Realising the potential of a genetically engineered and uniquely cross protective vaccine for Bluetongue disease

实现基因工程和独特交叉保护蓝舌病疫苗的潜力

• 批准号: BB/J021342/1
• 负责人: Polly Roy
• 金额: $ 17.52万
• 依托单位: London School of Hygiene & Tropical Medicine
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2012
• 资助国家: 英国
• 起止时间: 2012 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FJ021342%2F1
• 关键词: Realising; potential; genetically; engineered; uniquely

Bluetongue disease is a debilitating and, in severe cases, lethal, animal disease that is a major economic threat to livestock and agriculture. Current vaccines available for Bluetongue are not considered to be safe enough for widespread use. Our laboratory has developed a replication defective (DISC) virus that can be used safely as an alternative BTV vaccine with none of the existing issues. In order to commercialize this vaccine a number of optimizations are necessary. In particular, the stability of the specialized cells which support the growth of the vaccine virus will be optimized. Moreover, to ensure that the DISC virus does not change with time, we will monitor for potential changes in the growth of the virus, expression of virus proteins and genome. The optimal storage conditions for vaccine and number of dose required will be determined in sheep to ensure that protection afforded is maximized.

蓝舌病是一种使人衰弱的动物疾病，严重时可致人死亡，对牲畜和农业构成重大经济威胁。目前可用的蓝舌病疫苗被认为不够安全，不能广泛使用。我们的实验室已经开发出一种复制缺陷病毒（DISC），可以安全地用作BTV疫苗的替代疫苗，不存在任何现有的问题。为了使这种疫苗商业化，有必要进行一些优化。特别是，支持疫苗病毒生长的特化细胞的稳定性将得到优化。此外，为了确保DISC病毒不随时间变化，我们将监测病毒生长、病毒蛋白表达和基因组的潜在变化。将在绵羊中确定疫苗的最佳储存条件和所需剂量，以确保最大限度地提供保护。

项目成果

Replication-Deficient Particles: New Insights into the Next Generation of Bluetongue Virus Vaccines.

• DOI: 10.1128/jvi.01892-16
• 发表时间: 2017-01-01
• 期刊: Journal of virology
• 影响因子: 5.4
• 作者: Celma CC;Stewart M;Wernike K;Eschbaumer M;Gonzalez-Molleda L;Breard E;Schulz C;Hoffmann B;Haegeman A;De Clercq K;Zientara S;van Rijn PA;Beer M;Roy P
• 通讯作者: Roy P

Generation of infectious RNA complexes in Orbiviruses: RNA-RNA interactions of genomic segments.

• DOI: 10.18632/oncotarget.12496
• 发表时间: 2016-11-08
• 期刊: Oncotarget
• 作者: Fajardo T Jr;AlShaikhahmed K;Roy P
• 通讯作者: Roy P

Highly efficient vaccines for Bluetongue virus and a related Orbivirus based on reverse genetics.

基于反向遗传学的蓝舌病毒和相关环状病毒的高效疫苗。

• DOI: 10.1016/j.coviro.2020.05.003
• 发表时间: 2020
• 期刊: Current opinion in virology
• 影响因子: 5.9
• 作者: Roy P

Pathogenicity study in sheep using reverse-genetics-based reassortant bluetongue viruses.

• DOI: 10.1016/j.vetmic.2014.09.012
• 发表时间: 2014-11-07
• 期刊: VETERINARY MICROBIOLOGY
• 影响因子: 3.3
• 作者: Celma, Cristina C.;Bhattacharya, Bishnupriya;Eschbaumer, Michael;Wernike, Kerstin;Beer, Martin;Roy, Polly
• 通讯作者: Roy, Polly