A Versatile High Resolution Low Vacuum FEG SEM for the Biosciences and Interdisciplinary Research

适用于生物科学和跨学科研究的多功能高分辨率低真空 FEG SEM

• 批准号: BB/F011105/1
• 负责人: E Ingham
• 金额: $ 29.58万
• 依托单位: University of Leeds
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2008
• 资助国家: 英国
• 起止时间: 2008 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FF011105%2F1
• 关键词: Versatile; Resolution; Low; Vacuum; FEG

Field emission gun scanning electron microscopy (FEG-SEM) is a type of microscopy capable of producing very high resolution images of the surface of a sample. It has a wide range of applications in biological and materials science in which researchers wish to visualise and analyse the surface of a sample over a wide range of magnifications. Field emmision gun scanning electron microscopy can be used to image over a large surface area, can be used to image bulk materials as well as thin films or spots and modern microscopes can image structures as small as one or two nanometres. Conventional FEG-SEM requires samples to be imaged under a high vacuum which means that specimens, for example biological materials which are wet, would produce a lot of vapour which interferes with the images. To visualise biological specimens by conventional FEG-SEM the specimens have to be dried and coated, which can distort images of structures. Another form of SEM, called environmental SEM (ESEM) allows samples to be visualised in low pressure gaseous environments and high humidity which means that biological samples can be imaged in their hydrated state either directly or in the frozen state. In this application we are seeking to replace a conventional SEM which is 27 years old, still requires film processing (does not acquire digital images) and frequently breaks down. We wish to purchase a versatile high resolution low-vacuum FEG-SEM and a cryo-workstation. The microscope is critically required for a large number of current and future projects in biological sciences, in particular research in tissue engineering, biomaterials, structural molecular and cellular biology. The microscope requested, is the most versatile high resolution feild emmision gun electron microscope available with extended low-vacuum capabilities. A major feature is that it does with a single tool, what used to require multiple systems. The scanning electron microscope has three modes of operation: high vacuum, low vacuum and environmental scanning (ESEM). The resolution achievable under different modes is 1-nanometres. The equipment will directly replace the old SEM in the current electron microscope unit in the Faculty of Biological Sciences. No refurbishment will be required. The use of the equipment will be supported by a Faculty funded full time technician. The microscope will be used to image a range of different specimens carried out by numerous researchers and postgraduate students. Examples of specimens that will be imaged include three dimensional collagenous scaffolds which are used in tissue engineering, cells adhering to and growing in tissue engineering scaffolds, nanoparticles produced by biomaterials used in hip and knee replacements, nanoparticles in body tissues and in environmental samples, virus particles, fibrils of proteins that cause disease such as amyloid and prion proteins and proteins that cause muscles to contract and bacteria to move.

场发射枪扫描电子显微镜（FEG-SEM）是一种能够产生样品表面非常高分辨率图像的显微镜。它在生物和材料科学中有着广泛的应用，研究人员希望在广泛的放大倍数范围内可视化和分析样品的表面。场发射枪扫描电子显微镜可用于在大的表面积上成像，可用于对块状材料以及薄膜或斑点成像，现代显微镜可以对小至1或2纳米的结构成像。传统的FEG-SEM要求样品在高真空下成像，这意味着样品，例如潮湿的生物材料，会产生大量干扰图像的蒸汽。为了通过传统的FEG-SEM可视化生物样本，样本必须被干燥和涂覆，这可能会扭曲结构的图像。SEM的另一种形式，称为环境SEM（ESEM），允许样品在低压气体环境和高湿度下可视化，这意味着生物样品可以直接或在冷冻状态下以其水合状态成像。在此应用中，我们正在寻求取代传统的SEM，这是27岁，仍然需要胶片处理（不获取数字图像），并经常发生故障。我们希望购买一台多功能高分辨率低真空FEG-SEM和一台低温工作站。显微镜是迫切需要大量的当前和未来的项目在生物科学，特别是研究组织工程，生物材料，结构分子和细胞生物学。所要求的显微镜是最通用的高分辨率场发射枪电子显微镜，具有扩展的低真空能力。它的一个主要特点是使用单一工具，而过去需要多个系统。扫描电子显微镜有三种工作模式：高真空、低真空和环境扫描（ESEM）。在不同模式下可实现的分辨率为1纳米。该设备将直接取代生物科学学院现有电子显微镜单元中的旧SEM。不需要翻修。设备的使用将由学院资助的全职技术人员提供支持。该显微镜将被用于成像一系列不同的标本进行了许多研究人员和研究生。将被成像的样本的实例包括用于组织工程的三维胶原支架、粘附并生长在组织工程支架中的细胞、由用于髋关节和膝关节置换的生物材料产生的纳米颗粒、身体组织和环境样品中的纳米颗粒、病毒颗粒、引起疾病的蛋白质纤维，如淀粉样蛋白和朊病毒蛋白，以及引起肌肉收缩和细菌移动的蛋白质。

项目成果

Biological effects of cobalt-chromium nanoparticles and ions on dural fibroblasts and dural epithelial cells

• DOI: 10.1016/j.biomaterials.2013.01.023
• 发表时间: 2013-05-01
• 期刊: BIOMATERIALS
• 影响因子: 14
• 作者: Behl, Bharat;Papageorgiou, Iraklis;Ingham, Eileen
• 通讯作者: Ingham, Eileen

Analysis of wear, wear particles, and reduced inflammatory potential of vitamin E ultrahigh-molecular-weight polyethylene for use in total joint replacement.

• DOI: 10.1002/jbm.b.32904
• 发表时间: 2013-04
• 期刊: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
• 影响因子: 3.4
• 作者: Bladen, C. L.;Teramura, S.;Russell, S. L.;Fujiwara, K.;Fisher, J.;Ingham, E.;Tomita, N.;Tipper, J. L.
• 通讯作者: Tipper, J. L.

Technique for internal channelling of hydroentangled nonwoven scaffolds to enhance cell penetration.

用于内部通道非织造支架的内部通道技术，以增强细胞渗透。

• DOI: 10.1177/0885328212445077
• 发表时间: 2013-08
• 期刊: Journal of biomaterials applications
• 影响因子: 2.9
• 作者: Durham ER;Ingham E;Russell SJ
• 通讯作者: Russell SJ