VIRAL PROTEASE VP4

病毒蛋白酶 VP4

• 批准号: 7358944
• 负责人: MARK WILLIAM PAETZEL
• 金额: $ 0.86万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7358944
• 关键词: VIRAL; PROTEASE; VP4

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are studying a novel viral protease. This protease is a self-processing type, and it cleaves itself at its N- and C- terminus. It has a unique type of active site, not found in any other group of viral proteases. Our goal is to solve the structure of such viral protease, in order to gain insights into the details of its mechanisms. In addition, these proteases are potential drug targets, considering that the virus infects farmed fish. We have Seleno-Met crystals of the protease, that belong to space group P6122. These crystals diffract to better than 2.5A at the home source. We also have native crystals of P6122, that diffract to 2A at the home source. in addition we have some Seleno-Met triclinic crystals of the same protease. The strategy for solving the structure is a MAD / SAD solution. We hope that one of these approaches will allow us to solve the structure of these viral proteases. We are also including 2 larger native crystals (P6122) for a high resolution data set, if possible.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。我们正在研究一种新的病毒蛋白酶。这种蛋白酶是一种自我加工类型，它在其N-和C-末端切割自己。它有一个独特的活性位点，在任何其他病毒蛋白酶中都没有发现。我们的目标是解决这种病毒蛋白酶的结构，以深入了解其机制的细节。此外，考虑到病毒会感染养殖鱼类，这些蛋白酶是潜在的药物靶点。我们有蛋白酶的硒蛋氨酸晶体，属于空间群P6122。这些晶体在家庭源处的电阻大于2.5A。我们也有P6122的天然晶体，它在家庭来源处可转换为2A。 此外，我们还有一些相同蛋白酶的硒-甲硫氨酸三斜晶体。求解结构的策略是MAD / SAD解。 我们希望这些方法之一将使我们能够解决这些病毒蛋白酶的结构。如果可能的话，我们还包括2个较大的天然晶体（P6122），以获得高分辨率数据集。