Imaging fluorescence from cells in 5-dimensions: x, y, wavelength, lifetime, and anisotropy.

对细胞的 5 维荧光进行成像：x、y、波长、寿命和各向异性。

• 批准号: EP/E013422/1
• 负责人: Quentin Hanley
• 金额: $ 15.07万
• 依托单位: Nottingham Trent University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FE013422%2F1
• 关键词: Imaging; fluorescence; cells; dimensions; wavelength

Fluorescent probes are widely used to study the location and function of biomolecules in cells. Using current fluorescence methods, the behavior of molecules within cells can be studied. Examples include: clustering, rotational motion, and intermolecular distance. These three examples are studied by measuring fluorescence lifetime and anisotropy. Simultaneous monitoring of the behavior of many types of molecules in cells over an extended area is an important unsolved physical problem. This proposal seeks to develop, study, and use a new approach to fluorescence imaging combining spectroscopy, fluorescence lifetime, and anisotropy with a goal of studying the behavior of many types of molecules in cells simultaneously. This will produce images with x and y spatial dimensions and fluorescence emission wavelength, fluorescence lifetime, and fluorescence anisotropy. The research has three parts: construction of new instrumentation, tests on defined mixtures of fluorescent compounds in a solution, and study of an important cell surface receptor. Successful completion of the study will provide biologists with new ways to study clustering, motion, and intermolecular distances of multiple molecular species in the field of a microscope.

荧光探针被广泛用于研究生物分子在细胞中的位置和功能。使用当前的荧光方法，可以研究细胞内分子的行为。例子包括：聚类，旋转运动，和分子间距离。通过测量荧光寿命和各向异性来研究这三个例子。同时监测细胞中多种类型分子在扩展区域内的行为是一个重要的未解决的物理问题。该提案旨在开发，研究和使用一种结合光谱学，荧光寿命和各向异性的荧光成像新方法，其目标是同时研究细胞中多种类型分子的行为。这将产生具有x和y空间维度以及荧光发射波长、荧光寿命和荧光各向异性的图像。本研究分为三个部分：新仪器的建设，荧光化合物在溶液中的定义混合物的测试，和一个重要的细胞表面受体的研究。该研究的成功完成将为生物学家提供新的方法来研究显微镜领域中多个分子物种的聚类，运动和分子间距离。

项目成果

Frequency Domain Imaging of Lifetime and Anisotropy Spectra

寿命和各向异性谱的频域成像

• 发表时间: 2008
• 作者: N/a Zhou

Imaging The Anisotropy Spectrum Of Mixtures Of Anti-human IgG-FITC, R6G, and Fluorescent Beads

抗人 IgG-FITC、R6G 和荧光珠混合物的各向异性光谱成像

• 发表时间: 2008
• 作者: N/a Zhou