DETECTION OF MHC CLASS II PEPTIDES

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 应用两步蛋白质组学方法检测树突状细胞处理的HIV-gag p24中低水平的MHC II类肽。在第一步中，获得富集的MHC II类肽的MS 1数据，并可视化来自HIV-gag p24的所有可能的MHC II类肽的同位素分布的质谱图。在第二步中，将步骤1中检测到的肽（即具有正确的质量和同位素分布）靶向用于MS/MS分析。该方法允许靶向大量肽（>1000）。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. A two-step proteomics approach was applied to detect low levels of MHC class II peptides from HIV-gag p24 processed by dendritic cells. In the first step MS1 data was obtained for the enriched MHC class II peptides, and the mass chromatograms for the isotope distribution of all possible MHC class II peptides from HIV-gag p24 were visualized. In the second step, the peptides that were detected in step one (i.e. had correct mass and isotope distribution) were targeted for MS/MS analysis. This method allows targeting a large number of peptides (>1000).