Human studies/biospecimen collection core

核心A，即人类标本-生物标本收集核心，为 通过从一对肥胖的双胞胎中收集粪便样本， (BMI^35）或一致性瘦（BMI 18.5-25）。它利用了一项正在进行的前瞻性研究， 一个由1,800对女性同性双胞胎组成的中西部队列， 率：双胞胎从青春期中期（中位年龄15岁）开始接受随访，预计年龄为25- 35岁， 我们所建议的五年研究的完成时间。核心的具体目标是：目标1，2：Totrace 并对年龄在25-35岁之间的肥胖双胞胎（BMI 30）及其孪生兄弟进行电话筛查访谈， 然后从合格的雌性同性单合子对（第1年：N=20对）和雌性 同性双卵对（第2年：N=20对）-每个合子10个一致肥胖，10个一致瘦 组，每个细胞中有50%是非洲裔美国人，以及来自这些双胞胎的亲生母亲。 排除标准将包括非阴道分娩，近期使用抗生素，早产的对 或在医院重症监护环境中导致围产期护理的分娩并发症， 医疗条件。目的2-4：在一个月内从MZ配对和母亲中获得重复粪便标本。 1个月随访（第1年）、12个月随访（第2年）、24个月随访（第3年）、36个月随访 (year 4）和48个月随访（第5年），以及额外的新鲜粪便样本用于分离 多形拟杆菌和史氏甲烷短杆菌来自8个MZ对及其母亲（1年 和3）.样本采集方案将基于核心主任在远程采集方面的经验， 来自20，000多对双胞胎和亲属的样本正在进行研究。肥胖是一个主要的， 日益严重的公共卫生问题。重复BMI的双胞胎大队列的可用性 基线评估的评估（通过自我报告和报告人- corwin或家长报告） 从他们身上已经发现了500多对肥胖双胞胎，这提供了一个独特的机会， 研究宿主基因型、微生物和宿主x微生物对肥胖风险影响。

Core A, the Human Studies-Biospecimen Collection Core, provides support for the operations of the Program Project through the collection offecal samples from twin pairs who are either concordant obese (BMI^35) or concordant lean (BMI 18.5-25). It takes advantage of an ongoing prospective study of a midwestern cohort of 1,800 female like-sex twin pairs, that has achieved high participation and retention rates: twins are being followed from mid-adolescence (median age 15)and will be of projected ages 25-35by the time of completion of our proposed five-year study. Specific Aims of the core are: Aims 1,2: Totrace and conduct telephone screening interviews with obese twins (BMI¿30) and their cotwins, aged 25-35, and then collect fecal samples from eligible female like-sex monozygotic pairs (year 1:N=20 pairs) and female like-sex dizygotic pairs (year 2:N=20 pairs) - 10concordant obese, 10concordant lean for eachzygosity group ,with 50%of each cell African-American, as well as from the biological mothers of these twins. Exclusionary criteria will include non-vaginal delivery, recent antibiotic use, premature delivery of the pair or birth complications resulting in perinatal care in a hospital's intensive care environment, and certain medical conditions. Aims 2-4: To obtain repeat fecal specimens from the MZpairs and mothers at one- month follow-up (year 1), 12month follow-up (year 2), 24month follow-up (year 3),36 month follow-up (year 4), and 48 month follow-up (year 5), and as well as an additional fresh fecal sample for isolation of strains of Bacteroides thetaiotamicron and Methanobrevibacter smithii from 8 MZpairs and their mothers (years 1 and 3).The sample collection protocol will be based on the core director's experience in remote collectionof samples from over 20,000 twins and relatives in ongoing research studies. Obesity represents a major and growing public health problem. The availability of a large cohort of twin pairs with repeated BMI assessments (byboth self-report and informant - corwin or parent report)from baseline assessment onwards, from whom over 500obese twins have already been identified, offers a unique opportunity to investigate host genotype, microbial, and host x microbial effects on obesity risk.