Assembly of the 30S ribosomal subunit

此申请概述了一个合作研究计划将由首席研究员进行 （威廉姆森）和阿列克谢·卡诺霍夫博士在俄罗斯普希奇诺的细胞生物物理研究所。的 父母补助金是GM-53757，“装配的SOS核糖体亚基”。 父母资助的总体目标是研究SOS核糖体的组装机制， 它由一个大的16 S核糖体RNA和20个小的蛋白质组成。SOS亚基可以是 在体外重建，和父母赠款的主要目标之一是研究动力学的过程。 已开发出同位素脉冲追踪测定法，其允许监测所有20种蛋白质的结合率 同步动力学极其复杂，观察到平行和顺序结合。 有许多中间体形成，动力学是非常复杂的。 FIRCA应用程序的目标是与Karnaukhov博士合作，他是该领域的专家。 非线性动态数据分析他开发了新的分析方法，可以直接 应用于核糖体组装的问题。我们已经开始合作开发新的 基于这一共同兴趣的复杂动力学数据分析工具。本申请旨在 支持这项有趣而重要的工作。这项工作对核糖体特别重要 组装问题，更普遍的意义是，因为我们开发的工具将有广泛的 适用于理解化学，生物化学和细胞代谢中的复杂反应动力学。 这项研究将主要在俄罗斯的俄罗斯科学院进行， 博士James R.威廉姆森作为NIH补助金 * R 01 GM 053757的扩展。

This application outlines a collaborative research plan to be carried out by the Principal Investigator (Williamson) with Dr. Alexey Karnaukhov at the Institute of Cell Biophysics in Pushchino, Russia. The parent grant is GM-53757, "Assembly of the SOSribosomal subunit". The overall goal of the parent grant is to study the mechanism of assembly of the SOS ribosome, which is composed of a large 16S ribosomal RNA, and 20 small proteins. The SOS subunit can be reconstituted in vitro, and one of the major goals of the parent grant is to study the kinetics of the process. An isotope pulse chase assay has been developed that allows monitoring of binding rate of all 20 proteins simultaneously. The kinetics are extremely complex, and both parallel and sequential binding is observed. There are many intermediates formed, and the kinetics are extremely complex. The goal of the FIRCA application is to collaborate with Dr. Karnaukhov, who is an expert at the analysis of nonlinear dynamic data. He has developed new analytical approaches that can be directly applied to the problem of ribosome assembly. We have already initiated a collaboration to develop new tools for the analysis of complex kinetic data based on this common interest. This application seeks to support this interesting and important work. The proposed work is specifically significant for the ribosome assembly problem, and is more generally significant because the tools we develop will have broad applicability in understanding complex reaction dynamics in chemistry, biochemistry, and cell metabolism. The research will be done primarily in Russia at the Russian Academy of Sciences, in collaboration with Dr. James R. Williamson as an extension of NIH grant* R01 GM053757.