STUDIES OF YEAST CDC14

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们使用我们新开发的方法分离GFP标记的蛋白质，研究细胞周期进程所需的磷酸酶Cdc 14的细胞周期依赖性相互作用。大规模的酵母培养物生长的Cdc 14的两个突变体，一个定位在纺锤体极和其他细胞质定位。 我们已经使用包被有我们内部开发的抗GFP抗体的磁珠进行了分离，并鉴定了每种菌株特异性的Cdc 14相互作用伴侣。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We use our newly developed method for the isolation of GFP-tagged proteins to study the cell-cycle dependent interactions of the phosphatase, Cdc14,required for cell cycle progression. Large scale yeast cultures were grown for two mutants of Cdc14, one localized at the spindle pole and the other with cytoplasmic localization. We have performed isolations using magnetic beads coated with our in-house developed anti-GFP antibodies and identified Cdc14-interacting partners specific for each strain.