Dynamics of the Bacterial Ribosome and Proteome
细菌核糖体和蛋白质组的动力学
基本信息
- 批准号:10589145
- 负责人:
- 金额:$ 71.36万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-04-01 至 2025-03-31
- 项目状态:未结题
- 来源:
- 关键词:Applications GrantsBacteriaBacterial ProteinsBudgetsCell physiologyCellsComplexCryo-electron tomographyEconomicsElectron MicroscopyEscherichia coliFluorescenceFluorescence MicroscopyGoalsGrowthIn VitroIndividualMass Spectrum AnalysisMeasurementModelingMonitorNutrientPathway interactionsProcessProtein BiosynthesisProteinsProteomeRibosomesStructurebiophysical propertiesfitnesslight microscopyprotein degradationresponsesingle molecule
项目摘要
PROJECT SUMMARY
This application describes broadly based biophysical characterization of two important aspects
of bacterial cell physiology. First, the dynamics of the entire E. coli proteome will be quantified
as a function of growth rate and nutrient limitation, including measurement of protein turnover.
The set of bacterial proteins are carefully controlled to optimize the fitness and growth of
bacteria under a wide variety of conditions. The amount of protein devoted to various
biosynthetic pathway varies in a defined way that can be simply modeled as an economic
allocation problem. Second, the process of ribosome assembly will be investigated using
electron microscopy, mass spectrometry, single molecule fluorescence, and light microscopy.
Electron microscopy of intermediates that accumulate under perturbed conditions reveals a
distribution of structures that can be ordered into a putative assembly pathway. Single molecule
fluorescence allows the monitoring of individual steps in the complex assembly process.
Finally, we will extend the in vitro studies toward studying the ultrastructure of the locus of
ribosome assembly in cells, using a combination of light microscopy and cryo-electron
tomography. The overall goal is to develop a complete mechanistic framework for assembly of
the largest cellular machine, that is responsible for all protein synthesis, and that requires
almost a third of the energy budget for a rapidly dividing cell.
项目总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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James R Williamson其他文献
James R Williamson的其他文献
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{{ truncateString('James R Williamson', 18)}}的其他基金
Initiation of HIV Capsid Assembly Monitored by Mass Photometry
通过质谱光度法监测 HIV 衣壳组装的启动
- 批准号:
10649361 - 财政年份:2023
- 资助金额:
$ 71.36万 - 项目类别:
Dynamics of the Bacterial Ribosome and Proteome (Equipment Supplement)
细菌核糖体和蛋白质组的动力学(设备补充)
- 批准号:
10799477 - 财政年份:2020
- 资助金额:
$ 71.36万 - 项目类别:
Dynamics of the Bacterial Ribosome and Proteome
细菌核糖体和蛋白质组的动力学
- 批准号:
10380121 - 财政年份:2020
- 资助金额:
$ 71.36万 - 项目类别:
Health-Related Research Experiences for Undergraduates (H-REU) Program
本科生健康相关研究经历(H-REU)计划
- 批准号:
9461598 - 财政年份:2014
- 资助金额:
$ 71.36万 - 项目类别:
Health-Related Research Experiences for Undergraduates (H-REU) Program
本科生健康相关研究经历(H-REU)计划
- 批准号:
8616581 - 财政年份:2014
- 资助金额:
$ 71.36万 - 项目类别:
Health-Related Research Experiences for Undergraduates (H-REU) Program
本科生健康相关研究经历(H-REU)计划
- 批准号:
9045700 - 财政年份:2014
- 资助金额:
$ 71.36万 - 项目类别:
Ribonucleoprotein Complexes Regulating T-Cell Activation
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8153407 - 财政年份:2010
- 资助金额:
$ 71.36万 - 项目类别:
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