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Resting State Connectivity in Primate Spinal Cord

灵长类动物脊髓的静息态连接

基本信息

批准号：
9221377
负责人：
Li Min Chen
金额：
$ 51.69万
依托单位：
VANDERBILT UNIVERSITY MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-02-15 至 2021-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9221377
关键词：
Address Adopted Anatomy Animals Architecture Autopsy Behavior Behavioral Biological Markers Biophysics Blood Vessels Brain Cervical spine Data Detection Disease Electrophysiology (science)Evaluation Exhibits Foundations Frequencies Histologic Histology Horns Human Injectable Injury Intervention Investigation Knowledge Magnetic Resonance Maintenance Maps Measurable Measurement Measures Methods Modeling Monkeys Motivation Nature Nerve Block Neurons Pathologic Patients Pattern Pharmacology Physiology Play Population Primates Recovery Reporting Reproducibility Research Resolution Rest Retrieval Role Saimiri Seeds Signal Transduction Site Somatosensory Cortex Spinal Spinal Cord Spinal Cord Diseases Spinal Injuries Spinal cord grey matter structure Spinal cord injury Stimulus Structure Surface Time Treatment Efficacy Variant Vertebral column anatomical tracing base blood oxygen level dependent gray matter high resolution imaging human subject imaging biomarker independent component analysis insight neural circuit nonhuman primate novel strategies programs public health relevance relating to nervous system sensory input

项目摘要

DESCRIPTION (provided by applicant): This proposal aims to detect and characterize inter-regional correlations in resting-state fluctuations of functional magnetic resonance (fMRI) signals within the spinal cord (SC), and to validate their neuronal and anatomical bases as measures of functional connectivity. Our discovery of synchronized variations of MRI signals between the various horns of spinal cord grey matter in humans and monkeys suggest these patterns depict neural circuits of functional significance. Moreover, their changes post-injury suggest they may provide practical imaging biomarkers of functional integrity. Our earlier studies in human subjects have stimulated this parallel program of research using non-human primates (NHPs). We will use very high-resolution imaging at high field (9.4T) to study networks in the grey matter of the spines of anesthetized monkeys, extending our previous studies of the functional organization of primary somatosensory cortex. Although there have been several 1000s of studies reported that have used resting state fMRI to detect and characterize functional connectivity in the brain, to date there have been only a handful of studies of task-induced activation, and no previous reliable findings of resting state fluctuations, in the grey matter of the SC. Our preliminary studies in humans and NHPs have adduced strong evidence that resting state variations are reliably measurable, reproducible, and produce patterns depicting distinct neural circuits within and across spinal segments. However, although resting state correlations in brain are already being widely exploited, their precise interpretation remain unclear, and their biophysical basis as indicators of functional connectivity is unsubstantiated. This is even more the case in the spine, where we have much less knowledge of the detailed vascular physiology or organization of specific functional neural circuits. We therefore propose [1] to characterize the spatial connectivity patterns of resting state fMRI signals at sub-millimetr resolution in the spinal cords of NHPs; [2] validate the connectivity measures from resting state fMRI signals by comparisons with quantitative electrophysiology and histology; and [3] validate the interpretation of connectivity measures from resting state MRI signals by comparisons before and after pharmacological manipulations and spinal cord injury. We will acquire resting state fMRI data, perform electrophysiological measurements, evaluate behavior and quantify connectivity from post-mortem histology in monkeys before and after unilateral transectional injuries to the spine, and after pharmacological modulation of afferent inputs. Overall, this research would establish new insights into the functional architecture of the SC and provide new opportunities to investigate SC function in normal and pathological conditions. It would provide a firm foundation for the application of resting state MRI to assess the functional integrity and recovery post-injury of the spine in patients suffering a variety of disorders of the SC.