STUDIES OF YEAST CDC14

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。我们使用我们新开发的分离gfp标记蛋白的方法来研究细胞周期进程所需的磷酸酶Cdc14的细胞周期依赖性相互作用。对两个Cdc14突变体进行了大规模酵母培养，其中一个位于纺锤极，另一个位于细胞质。我们使用涂有我们内部开发的抗gfp抗体的磁珠进行了分离，并鉴定了每种菌株特异性的cdc14相互作用伙伴。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We use our newly developed method for the isolation of GFP-tagged proteins to study the cell-cycle dependent interactions of the phosphatase, Cdc14,required for cell cycle progression. Large scale yeast cultures were grown for two mutants of Cdc14, one localized at the spindle pole and the other with cytoplasmic localization. We have performed isolations using magnetic beads coated with our in-house developed anti-GFP antibodies and identified Cdc14-interacting partners specific for each strain.