Acquisition of LMD6000 Laser Microdissection Unit

描述(申请人提供)：所需仪器为徕卡LMD6000多用户激光捕获显微解剖显微镜系统。我们要求该设备的多用户配置，因为它有能力捕获组织和细胞，用于蛋白质组学、遗传学和活细胞亚克隆；这些功能是我们的用户组所需的。目前校园内可用的其他激光捕获系统不足以满足所述项目的需要，因为它们1)速度太慢，2)不能快速处理大范围的组织，3)需要昂贵的技术支持，而且已经排得很紧，4)不能切割植物、胚胎或脱钙骨等硬组织。徕卡LMD6000与其他激光采集设备的显著不同主要在于样品采集机制以及速度和易用性；对于需要高吞吐量的多用户设备而言，这是一个主要优势。加州大学戴维斯分校的15名研究人员需要这些新设备来推进他们的研究项目。其中许多研究人员已经是合作者；11人是NIEHS环境健康科学中心的成员，5人除了各自的资金来源外，还是NIEHS计划项目的联合调查人员。这些用户代表了校园内广泛的科学兴趣，包括生物科学学院(2)、医学院(4)、兽医学院(6)和农业与环境科学学院(3)。这项研究高度关注基础科学和人类健康，包括空气污染物的吸入毒理学、哮喘的动物模型、肺中的干细胞修复、植物和哺乳动物胚胎以及生精小管中的细胞命运模式、心脏病、生育和生殖、一般毒理学、炎症、乳腺癌转移、二恶英对胰腺和肾脏的影响以及角质形成细胞的功能/毒性。所有的建议都有一个共同点，那就是需要激光解剖来快速分析培养或组织中细胞的复杂组合。通过在核心设施中维护这些设备，在CEHS中心的网站上列出这些设备，并在Van Winkle博士的研究生课程中提到这些设备，将有助于向其他校园用户推广这些设备。加州大学戴维斯分校的整体优势是多方面的。这种设备非常适合加州大学戴维斯分校社区的研究需求，其中包括最近对基因组学的重视。

DESCRIPTION (provided by applicant): The instrument requested is a Leica LMD6000 Multi-user Laser Capture Microdissection Microscope System. We are requesting the multi-user configuration of this equipment because it has the capability to capture tissues and cells for proteomics, genetics, and live cell subcloning; features that are required by our user group. The other laser capture systems currently available on campus are not sufficient for the projects described because they 1) are too slow, 2) cannot do large areas of tissue quickly, 3) require expensive technical support and are highly scheduled already, 4) cannot cut hard tissues such as plants, embryos or decalcified bone. The Leica LMD6000 differs significantly from other laser capture units primarily in the mechanism of sample capture as well as speed and ease of use; a major advantage for a multi-user piece of equipment requiring high throughput. The new equipment is needed to advance the research programs of 15 investigators on the UC Davis campus. Many of these investigators are already collaborators; 11 are members of an NIEHS Center for Environmental Health Sciences and 5 are co-investigators on an NIEHS Program project, in addition to their individual funding sources. The users represent a broad range of scientific interest on campus in College of Biosciences (2), School of Medicine (4), School of Veterinary Medicine (6), and College of Agriculture and Environmental Sciences (3). The research is highly focused on both basic sciences and human health and includes studies of inhalation toxicology of air pollutants, animal models of asthma, stem cell repair in the lung, cell fate patterning in plant and mammalian embryos and in seminiferous tubules, heart disease, fertility and reproduction, general toxicology, inflammation, breast cancer metastases, dioxin effects on pancreas and kidney and keratinocyte function/toxicity. All the proposals have one common element, the need for laser dissection to quickly analyze complex composites of cells either in culture or in tissues. Outreach to other campus users will be facilitated by maintaining the equipment in a core facility, listing on the CEHS Center's website, and by mentioning the equipment in Dr. Van Winkle's graduate course. The overall benefits to the UC Davis campus are many. This equipment fits well with the research needs of the UC Davis community, which has included a recent emphasis on genomics.