High Resolution fMRI of Nociception in SII of Monkeys

猴子 SII 伤害感受的高分辨率功能磁共振成像

• 批准号: 7532716
• 负责人: Li Min Chen
• 金额: $ 19.19万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7532716
• 关键词: Animals; Area; Brain; Characteristics; Computer information processing; Condition; Data; Depth; Digit structure; Distal; Electrodes; Electrophysiology (science); Flowcharts; Functional Imaging; Functional Magnetic Resonance Imaging; Heating; Human; Image; Individual; Knowledge; Lead; Location; Magnetic Resonance Imaging; Maps; Measures; Methods; Microelectrodes; Modality; Monkeys; Neurons; Nociception; Nociceptive Stimulus; Pain; Parietal; Pattern; Perception; Process; Property; Public Health; Resolution; Response to stimulus physiology; Rest; Role; Saimiri; Sensory; Sensory Process; Signal Transduction; Site; Somatosensory Cortex; Specificity; Stimulus; Structure; Sylvius fissure; Tactile; Temperature; Touch sensation; awake; base; millimeter; nociceptive response; nonhuman primate; novel therapeutics; receptive field; research study; response; size; vibration

DESCRIPTION (provided by applicant): This proposal aims to study the cortical representation of the sensory aspect of pain and touch within the traditionally defined secondary somatosensory cortex (SII) located along the Sylvian fissure in non-human primates. By using high resolution fMRI and image-guided electrophysiological recordings in anesthetized squirrel monkeys, we will examine functional roles of the subregions within SII in the processing of basic features of painful and tactile inputs. There are two specific aims. Aim 1 proposes to determine which subregion(s) within SII cortex is activated during painful and tactile stimulation of individual fingerpads; whether nociceptive and tactile responses colocalize within each activated subregion; whether there is a fine digit topography and stimulus intensity-dependent response for pain and/or touch; and what is the temporal property of the responses. FMRI activation maps to painful heat and vibration will be compared to evaluate the specificity of activation. Aim 2 proposes to determine the neuronal mechanism underlying the activations revealed by fMRI. FMRI activation maps will be validated and spatially correlated with the maps determined by electrophysiology. By using the fMRI activation maps as a guide, we will place single or multiple electrodes in the activated regions to characterize and record neuronal response properties in response to painful heat, vibration, and rest conditions, and to determine what aspect of neuronal activities (single, multiple neuron, or local field potential) correlates best with the stimulus-response function of the fMRI signal. This study will provide direct evidence relating the existence of nociceptive specific regions within SII cortex and the linkage between neuronal activities and fMRI signal. Ultimately, knowledge gained will have profound impact on our understanding of the cellular mechanism of pain processing and perception. 30 PUBLIC HEALTH RELEVANCE This study centers on understanding which cortical areas encode the location (where), intensity (how strong), and modality of a painful stimulus. If we can understand how these areas are organized and activated during sensory processing, and how they are connected to form a processing network, we will be able to understand the flowchart of information processing in the brain. This knowledge would promote new therapeutic ideas, which will lead to more rational and reliable treatment for pain.

描述（由申请人提供）：本提案旨在研究位于非人类灵长类动物大脑外侧裂沿着的传统定义的次级躯体感觉皮层（SII）内疼痛和触觉感觉方面的皮层表征。通过使用高分辨率的功能磁共振成像和图像引导的电生理记录在麻醉的松鼠猴，我们将研究的功能作用的SII内的子区域的疼痛和触觉输入的基本特征的处理。有两个具体目标。目的1提出确定SII皮质内的哪个子区域（S）在疼痛和触觉刺激的个人指垫期间被激活;伤害性和触觉反应是否在每个激活的子区域内共定位;是否存在精细的数字地形和刺激强度依赖性疼痛和/或触摸的反应;以及反应的时间特性是什么。将比较FMRI激活映射到疼痛的热和振动，以评估激活的特异性。目的二是探讨功能磁共振成像所揭示的激活的神经机制。将验证功能磁共振成像激活图，并将其与电生理学确定的图进行空间关联。通过使用fMRI激活图作为指导，我们将在激活区域放置单个或多个电极，以表征和记录神经元对疼痛的热、振动和休息条件的反应特性，并确定神经元活动的哪个方面（单个、多个神经元或局部场电位）与fMRI信号的刺激-反应函数最相关。本研究将为SII皮质内存在伤害性感受特异区域以及神经元活动与fMRI信号之间的联系提供直接证据。最终，获得的知识将对我们理解疼痛处理和感知的细胞机制产生深远的影响。30公共卫生相关性这项研究的中心是了解哪些皮层区域编码疼痛刺激的位置（在哪里），强度（有多强）和形式。如果我们能够理解这些区域在感觉处理过程中是如何组织和激活的，以及它们是如何连接形成处理网络的，我们将能够理解大脑中信息处理的流程图。这些知识将促进新的治疗理念，这将导致更合理和可靠的疼痛治疗。