DETECTION OF MHC CLASS II PEPTIDES

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 应用两步蛋白质组学方法检测树突状细胞处理的 HIV-gag p24 中低水平的 MHC II 类肽。第一步，获得富集的 MHC II 类肽的 MS1 数据，并对 HIV-gag p24 中所有可能的 MHC II 类肽的同位素分布的质量色谱图进行可视化。在第二步中，将第一步中检测到的肽（即具有正确的质量和同位素分布）作为 MS/MS 分析的目标。该方法可以靶向大量肽 (>1000)。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. A two-step proteomics approach was applied to detect low levels of MHC class II peptides from HIV-gag p24 processed by dendritic cells. In the first step MS1 data was obtained for the enriched MHC class II peptides, and the mass chromatograms for the isotope distribution of all possible MHC class II peptides from HIV-gag p24 were visualized. In the second step, the peptides that were detected in step one (i.e. had correct mass and isotope distribution) were targeted for MS/MS analysis. This method allows targeting a large number of peptides (>1000).