tremendous support from the College of Arts and Sciences at Seton Hall University.

描述（由申请人提供）：这是我的K 02独立科学家奖的续期申请，这将使我能够在西顿霍尔大学（SHU）持续获得最大的保护时间，用于我的NIDA资助的项目和指导研究学生和初级科学家。在目前的K 02资助期间，我一直致力于并成功地更新了我长期以来的R 01资助，题为“吗啡对免疫系统的作用”，并扩大了我的研究项目，这一点可以通过接收R21资助，“阿片类药物对CMS中HIV- 1诱导炎症的影响”和R 03资助来证明。“吗啡对小鼠中HIV-1神经侵袭和大脑变化的影响”（Co-Pi与大卫J.沃尔斯基博士），研究药物滥用和HIV感染之间的相互作用。我已经通过我的NSF重大研究仪器资助在上海大学实施了共聚焦设施，并在真实的时间RT-PCR，荧光生物测定和微阵列技术方面获得了丰富的经验。我指导了研究生和初级研究人员，建立了一个持续的培训计划，在负责任的研究行为（RCR），并创建了一个学期的分子生物科学会议，使研究生和本科生科学互动。此外，我还帮助建立了神经免疫药理学研究所（I-NIP），其任务之一是将实验室工作转化为社区工作。通过此次K 02资助更新，我将：1）致力于我的R 01 DA 007058 -17-22（PI），并扩展我的R21 DA 019836 -01-02（PI）和R 03 DA 022169 -01-02（Co-Pi）资助中提出的研究，以分别开发新的R 01项目; 2）获得使用定量荧光显微镜，体视学程序和Microphilfield NeuroLeucida系统的经验，以便将我的体内研究带到单细胞水平; 3）在动物行为测试方面取得经验，以确定在艾滋病毒感染的情况下滥用药物的影响; 4）通过与临床科学家合作并履行I-NIP的使命，将基础科学带到社区; 5）继续指导研究生、初级科学家和代表性不足的学生，强调RCR。我的研究项目和我在研究型培训中的举措是在I-NIP中的宝贵资产，SHU，并且在此K 02更新申请中提出的职业发展和研究计划得到了Seton Hall University文理学院的巨大支持。