FACS

流式细胞仪

• 批准号: 7300785
• 负责人: Ralph Marvin Steinman
• 金额: $ 19.28万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7300785
• 关键词: 5-(6)-carboxyfluorescein diacetate succinimidyl ester; Animals; Antibodies; Antigen Presentation; Antigen-Antibody Complex; Antigens; Arts; CD4 Positive T Lymphocytes; CD44 gene; CD8B1 gene; Calibration; Caring; Cell Count; Cell Culture System; Cell Maturation; Cell Separation; Cell surface; Cells; Clinical Investigator; Core Facility; Daily; Data; Data Analyses; Dendritic Cells; Dextrans; Faculty; Family; Flow Cytometry; Fluorescein-5-isothiocyanate; Genetic Polymorphism; IL2RA gene; In Vitro; Instruction; Interleukin-10; Label; Latex; Maintenance; Methodology; Monitor; Participant; Peptide/MHC Complex; Phagocytosis; Pinocytosis; Population; Postdoctoral Fellow; Primary Cell Cultures; Process; Property; Reagent; Research; SELL gene; Scientist; Sorting - Cell Movement; Specialist; Stimulus; Students; T-Lymphocyte; TNF gene; Testing; Tracer; Training; Transgenic Organisms; Visit; Work; base; chemokine receptor; cytokine; design; dextran; in vivo; instrument; langerin; programs; receptor; research study; response; uptake

The flow cytometry core will perform flow cytometry based cell sorting experiments for the Projects and assist the project leaders in the design and interpretation of experiments. The core will train students, postdoctoral fellows, research assistants, and clinical investigators in the use of analytical instruments and data analysis. In addition, the core will maintain 2 FACSCaliburs, 1 FACSVantage and IFACSAria , including daily calibration and care, and twice a year preventative maintenance.

流式细胞术核心将执行基于流式细胞术的细胞分选实验