DEVELOPMENTAL RESOURCE FOR BIOPHYSICAL IMAGING AND OPTO ELECTRONICS

生物物理发展资源的总体使命 图像和光电（DRBIO）包括创建， 新仪器和分析的开发和应用 动态细胞的可视化和测量技术 和分子过程。 我们目前的主要研究领域是 多光子激发激光扫描显微镜的发展 （MPELSM），这是我们小组在1990年首次展示的技术， 荧光相关光谱（FCS）的应用 这项技术也是在25年前发展起来的， 发现了许多新的用途， 电脑 多光子荧光成像的优点 激发包括光漂白显著减少， 焦平面外的光损伤（由于强度平方 聚焦光束的依赖性）、激发的更深穿透 光束进入厚，散射样品，并能够激发紫外染料， 而不需要UV光学器件。 我们是一个NCRR资源和执行 与动物和植物生物学研究人员的合作， 化学，物理和医学在康奈尔大学和其他 国家和国际大学。 我们发明的MPELSM 最近获得了BioRad生命科学公司显微镜的许可 现在，它已经是一种商业产品。 的协助下 康奈尔大学生物医学科学家的并行处理资源 理论中心，合作者和工作人员在DRBIO已经制定了常规 使用IBM Data Explorer软件进行重建的方法， 动画的三维图像从多个图像部分，通过 试样

The overall mission of the Developmental Resource for Biophysical Imaging and Opto-Electronics (DRBIO) consists of the creation, development and application of new instrumentation and analysis technologies for the visualization and measurement of dynamic cellular and molecular processes. Our current primary research areas are the development of Multi-Photon Excitation Laser Scanning Microscopy (MPELSM), a technique first demonstrated by our group in 1990, and applications of Fluorescence Correlation Spectroscopy (FCS), a technique also developed in this group 25 years ago which has recently found many new uses with the advent of inexpensive and powerful computers. The advantages of fluorescence imaging with multiphoton excitation include a substantial reduction of photobleaching and photodamage outside of the focal planes (due to the intensity squared dependence of the focused beam), deeper penetration of the excitation beam into thick, scattering samples, and the ability to excite UV dyes without the need for UV optics. We are an NCRR Resource and carry out collaborations with researchers in animal and plant biology, chemistry, physics and medicine here at Cornell and from other national and international universities. Our invention of the MPELSM has recently been licensed by BioRad Life Science's microscopy division and is now a commercial product. With assistance from Parallel Processing Resource for Biomedical Scientists at the Cornell Theory Center, collaborators and staff at DRBIO have developed routine methods using IBM Data Explorer software for the reconstruction and animation of 3D images from multiple image sections through a specimen.