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Characterization of novel Drosophila candidate axon guidance molecules

新型果蝇候选轴突引导分子的表征

基本信息

批准号：
BB/F014287/1
负责人：
Guy Justin Clive Tear
金额：
$ 46.72万
依托单位：
King's College London
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2008
资助国家：
英国
起止时间：
2008 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FF014287%2F1
关键词：
Characterization novel Drosophila candidate axon

项目摘要

We are dependent on our nervous system functioning correctly for us to move, think, learn, speak and control our bodies. To do this all our nerve cells must connect up in the brain and to the parts of the body they control. Most of this 'wiring together' happens during the growth and development of the embryo in pregnancy. To do this each nerve cell must extend a long process, called an axon, over large distances and through complex environments to find and connect to its appropriate partners. Each axon is guided when to turn and which way to grow to reach its partner by sensing specific chemicals or molecular 'cues' in different parts of the body. These signals are detected by 'receptor' proteins at the tip of the growing axon. We want to find the molecules that work as these receptors or cues to guide axons along their pathways. From what we know already many of the same molecules and receptors in mammals are also present in smaller animals like the fruitfly Drosophila where they do the same job but on a simpler scale. However we also know we haven't found all the molecules needed to wire-up the nervous system. To find additional molecules we have searched through the genomes of mouse, human and Drosophila to find molecules that are similar to one another and to those we already know about which are found in the developing nervous system. We plan to use Drosophila as a model system where we can disrupt or remove the function of these new molecules to find out if and how they act to direct nerve growth. We use Drosophila as a model system so we can rapidly test the role of these new molecules and to allow us to reduce the need to sacrifice large numbers of mice in research. Once we have found out how these molecules work in Drosophila we will inform other researchers so that the molecules can be tested in other model systems. We need this information both to learn how the nervous system is made and to find out what molecules might be useful in helping us to repair neural injuries or diseases that lead to paralysis or neural degeneration. Unfortunately mammals cannot repair nerve damage that occurs in the brain, our hope is that by identifying the molecules that were originally used to drive and direct nerve cell growth in the embryo we can re-supply these molecules to help nerve cell regeneration in people.

我们依赖于我们的神经系统正常运作，让我们移动，思考，学习，说话和控制我们的身体。要做到这一点，我们所有的神经细胞都必须连接到大脑和它们控制的身体部位。大多数这种“连接在一起”发生在怀孕期间胚胎的生长和发育过程中。要做到这一点，每个神经细胞必须延伸一个很长的过程，称为轴突，在很长的距离和复杂的环境中找到并连接到合适的伙伴。每个轴突通过感知身体不同部位的特定化学物质或分子“线索”来指导何时转向以及以何种方式生长以到达其伴侣。这些信号被生长轴突顶端的“受体”蛋白检测到。我们希望找到作为这些受体或线索的分子，以引导轴突沿着它们的通路。据我们所知，哺乳动物中的许多相同分子和受体也存在于较小的动物中，如果蝇，它们在那里做同样的工作，但规模更简单。然而，我们也知道我们还没有找到所有的分子需要电线的神经系统。为了找到更多的分子，我们搜索了小鼠、人类和果蝇的基因组，以找到彼此相似的分子，以及我们已经知道的在发育中的神经系统中发现的分子。我们计划使用果蝇作为模型系统，在那里我们可以破坏或去除这些新分子的功能，以发现它们是否以及如何指导神经生长。我们使用果蝇作为模型系统，这样我们就可以快速测试这些新分子的作用，并使我们能够减少在研究中牺牲大量小鼠的需要。一旦我们发现这些分子如何在果蝇中起作用，我们将通知其他研究人员，以便这些分子可以在其他模型系统中进行测试。我们需要这些信息来了解神经系统是如何形成的，并找出哪些分子可能有助于我们修复导致瘫痪或神经退化的神经损伤或疾病。不幸的是，哺乳动物无法修复大脑中发生的神经损伤，我们希望通过识别最初用于驱动和指导胚胎神经细胞生长的分子，我们可以重新供应这些分子，以帮助人类神经细胞再生。

项目成果

期刊论文数量（4）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Analysis of the expression patterns, subcellular localisations and interaction partners of Drosophila proteins using a pigP protein trap library.

DOI：
10.1242/dev.111054
发表时间：
2014-10
期刊：
Development (Cambridge, England)
影响因子：
0
作者：
Lowe N;Rees JS;Roote J;Ryder E;Armean IM;Johnson G;Drummond E;Spriggs H;Drummond J;Magbanua JP;Naylor H;Sanson B;Bastock R;Huelsmann S;Trovisco V;Landgraf M;Knowles-Barley S;Armstrong JD;White-Cooper H;Hansen C;Phillips RG;UK Drosophila Protein Trap Screening Consortium;Lilley KS;Russell S;St Johnston D
通讯作者：
St Johnston D

Mushroom body defect is required in parallel to Netrin for midline axon guidance in Drosophila.

与Netrin平行需要蘑菇体缺陷，以进行果蝇中线轴突指导。