STUDIES OF YEAST CDC14

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们使用我们新开发的分离GFP标记蛋白的方法来研究细胞周期所需的磷酸酶CDC14的相互作用。对CDC14的两个突变株进行了大规模酵母培养，一个定位于纺锤体极，另一个定位于细胞质。我们已经使用涂有我们内部开发的抗GFP抗体的磁珠进行了分离，并确定了每个菌株特有的CDC14相互作用伙伴。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We use our newly developed method for the isolation of GFP-tagged proteins to study the cell-cycle dependent interactions of the phosphatase, Cdc14,required for cell cycle progression. Large scale yeast cultures were grown for two mutants of Cdc14, one localized at the spindle pole and the other with cytoplasmic localization. We have performed isolations using magnetic beads coated with our in-house developed anti-GFP antibodies and identified Cdc14-interacting partners specific for each strain.