FACS calibur 4 color modular analytical flow cytometer

描述(申请人提供)：我们的用户组研究人类对病原体的免疫反应和患者对癌症的主动免疫。我们将人类树突状细胞(DC)的生物学与它们作为抗原提呈细胞的使用相结合，以监测和操纵人类的免疫反应，包括体内的免疫反应。我们目前的FACS分析工具无法满足我们的资金需求，原因有四个。1)现有的大学运营的FACS设施在使用上有了很大的扩展。2)评估人类免疫反应的能力也同样扩大，特别是因为有了量化抗原特异性T细胞和监测细胞因子在抗原刺激下的积聚的方法。3)在疾病状态下，使用DC来监控和操纵人类免疫反应的关键方面是可行的。现在，通过重组病毒载体和死亡的肿瘤细胞摄取和呈递临床相关抗原后，DC可以大量生成并冷冻保存。这些树突状细胞现在可以用来监测免疫反应，使用基于FACS的分析，对慢性病毒感染(爱泼斯坦-巴尔病毒和HIV-1)和肿瘤(EBV相关恶性肿瘤、黑色素瘤、骨髓瘤)的免疫反应。此外，DC还可用于原位操纵人类免疫反应，用成熟的DC主动免疫癌症患者，用未成熟的DC沉默反应性T细胞。4)博士后和博士后水平的培训计划，包括K08和K23奖学金获得者，越来越多地针对人类DC和人类免疫反应的研究。出于这些原因，我们需要一台FACS Calibur仪器，以满足用户组对人类DC和人类免疫反应的多参数分析的需求，特别是在病原体和主动免疫方案的设置方面。

DESCRIPTION (provided by applicant): Our user group studies the human immune response to pathogens and the active immunization of patients against cancer. We interface the biology of human dendritic cells (DCs) with their use as antigen presenting cells to monitor and manipulate the human immune response, including in vivo. Our current FACS analysis instrument is unable to meet our funded needs for 4 reasons. 1) The existing university-run FACS facility has greatly expanded in use. 2) The capacity to assess the human immune response has likewise expanded, particularly because of methods to quantify antigen-specific T cells and to monitor the intracellular accumulation of cytokines in response to antigen-stimulation. 3) It is feasible to use DCs to monitor and manipulate critical aspects of the human immune response in disease states. DCs can now be generated in large numbers and cryopreserved following their uptake and presentation of clinically relevant antigens delivered via recombinant viral vectors and dead tumor cells. These DCs can now be used to monitor the immune response, using FACS-based assays, to chronic viral infections (Epstein Barr Virus and HIV-1) and to tumors (EBV-associated malignancy, melanoma, myeloma). Furthermore DCs can be used to manipulate the human immune response in situ, to actively immunize cancer patients with mature DCs and to silence reactive T cells with immature DCs. 4) Training programs at the pre- and postdoctoral level, including awardees of K08 and K23 grants, are increasingly directed to the study of human DCs and the human immune response. For these reasons we are requesting a FACS Calibur instrument to meet the needs of the user group for multiparameter analyses of human DCs and the human immune response, particularly in the setting of pathogens and active immunization protocols.