Nanoactuation of plasma membrane protein clusters

质膜蛋白簇的纳米驱动

• 批准号: EP/H00694X/1
• 负责人: Quentin Hanley
• 金额: $ 25.84万
• 依托单位: Nottingham Trent University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2009
• 资助国家: 英国
• 起止时间: 2009 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FH00694X%2F1
• 关键词: Nanoactuation; plasma; membrane; protein; clusters

Plasma membrane proteins show complex dynamic ordering, such as clustering, on a nanoscale level, which typically changes after external stimulation. The mechanisms of these processes and the consequences for signal transduction are of great interest as extra-cellular signaling events are translated into cells via the plasma membrane. We aim to actuate plasma membrane proteins in a controlled fashion using rationally designed nano-objects and to visualize this interfacing process on the nanometer scale, yielding new insights into cell signalling via the plasma membrane. Rationally designed nano-objects with controlled interfaces to actuate proteins in membranes specifically address the call requirement for groundbreaking research in nanoscale interfaces. The nano-objects bridge the gap between studies on isolated proteins that cannot account for protein clustering in the native environment and whole cell studies, that do not allow the controlled actuation of the nano-clusters.

质膜蛋白质在纳米级水平上表现出复杂的动态有序性，例如聚类，其通常在外部刺激后发生变化。这些过程的机制和信号转导的结果是非常感兴趣的，因为细胞外信号事件通过质膜被翻译到细胞中。我们的目标是使用合理设计的纳米物体以受控的方式驱动质膜蛋白，并在纳米尺度上可视化这种界面过程，从而对通过质膜的细胞信号转导产生新的见解。合理设计的纳米物体与控制界面，以启动蛋白质在膜专门解决呼叫要求突破性的研究，在纳米界面。纳米物体弥合了对分离蛋白质的研究与全细胞研究之间的差距，分离蛋白质不能解释天然环境中的蛋白质聚集，而全细胞研究不允许纳米簇的受控致动。

项目成果

Fluorescence Anisotropy in a Protein: DNA System Undergoing Inducible Assembly

蛋白质中的荧光各向异性：进行诱导组装的 DNA 系统

• DOI: 10.1016/j.bpj.2012.11.2200
• 发表时间: 2013
• 期刊: Biophysical Journal
• 影响因子: 3.4
• 作者: Gholami Z

Theoretical and lab based studies of fluorescence anisotropy toward the analysis of multiple homo-FRET pairs

针对多个同源 FRET 对分析的荧光各向异性的理论和实验室研究

• 发表时间: 2014
• 作者: Zolmajd Haghighi Z.

PNA-protein conjugates for nano-scale modeling of protein aggregates

用于蛋白质聚集体纳米级建模的 PNA-蛋白质缀合物

• 发表时间: 2013
• 作者: Gholami Z.

Enhancement, Equal Fluorescence Efficiency, and Quenching in the Interpretation of Fluorescence Anisotropy Data

荧光各向异性数据解释中的增强、等荧光效率和猝灭

• DOI: 10.1016/j.bpj.2013.11.3767
• 发表时间: 2014
• 期刊: Biophysical Journal
• 影响因子: 3.4
• 作者: Zolmajd-Haghighi Z

Macromolecular binding and kinetic analysis with optically sectioned planar format assays

• DOI: 10.1039/c2an35134j
• 发表时间: 2012-01-01
• 期刊: ANALYST
• 影响因子: 4.2
• 作者: Ghafari, Homanaz;Parambath, Mithun;Hanley, Quentin S.
• 通讯作者: Hanley, Quentin S.