Recovery of Bluetongue Virus from nucleic acid: configuration optimisation and application

核酸回收蓝舌病毒：构型优化及应用

• 批准号: BB/F007159/1
• 负责人: Polly Roy
• 金额: $ 75.54万
• 依托单位: London School of Hygiene & Tropical Medicine
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2008
• 资助国家: 英国
• 起止时间: 2008 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FF007159%2F1
• 关键词: Recovery; Bluetongue; Virus; nucleic; acid

Bluetongue is a viral disease of sheep (sometimes cattle and goats) that poses an economic threat to UK agriculture. Since 1998, outbreaks of Bluetongue have spread Northwards in Europe and last year the disease reached Holland. The problem of BTV is clearly growing and the use of modern genetic methods to improve vaccines as well as to understand every aspect of the replication cycle is required. A significant bottleneck however, is the inability to recover BTV from sequence defined clones. As a result, the ability to change or delete essential genes in the virus with a view to producing attenuated strains as candidate vaccines is currently impossible. This project seeks to rectify this and produce a 'reverse genetics' system for BTV. Within the last year the research team has discovered a way to recover Bluetongue virus starting from just the viral genetic material isolated from the virus. The proposed research builds on this success to test ways in which the virus can be constructed completely from cloned gene copies and then deliberately weakened to make better vaccines. The research will specifically target the strain of virus that was the cause of last year's outbreak in Holland to make new strains that can be used to protect UK livestock against this disease. If successful it may be possible to also apply the research to make better vaccines for other viruses which cause disease in human and horses.

蓝舌病是绵羊（有时是牛和山羊）的病毒性疾病，对英国农业构成经济威胁。自1998年以来，蓝舌病的爆发已经在欧洲向北蔓延，去年这种疾病到达了荷兰。BTV的问题显然越来越严重，需要使用现代遗传学方法来改进疫苗以及了解复制周期的各个方面。然而，一个显著的瓶颈是不能从序列限定的克隆中回收BTV。因此，目前不可能改变或删除病毒中的必需基因以产生作为候选疫苗的减毒株。该项目旨在纠正这一点，并为BTV生产一种“反向遗传学”系统。在过去的一年里，研究小组发现了一种从病毒中分离出的病毒遗传物质中恢复蓝舌病病毒的方法。拟议中的研究建立在这一成功的基础上，以测试完全从克隆基因拷贝构建病毒的方法，然后故意削弱以制造更好的疫苗。这项研究将专门针对去年在荷兰爆发的病毒株，以制造可用于保护英国牲畜免受这种疾病侵害的新菌株。如果成功的话，这项研究也有可能应用于制造更好的疫苗，用于其他导致人类和马匹疾病的病毒。

项目成果

Bluetongue virus non-structural protein 1 is a positive regulator of viral protein synthesis.

• DOI: 10.1186/1743-422x-9-178
• 发表时间: 2012-08-29
• 期刊: Virology journal
• 影响因子: 4.8
• 作者: Boyce M;Celma CC;Roy P
• 通讯作者: Roy P