Exploitation of surface plasmon resonance imaging for two dimensional smart cell screening

利用表面等离子共振成像进行二维智能细胞筛选

• 批准号: DT/E010857/1
• 负责人: Asim Ray
• 金额: $ 26.52万
• 依托单位: Queen Mary University of London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=DT%2FE010857%2F1
• 关键词: Exploitation; surface; plasmon; resonance; imaging

A motion free wavelength selective imaging surface plasmon resonance system will be developed to visualise the broader metabolically driven and cytoskeletal charges of surface captured cells in response to stimuli creating a broadly applicable screening tool for optimisation of a wide range of bioprocesses. The proposed system is innovative in design in that it has no moving parts and novel in application in that it will enable cell surface contact charges to be monitored simultaneously for a (heterogeneous) population of surface adherent cells. When response to a pre-selected external stimulus (e.g. enzyme substrate) is registered by a specific cellsub-group at the surface, then that cell group provides a preselected panel for further optimisation for a particular receptor or enzyme cascade. It is envisaged that the system will have application in the discovery of environments conducive to a diverse range of bioprocessing and the robustness would lend itself to integration with the bioprocesses for feedback control and bio-optimisation.

将开发一种无运动波长选择性成像表面等离子体共振系统，以可视化响应刺激的表面捕获细胞的更广泛的代谢驱动和细胞骨架电荷，从而创建广泛适用的筛选工具，用于优化广泛的生物过程。所提出的系统在设计上是创新的，因为它没有移动部件，并且在应用上是新颖的，因为它将使得能够同时监测表面粘附细胞的（异质）群体的细胞表面接触电荷。当对预先选择的外部刺激（例如酶底物）的响应被表面上的特定细胞亚群记录时，则该细胞群提供预先选择的面板以进一步优化特定受体或酶级联。据设想，该系统将在发现有利于不同范围的生物加工的环境中具有应用，并且鲁棒性将使其自身与用于反馈控制和生物优化的生物过程集成。