FACS calibur 4 color modular analytical flow cytometer

描述（由申请人提供）：我们的用户组研究人体对病原体的免疫反应和患者对癌症的主动免疫。我们将人类树突状细胞（DC）的生物学与其作为抗原呈递细胞的用途相结合，以监测和操纵人类免疫应答，包括体内免疫应答。我们目前的FACS分析仪器无法满足我们的资金需求，原因有四个。1)现有的大学管理的外地资产管制系统设施已大大扩大使用。2)评估人类免疫应答的能力也同样扩大，特别是因为定量抗原特异性T细胞和监测响应于抗原刺激的细胞因子的细胞内积累的方法。3)使用DC来监测和操纵疾病状态下人类免疫应答的关键方面是可行的。现在可以大量产生DC，并在其摄取和呈递通过重组病毒载体和死肿瘤细胞递送的临床相关抗原后冷冻保存。这些DC现在可用于使用基于FACS的测定来监测对慢性病毒感染（Epstein巴尔病毒和HIV-1）和肿瘤（EBV相关恶性肿瘤、黑素瘤、骨髓瘤）的免疫应答。此外，DC可用于原位操纵人免疫应答，用成熟DC主动免疫癌症患者，并用未成熟DC沉默反应性T细胞。4)在博士前和博士后水平的培训计划，包括K 08和K23赠款的获奖者，越来越多地针对人类DC和人类免疫应答的研究。出于这些原因，我们正在申请一种FACS Calibur仪器，以满足用户群体对人DC和人免疫应答的多参数分析的需求，特别是在病原体和主动免疫方案的设置中。

DESCRIPTION (provided by applicant): Our user group studies the human immune response to pathogens and the active immunization of patients against cancer. We interface the biology of human dendritic cells (DCs) with their use as antigen presenting cells to monitor and manipulate the human immune response, including in vivo. Our current FACS analysis instrument is unable to meet our funded needs for 4 reasons. 1) The existing university-run FACS facility has greatly expanded in use. 2) The capacity to assess the human immune response has likewise expanded, particularly because of methods to quantify antigen-specific T cells and to monitor the intracellular accumulation of cytokines in response to antigen-stimulation. 3) It is feasible to use DCs to monitor and manipulate critical aspects of the human immune response in disease states. DCs can now be generated in large numbers and cryopreserved following their uptake and presentation of clinically relevant antigens delivered via recombinant viral vectors and dead tumor cells. These DCs can now be used to monitor the immune response, using FACS-based assays, to chronic viral infections (Epstein Barr Virus and HIV-1) and to tumors (EBV-associated malignancy, melanoma, myeloma). Furthermore DCs can be used to manipulate the human immune response in situ, to actively immunize cancer patients with mature DCs and to silence reactive T cells with immature DCs. 4) Training programs at the pre- and postdoctoral level, including awardees of K08 and K23 grants, are increasingly directed to the study of human DCs and the human immune response. For these reasons we are requesting a FACS Calibur instrument to meet the needs of the user group for multiparameter analyses of human DCs and the human immune response, particularly in the setting of pathogens and active immunization protocols.