THE RESPONSE REGULATOR-LIKE DOMAIN OF RSSB

RSSB 的类似响应调节器的域

• 批准号: 7369536
• 负责人: TANIA A BAKER
• 金额: $ 0.07万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7369536
• 关键词: RESPONSE; REGULATOR; LIKE; DOMAIN; RSSB

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. RssB is a regulatory protein involved in the stress response in E. coli. It is phosphorylated at Asp57 of its N-terminal domain by the ArcB histidine kinase. In its phosphorylated from RssB binds to sigma-S regulatory subunit of RNA polymerase and targets sigma-S for degradation by ClpXP. In its non- phosphorylated form RssB does not bind sigma-S, allowing sigma-S to accumulate and exert its effect by turning on stationary phase and stress response genes. We are interested in understanding the structural basis for the interaction of RssB with sigma-S, the kinase, and ClpX.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。 RSSB是参与大肠杆菌应力反应的调节蛋白。它通过ARCB组氨酸激酶在其N末端结构域的ASP57处磷酸化。在其从RSSB的磷酸化中，与RNA聚合酶的Sigma-S调节亚基结合，并靶向Sigma-S以clpxp降解。在其非磷酸化形式中，RSSB不结合Sigma-S，从而使Sigma-S通过打开固定相和应力响应基因来积累并发挥其作用。我们有兴趣了解RSSB与Sigma-S，激酶和CLPX相互作用的结构基础。