MUTANT MOUSE REGIONAL RESOURCE CENTER

老鼠显然已经成为分析和解释人类基因组计划提供的大量数据的首选模式生物。 诱导突变小鼠的创建已成为常规;然而，这些小鼠的表型表征并没有跟上。 此外，调查人员往往很难将这一重要资源分发给提出要求的其他调查人员。 由于突变小鼠是了解人类疾病和治疗疾病的重要研究工具，Taconic的目标是建立一个区域中心，以帮助这些突变体的表征和分布。 简而言之，我们打算通过使用系统的输入、评估、改进和分配程序来完善资源。 我们将利用现有的屏障和传统的住房，建立和维持通过突变小鼠区域资源中心进口的突变小鼠品系。 这些品系将进行胚胎冷冻保存和再衍生，以提供具有非常高标准健康状况的小鼠。 我们将进行广泛的行为和病理表型和微阵列技术也将作为一种工具进行评估，以遗传特征的突变小鼠的表型。 将评价小鼠的遗传背景，并在必要时进一步确定。 突变小鼠和冷冻胚胎的计算机数据库将是最先进的，我们将使用我们完善的分销网络，根据要求向研究人员提供小鼠。 我们的总体目标是协助突变小鼠的表征和交付给研究界，使这些突变体可能成为更有价值的工具，在阐明哺乳动物体内平衡和疾病的机制。

The mouse has clearly become the model organism of choice for analyzing and interpreting the reams of data provided by the human genome initiative. The creation of induced mutant mice has become routine; however, the phenotypic characterization of these mice has not kept pace. In addition, it is often difficult for investigators to distribute this important resource to other investigators who request them. Since mutant mice are such an important research tool for understanding human disease and for working towards treatments of disease, Taconic's goal is to form a regional center that would aid in the characterization and the distribution of these mutants. In brief, we intend to refine the resource through the use of a systematic importation, evaluation, improvement and distribution procedure. We will use current barrier and traditional housing to establish and maintain mutant lines of mice imported through the Mutant Mouse Regional Resource Center. These lines will be embryo cryopreserved and rederived to provide mice with a very high standard health profile. We will perform extensive behavioral and pathological phenotyping and microarray technology will also be evaluated as a tool to genetically characterize the phenotypes of mutant mice. The genetic background of the mice will be evaluated and further defined as necessary. Computer databases for mutant mice and cryopreserved embryos will be state-of-the-art and we will use our well-established distribution network to deliver mice to investigators upon request. Our overall objective is to assist in the characterization and delivery of mutant mice to the research community so that such mutants may become even more valuable tools in the elucidation of the mechanisms governing mammalian homeostasis and disease.