Exploitation of surface plasmon resonance imaging for two dimensional smart cell screening

将开发一种无运动波长选择性成像表面等离子体共振系统，以可视化表面捕获细胞对刺激的更广泛的新陈代谢驱动和细胞骨架电荷，为优化广泛的生物过程创造一个广泛适用的筛选工具。该系统在设计上是创新的，因为它没有运动部件，在应用上也很新颖，因为它将使细胞表面接触电荷能够同时监测(不同种类的)表面贴壁细胞群体。当表面的特定细胞亚群记录了对预先选择的外部刺激(例如，酶底物)的反应时，该细胞群为进一步优化特定受体或酶的级联提供了预先选择的面板。预计该系统将应用于发现有利于各种生物处理的环境，其稳健性将有助于与用于反馈控制和生物优化的生物过程相结合。

A motion free wavelength selective imaging surface plasmon resonance system will be developed to visualise the broader metabolically driven and cytoskeletal charges of surface captured cells in response to stimuli creating a broadly applicable screening tool for optimisation of a wide range of bioprocesses. The proposed system is innovative in design in that it has no moving parts and novel in application in that it will enable cell surface contact charges to be monitored simultaneously for a (heterogeneous) population of surface adherent cells. When response to a pre-selected external stimulus (e.g. enzyme substrate) is registered by a specific cellsub-group at the surface, then that cell group provides a preselected panel for further optimisation for a particular receptor or enzyme cascade. It is envisaged that the system will have application in the discovery of environments conducive to a diverse range of bioprocessing and the robustness would lend itself to integration with the bioprocesses for feedback control and bio-optimisation.