Isotopically Labeled Nucleotides for Biomolecular NMR

描述（由申请人提供）：拟议研究的目标是扩大含有稳定同位素标记的高价值核糖核苷三磷酸的生产和制造。这些产品的用途是使用核磁共振（NMR）光谱对RNA结构进行生物分子研究。这些产品的市场是学术和工业实验室中从事RNA结构测定或核磁共振筛选的结构生物学小组。 用13C、15N和2H进行稳定同位素标记是多维NMR研究应用于大分子结构生物学的要求。用于标记RNA和DNA的现有方法具有三个主要缺点。首先，同位素标记的核苷酸的生产是复杂和耗时的，并且难以在单个实验室中进行。其次，它非常昂贵，因为单个样品的材料可能花费数千美元。第三，现有的方法只允许均匀的同位素标记，而选择性同位素标记具有相当大的优势。 最近，我们已经开发出能够改善或消除这三个缺点的技术。 该技术涉及使用一系列酶促转化从标记的前体有效地单锅合成标记的核糖核苷酸。为了使这些试剂广泛用于结构生物学社区，我们建议优化和扩大这些试剂的生产以用于商业分销。

DESCRIPTION (provided by applicant): The goal of the proposed research is to scale up the production and manufacture of high-value ribonucleoside triphosphates containing stable isotope labels. The use for these products is in biomolecular studies of RNA structure using Nuclear Magnetic Resonance (NMR) spectroscopy. The market for these products is structural biology groups in academic and industrial laboratories engaged in structure determination or NMR screening of RNA. Stable isotope labeling with 13C, 15N, and 2H is a requirement for application of multidimensional NMR studies for structural biology of macromolecules. Existing methods for labeling of RNA and DNA suffer from three main drawbacks. First, the production of isotopically labeled nucleotides is complex and time consuming and difficult to carry out in individual laboratories. Second, it is extremely expensive, since the materials for a single sample can cost many thousands of dollars. Third, the existing methods only permit uniform isotope labeling, while there is considerable advantage to selective isotope labeling. Recently, we have developed technology that will ameliorate or eliminate these three drawbacks. The technology involves the efficient single-pot synthesis of labeled ribonucleotides from labeled precursors using a series of enzymatic transformations. In order to make these reagents widely available to the structural biology community, we propose to optimize and scale up production of these reagents for commercial distribution.