Endoscopically-guided optical coherence tomography for early cancer screening

内窥镜引导光学相干断层扫描用于早期癌症筛查

• 批准号: 7404337
• 负责人: NICUSOR IFTIMIA
• 金额: $ 14.98万
• 依托单位: PHYSICAL SCIENCES, INC
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-11 至 2009-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7404337
• 关键词: Address; Adenocarcinoma; American Type Culture Collection; Animal Model; Animals; Antibodies; Applications Grants; Area; Aspartic Acid; Barrett Esophagus; Binding; Biological; Biopsy; Bladder; Blood; Bos taurus; Caliber; Cancer cell line; Cancerous; Carbocyanines; Carbon Dioxide; Cattle; Cell Line; Cells; Cervical; Cervix Uteri; Charge; Clinical; Clinical Research; Collaborations; Colon; Colon Adenocarcinoma; Colon Carcinoma; Color; Colorectal Cancer; Computer software; Condition; Contrast Media; Cultured Cells; Data Set; Depth; Detection; Development; Diagnosis; Diagnostic; Dyes; Eagles; Effectiveness; Electrons; Endoscopy; Endothelial Cells; Engineering; Enhancers; Epithelial; Epithelial Cells; Esophageal; Esophagus; Evaluation; Fasting; Feasibility Studies; Fluorescence; Fluorescent Dyes; Funding; Gastrointestinal tract structure; Genus Cola; Glycine; Goals; Gold; Government; Guidelines; HeLa S3; Histology; Human; Image; Imagery; Imaging Device; Imaging Techniques; In Vitro; Incubated; Integrins; Invasive; Isoflurane; Label; Lasers; Lateral; Lesion; Ligands; Light; Lighting; Localized; Location; Lung; Malignant Neoplasms; Malignant neoplasm of cervix uteri; Malignant neoplasm of esophagus; Measurement; Methods; Microspheres; Modality; Modeling; Morphology; Mus; Nanosphere; Nanotechnology; Normal Cell; Normal tissue morphology; Optical Coherence Tomography; Optics; Organ; Pancreas; Penicillins; Peptides; Persons; Pharmaceutical Preparations; Phase; Precancerous Conditions; Probability; Process; Purpose; Pyruvate; Pyruvates; Qualifying; RGD (sequence); Rate; Rectal Administration; Research; Research Personnel; Resolution; Sampling; Scanning; Scheme; Score; Screening for cancer; Screening procedure; Sensitivity and Specificity; Serum; Sodium; Solid Neoplasm; Solutions; Source; Specificity; Speed; Staging; Standards of Weights and Measures; Stream; Streptomycin; Structure; Sulfhydryl Compounds; Surface; System; Techniques; Technology; Testing; Therapeutic heat application; Three-Dimensional Image; Three-Dimensional Imaging; Time; Tissues; Topical application; Toxic effect; Translating; United States National Institutes of Health; Universities; Washington; Work; absorption; antibody conjugate; base; bile duct; cancer cell; cancer diagnosis; cancer imaging; colon cancer cell line; data acquisition; design; fetal; flasks; fluorescence imaging; improved; in vivo; instrument; instrumentation; medical schools; micrography; millimeter; mouse model; nano; nanoparticle; neoplastic; neoplastic cell; new technology; novel strategies; physical science; polycaprolactone; programs; receptor; response; size; subcutaneous; success; targeted delivery; tomography; tool; tumor; uptake

DESCRIPTION (provided by applicant): This proposal addresses a need for an improved technology for early cancer detection and rapid screening. Recent advances in nanotechnology and high resolution imaging have led to the development of new investigative tools capable of probing biological tissues at the cellular level. These tools can be used to localize abnormal neoplastic cells and expedite the screening process. Physical Sciences Inc. (PSI), in collaboration with Northeastern University (NEU), proposes to develop a technology that could be used for screening of early stage epithelial pre-cancerous lesions. Enhanced-contrast high-resolution optical coherence tomography (OCT) imaging based on tumor targeted delivery of microparticles will be used to localize abnormal lesions within the tissue. Gold coated microparticles functionalized with epithelial cell specific targeting ligands will be evaluated in vitro and in vivo for specific binding to epithelial targets for early cancer diagnosis. In Phase I, this technology will be tested on various colon cancer cell lines in cultures and in an orthotopic human colon model in mice upon topical administration. The Phase I results and observations will be used to improve the technology in Phase II. A pilot clinical in vivo study on an animal model will be performed to test the effectiveness of this technology.

描述（由申请人提供）：该提案解决了对早期癌症检测和快速筛查的改进技术的需求。纳米技术和高分辨率成像的最新进展导致了能够在细胞水平上探测生物组织的新研究工具的开发。这些工具可用于定位异常肿瘤细胞并加快筛选过程。物理科学公司(PSI)与东北大学（NEU）合作，提出开发一种可用于筛查早期上皮癌前病变的技术。基于微粒的肿瘤靶向递送的增强对比度高分辨率光学相干断层扫描（OCT）成像将用于定位组织内的异常病变。将在体外和体内评价用上皮细胞特异性靶向配体功能化的金包被的微粒与上皮靶的特异性结合，用于早期癌症诊断。在第一阶段，该技术将在培养物中的各种结肠癌细胞系和局部给药后的小鼠原位人结肠模型中进行测试。第一阶段的结果和观察结果将用于改进第二阶段的技术。将在动物模型上进行初步临床体内研究，以测试该技术的有效性。