Acquisition of a Cytoviva hyperspectral microscopy system

摘要 暗视野成像能够检测到组织中的未标记物质。我们所处的体制 Requesting是一种Cytoviva高光谱显微镜系统，结合了专有的暗场 具有高光谱探测功能的显微镜光学系统，可用于成像、测绘和表征 纳米材料(粒子、病毒、细菌)在组织和细胞中的分布。暗场成像 与高光谱成像相结合，允许通过特征来验证对象 光谱特征。专有的聚光器提高了标准暗场的分辨率。 该系统的高光谱方面也可以用来理解组织学染色联合 本地化。在过去的10年里，加州大学戴维斯分校的细胞和分子成像核心(CAMI) 在显微镜和荧光方面的卓越表现与完整的样品制备相结合 能够为超过90个加州大学戴维斯分校用户实验室提供服务。这是一个已建立的共享设施 由使用费(重新收费)支持的资源。细胞活体的能力 这一申请的联合调查人员需要使用该系统。这些主要用户和次要用户 将使用这种仪器来研究纳米材料如何与组织和细胞相互作用，以监测 用于研究炎症细胞生物学的纳米治疗药物的生物利用度和组成 并了解毒理学病理学和病原体反应的细微变化。这 仪器不会复制我们校园中现有的仪器。具备以下附加功能 将随此仪器一起提供包括荧光，以允许荧光共定位 高光谱图像信号是我们的用户所需要的。稳健的图像分析 该系统附带的软件允许对范围广泛的纳米级物体进行光谱识别 基于波长、散射强度和散射空间属性的环境范围。 最重要的是，图像分析软件能够识别 基于峰值波长的光谱图像允许使用标准的组织学方法。 这提供了一种重要的中间分析能力，可替代EM或拉曼 用于样品分析的光谱分析，以较低的成本为用户提供显著的好处 和更快的吞吐量。CAMI工作人员将提供有关成像项目的培训和咨询 使用Cytoviva系统并将该仪器放置在我们的核心设施中是合乎逻辑的 补充我们目前的能力，包括在样品制备方面的卓越表现。这 设备将增加加州大学戴维斯分校NIH资助的研究的影响。

ABSTRACT Darkfield imaging enables detection of unlabeled materials in tissues. The system we are requesting, a CytoViva Hyperspectral Microscopy system, combines proprietary darkfield microscope optics with hyperspectral detection to allow imaging, mapping and characterization of nanoscale materials (particles, viruses, bacteria) in tissues and cells. Darkfield imaging that is coupled with hyperspectral imaging, allows verification of the object through a characteristic spectral profile. The proprietary condenser increases the resolution over standard darkfield. The hyperspectral aspect of this system can also be used to understand histologic staining co- localization. Over the past 10 years, the UC Davis Cellular and Molecular Imaging Core (CAMI) has combined excellence in microscopy and fluorescence with complete sample preparation capabilities to serve over 90 UC Davis user laboratories. This is an established, shared facility resource that is supported with a fee for use (re-charge). The capabilities of the CytoViva system are needed by the co-investigators on this application. These major and minor users will use this instrument to study how nanomaterials interact with tissues and cells, to monitor bioavailability and composition of nanotherapeutics, to study the biology of inflammatory cells and to understand subtle shifts in toxicologic pathology and pathogen responses. This instrument does not duplicate existing instrumentation on our campus. Additional capability that will be supplied with this instrument includes fluorescence to allow colocalization of fluorescent signals with the hyperspectral image as is required by our users. The robust image analysis software included with this system allows spectral identification of nanoscale objects in a wide range of environments based on wavelength, scatter intensity, and scatter spatial properties. Most importantly, the image analysis software enables identification of nanoscale objects in the spectral image based on peak wavelength allowing standard histologic approaches to be used. This provides an important intermediate analysis capability as an alternative to EM or Raman spectroscopy for sample analysis and offers significant benefits to users in terms of lower cost and faster throughput. CAMI staff will provide training and consulting on imaging projects involving the CytoViva system and placement of this instrument in our core facility is a logical complement to our current capabilities which include excellence in sample preparation. This equipment will increase the impact of NIH funded research at UC Davis.