Assembly of the 30S ribosomal subunit

描述（由申请人提供）：本申请概述了将由主要研究者（威廉姆森）与俄罗斯普希奇诺细胞生物物理研究所的Alexey Karnaukhov博士进行的合作研究计划。母基金是GM-53757，“SOS核糖体亚基的组装”。该基金的总体目标是研究SOS核糖体的组装机制，SOS核糖体由一个大的16 S核糖体RNA和20个小蛋白质组成。SOS亚基可以在体外重建，父母资助的主要目标之一是研究该过程的动力学。已经开发了同位素脉冲追踪测定，其允许同时监测所有20种蛋白质的结合率。动力学极其复杂，观察到平行和顺序结合。有许多中间体形成，动力学是非常复杂的。FIRCA应用程序的目标是与Karnaukhov博士合作，他是非线性动态数据分析的专家。他开发了新的分析方法，可以直接应用于核糖体组装的问题。我们已经启动了一项合作，以开发基于这一共同兴趣的复杂动力学数据分析新工具。本应用程序旨在支持这项有趣而重要的工作。所提出的工作是特别重要的核糖体组装问题，更普遍的意义，因为我们开发的工具将有广泛的适用性，在理解复杂的反应动力学化学，生物化学和细胞代谢。这项研究将主要在俄罗斯的俄罗斯科学院进行，与詹姆斯·R·威廉姆森作为NIH补助金 * R 01 GM 053757的扩展。

DESCRIPTION (provided by applicant): This application outlines a collaborative research plan to be carried out by the Principal Investigator (Williamson) with Dr. Alexey Karnaukhov at the Institute of Cell Biophysics in Pushchino, Russia. The parent grant is GM-53757, "Assembly of the SOS ribosomal subunit". The overall goal of the parent grant is to study the mechanism of assembly of the SOS ribosome, which is composed of a large 16S ribosomal RNA, and 20 small proteins. The SOS subunit can be reconstituted in vitro, and one of the major goals of the parent grant is to study the kinetics of the process. An isotope pulse chase assay has been developed that allows monitoring of binding rate of all 20 proteins simultaneously. The kinetics are extremely complex, and both parallel and sequential binding is observed. There are many intermediates formed, and the kinetics are extremely complex. The goal of the FIRCA application is to collaborate with Dr. Karnaukhov, who is an expert at the analysis of nonlinear dynamic data. He has developed new analytical approaches that can be directly applied to the problem of ribosome assembly. We have already initiated a collaboration to develop new tools for the analysis of complex kinetic data based on this common interest. This application seeks to support this interesting and important work. The proposed work is specifically significant for the ribosome assembly problem, and is more generally significant because the tools we develop will have broad applicability in understanding complex reaction dynamics in chemistry, biochemistry, and cell metabolism. The research will be done primarily in Russia at the Russian Academy of Sciences, in collaboration with Dr. James R. Williamson as an extension of NIH grant* R01 GM053757.