High-resolution imaging of mammalian cells by confocal microscopy

通过共焦显微镜对哺乳动物细胞进行高分辨率成像

• 批准号: BB/F011431/1
• 负责人: Anne Ridley
• 金额: $ 30.63万
• 依托单位: King's College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2008
• 资助国家: 英国
• 起止时间: 2008 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FF011431%2F1
• 关键词: resolution; imaging; mammalian; cells; confocal

In animals, cells need to move during development and in response to infection or tissue damage. Cell movement is driven by the cytoskeleton, principally by filaments made of actin molecules, and also requires changes in the way cells attach to other cells or to molecules around them. We want to understand more about how proteins inside cells drive or inhibit cell movement, and therefore be able to more effectively use these proteins as targets for drug therapy against several diseases including chronic inflammatory disorders, heart disease, Alzheimer's and some cancers.

在动物中，细胞在发育过程中需要移动，以应对感染或组织损伤。细胞运动是由细胞骨架驱动的，主要是由肌动蛋白分子构成的细丝，同时也需要改变细胞附着在其他细胞或周围分子上的方式。我们希望更多地了解细胞内的蛋白质如何驱动或抑制细胞运动，从而能够更有效地利用这些蛋白质作为药物治疗多种疾病的靶点，包括慢性炎症性疾病，心脏病，阿尔茨海默氏症和一些癌症。

项目成果

Lamellipodin and the Scar/WAVE complex cooperate to promote cell migration in vivo.

• DOI: 10.1083/jcb.201304051
• 发表时间: 2013-11-25
• 期刊: The Journal of cell biology
• 作者: Law AL;Vehlow A;Kotini M;Dodgson L;Soong D;Theveneau E;Bodo C;Taylor E;Navarro C;Perera U;Michael M;Dunn GA;Bennett D;Mayor R;Krause M
• 通讯作者: Krause M

Nance-Horan Syndrome-like 1 protein negatively regulates Scar/WAVE-Arp2/3 activity and inhibits lamellipodia stability and cell migration.

• DOI: 10.1038/s41467-021-25916-6
• 发表时间: 2021-09-28
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Law AL;Jalal S;Pallett T;Mosis F;Guni A;Brayford S;Yolland L;Marcotti S;Levitt JA;Poland SP;Rowe-Sampson M;Jandke A;Köchl R;Pula G;Ameer-Beg SM;Stramer BM;Krause M
• 通讯作者: Krause M

Lamellipodin-RICTOR Signaling Mediates Glioblastoma Cell Invasion and Radiosensitivity Downstream of EGFR.

• DOI: 10.3390/cancers13215337
• 发表时间: 2021-10-24
• 期刊: Cancers
• 影响因子: 5.2
• 作者: Moritz S;Krause M;Schlatter J;Cordes N;Vehlow A
• 通讯作者: Vehlow A

Endophilin, Lamellipodin, and Mena cooperate to regulate F-actin-dependent EGF-receptor endocytosis.

• DOI: 10.1038/emboj.2013.212
• 发表时间: 2013-10-16
• 期刊: EMBO JOURNAL
• 影响因子: 11.4
• 作者: Vehlow, Anne;Soong, Daniel;Vizcay-Barrena, Gema;Bodo, Cristian;Law, Ah-Lai;Perera, Upamali;Krause, Matthias
• 通讯作者: Krause, Matthias

Nance-Horan Syndrome-like 1 protein negatively regulates Scar/WAVE-Arp2/3 activity and inhibits lamellipodia stability and cell migration

Nance-Horan 综合征样 1 蛋白负向调节 Scar/WAVE-Arp2/3 活性并抑制片状伪足稳定性和细胞迁移

• DOI: 10.1101/2020.05.11.083030
• 发表时间: 2020
• 作者: Law A