X29, OPERATING AT HIGH SPEED

X29，高速运行

• 批准号: 7358962
• 负责人: HOWARD T ROBINSON
• 金额: $ 11.94万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7358962
• 关键词: X29; OPERATING; SPEED

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objectives ¿ X29 has been developed to provide high speed synchrotron access for macromolecular crystallography for both dedicated Protein-Structure Initiative applications, such as its PRT partner, NYSGXRC, and also for the broader General User crystallography community. Results ¿ The "High Speed" aspect of beam line X29 has permeated our operations and development program in 2005. We have systematically improved and optimized instrumentation, software, and the general environment for user operations in our quest for high productivity from our investments at X29. The X29 operations team has worked in conjunction with specialists within the PXRR, the NSLS, the user community and operators of other beam lines around the world to steer this refinement. We have joined the NSLS in addressing improved user access with improvements to the NSLS¿s Proposal, Allocation, Safety, and Scheduling system. An important advance has been implementation of the "Macromolecular Envelope Safety Approval." However, our own "Rapid Access" system continues to provide the proposal and scheduling venue for most user access to PXRR beam lines until PASS is working properly. Our program continues to drive the unification of beam time-access portals within the NSLS. The PXRR is now providing a streamlined request avenue for users of the NSLS bend magnet beam lines to bring their demanding crystals to the PXRR insertion device beam lines (X29 and later X25) for two-to-four-hour beam-time slots. In particular, there is now an ¿ID quick project¿ request within our data-base-grounded rapid-access procedure that can provide response within minutes. The similarity of approach within the software for operations of the four PXRR bend magnet beam lines allows the users to be able to make productive use of these short slots at X29. Automatic systems within X29 manage the beam line x-ray optics and undulator such that the user is not burdened beyond the selection of wavelength. The mini-Kappa design, combined with the precision air bearing omega spindle, provides both simplified operation (both for loading crystals from liquid nitrogen and also for adjustment of crystal axis orientation) and the precise motions needed for the short exposures commonly employed at X29 (about one second exposure with one degree oscillation). The small scale construction of these parts has allowed for full motion of these mechanisms so the user needs not be concerned about equipment bumping. The large size, readout speed, reliability, and signal-to-noise of the ADSC Q315 detector plays a significant role in providing the user with a fast and high quality data collection environment at X29. Although we have focused the operation of X29 on fast acquisition of standard crystallography experiments, we have found that users with more demanding projects are pleased too. For instance, X29 users with large unit cell crystals are enthusiastic about their ability simply to adjust the horizontal focus of the beam, and to re-position crystal axes with the mini-kappa. Users with very small crystals