DETECTION OF MHC CLASS II PEPTIDES

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 应用两步蛋白质组学方法从经树突状细胞处理的HIV-Gag p24中检测到低水平的MHC-II类多肽。在第一步中，获得了富含MHC II类多肽的MS1数据，并可视化了来自HIV-Gag p24的所有可能的MHC II类多肽的同位素分布的质谱图。在第二步中，对第一步中检测到的多肽(即具有正确的质量和同位素分布)进行MS/MS分析。这种方法允许靶向大量的多肽(&gt；1000)。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. A two-step proteomics approach was applied to detect low levels of MHC class II peptides from HIV-gag p24 processed by dendritic cells. In the first step MS1 data was obtained for the enriched MHC class II peptides, and the mass chromatograms for the isotope distribution of all possible MHC class II peptides from HIV-gag p24 were visualized. In the second step, the peptides that were detected in step one (i.e. had correct mass and isotope distribution) were targeted for MS/MS analysis. This method allows targeting a large number of peptides (>1000).