enjoy the simple adjustment of the aperture. And of course, the high flux benefits all of the users in that it greatly speeds their work. Finally, we have found that the users have responded to the expanding of scheduling models for X29, in that they can more easily fulfill their experimental needs with responsive and fluidized scheduling modes combined with simplified operations for this high flux beam line. We have steadily increased the user support we provide for beam line X29 as the throughput of experimenters at the beam line has increased over the past year. The PXRR currently provides four PX-operators, two on day shifts and two on evening. These PX-operators are the first contact person for users with questions and problems. The PXOps can resolve most problems immediately, while others may require that the PX-operator seek help from our broader support environment. The point generally is to increase the effectiveness of the user's beam time by seamlessly attaching the experience and expertise of the PX-operators. Plans ¿ We know that there are improvements that can be made in the functioning of the monochromator. In particular, we know that if we make precise manual adjustments we can gain twice the intensity. However we don¿t know how to do this automatically. We will study the problem and find a solution. Secondly, we know that we can improve the signal-to-background situation, especially for long-wavelength data, and will study this. An obvious solution will be to introduce a He-filled chamber surrounding the tip of the collimator and the beam stop. Finally, we expect gradually to increase the staffing to lubricate further the interaction among all of our beam lines, producing a ¿sea of photons¿ in which the experimenters can swim.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。目标 X29 的开发目的是为大分子晶体学提供高速同步加速器访问，既适用于专门的蛋白质结构倡议应用，例如其 PRT 合作伙伴 NYSGXRC，也适用于更广泛的一般用户晶体学社区。 结果 ¿ 光束线 X29 的“高速”方面已渗透到我们 2005 年的运营和开发计划中。我们系统地改进和优化了仪器、软件和用户操作的一般环境，以寻求从 X29 的投资中获得高生产率。 X29 运营团队与 PXRR、NSLS、用户社区和世界各地其他光束线运营商的专家合作，指导这一改进。我们加入了 NSLS，通过改进 NSLS 的提案、分配、安全和调度系统来解决改善用户访问的问题。一项重要进展是“高分子包膜安全认证”的实施。然而，我们自己的“快速访问”系统继续为大多数用户访问 PXRR 光束线提供建议和调度场所，直到 PASS 正常工作。我们的计划继续推动 NSLS 内波束时间访问门户的统一。 PXRR 现在为 NSLS 弯曲磁体束线的用户提供简化的请求途径，将其要求严格的晶体带到 PXRR 插入设备束线（X29 和后来的 X25），以获得两到四小时的束时隙。特别是，现在我们基于数据库的快速访问程序中有一个“ID 快速项目”请求，可以在几分钟内提供响应。 软件内用于操作四个 PXRR 弯曲磁体束线的方法的相似性使用户能够高效地利用 X29 上的这些短槽。 X29 内的自动系统管理光束线 X 射线光学器件和波荡器，这样用户就不会因为选择波长而感到负担。 mini-Kappa 设计与精密空气轴承 omega 主轴相结合，提供了简化的操作（既用于从液氮加载晶体，也用于调整晶体轴方向）和 X29 常用的短曝光（大约一秒曝光，一度摆动）所需的精确运动。这些部件的小规模结构允许这些机构完全运动，因此用户无需担心设备碰撞。 ADSC Q315 探测器的大尺寸、读出速度、可靠性和信噪比在 X29 为用户提供快速、高质量的数据采集环境方面发挥着重要作用。 尽管我们将X29的操作重点放在了标准晶体学实验的快速采集上，但我们发现具有更高要求项目的用户也很高兴。例如，拥有大型晶胞晶体的 X29 用户对他们能够简单地调整光束的水平焦点以及使用 mini-kappa 重新定位晶轴的能力充满热情。晶体非常小的用户可以享受简单的光圈调整。当然，高通量对所有用户都有好处，因为它大大加快了他们的工作速度。最后，我们发现用户对 X29 调度模型的扩展做出了反应，因为他们可以通过响应和流化的调度模式结合该高通量光束线的简化操作来更轻松地满足他们的实验需求。 随着光束线 X29 上实验人员的吞吐量在过去一年中不断增加，我们稳步增加了为光束线 X29 提供的用户支持。 PXRR 目前提供四名 PX 操作员，其中两名白班，两名夜班。这些 PX 操作员是用户提出疑问和问题的第一联系人。 PXOps 可以立即解决大多数问题，而其他问题可能需要 PX 操作员从我们更广泛的支持环境中寻求帮助。重点通常是通过无缝结合 PX 操作员的经验和专业知识来提高用户波束时间的有效性。 计划 - 我们知道单色仪的功能可以进行改进。特别是，我们知道，如果我们进行精确的手动调整，我们可以获得两倍的强度。然而我们不知道如何自动执行此操作。我们将研究这个问题并找到解决方案。其次，我们知道我们可以改善信号与背景的情况，特别是对于长波长数据，并将对此进行研究。一个显而易见的解决方案是在准直器尖端和光束挡板周围引入一个充满氦气的室。最后，我们期望逐渐增加人员配备，以进一步润滑我们所有光束线之间的相互作用，产生实验者可以在其中游泳的“光子海洋”。