Acquisition of a Cytoviva hyperspectral microscopy system

摘要 暗场成像能够检测组织中的未标记物质。我们的体制 CytoViva高光谱显微镜系统结合了专有的暗场 具有高光谱检测功能的显微镜光学器件，可进行成像、绘图和表征 组织和细胞中的纳米材料（颗粒，病毒，细菌）。暗场成像， 与高光谱成像相结合，可以通过特征验证物体 光谱轮廓专有的聚光镜提高了标准暗场的分辨率。 该系统的高光谱方面也可用于了解组织学染色的共同作用。 本地化在过去的10年里，加州大学戴维斯分校细胞和分子成像核心（CAMI） 结合了显微镜和荧光的卓越性能以及完整的样品制备 为90多个UC Davis用户实验室提供服务。这是一个既定的共享设施 通过使用费用（再收费）支持的资源。CytoViva的功能 共同研究者在这一申请中需要系统。这些主要和次要用户 将使用这种仪器来研究纳米材料如何与组织和细胞相互作用， 纳米治疗剂的生物利用度和组成，以研究炎症细胞的生物学 并了解毒理病理学和病原体反应的微妙变化。这 仪器不重复现有的仪器在我们的校园。附加功能， 将与该仪器一起提供，包括荧光，以允许荧光共定位 高光谱图像的信号，我们的用户所要求的。鲁棒图像分析 该系统包含的软件允许在宽范围内对纳米级物体进行光谱识别。 基于波长、散射强度和散射空间特性的环境范围。 最重要的是，图像分析软件能够识别纳米级物体， 基于峰值波长光谱成像允许使用标准组织学方法。 这提供了重要的中间分析能力，作为EM或拉曼的替代 用于样品分析，并在降低成本方面为用户提供了显着的好处 和更快的吞吐量。CAMI工作人员将提供成像项目的培训和咨询 涉及CytoViva系统并将该仪器放置在我们的核心设施中是合乎逻辑的 补充我们现有的能力，包括卓越的样品制备。这 设备将增加NIH资助的研究在加州大学戴维斯分校的影响。

ABSTRACT Darkfield imaging enables detection of unlabeled materials in tissues. The system we are requesting, a CytoViva Hyperspectral Microscopy system, combines proprietary darkfield microscope optics with hyperspectral detection to allow imaging, mapping and characterization of nanoscale materials (particles, viruses, bacteria) in tissues and cells. Darkfield imaging that is coupled with hyperspectral imaging, allows verification of the object through a characteristic spectral profile. The proprietary condenser increases the resolution over standard darkfield. The hyperspectral aspect of this system can also be used to understand histologic staining co- localization. Over the past 10 years, the UC Davis Cellular and Molecular Imaging Core (CAMI) has combined excellence in microscopy and fluorescence with complete sample preparation capabilities to serve over 90 UC Davis user laboratories. This is an established, shared facility resource that is supported with a fee for use (re-charge). The capabilities of the CytoViva system are needed by the co-investigators on this application. These major and minor users will use this instrument to study how nanomaterials interact with tissues and cells, to monitor bioavailability and composition of nanotherapeutics, to study the biology of inflammatory cells and to understand subtle shifts in toxicologic pathology and pathogen responses. This instrument does not duplicate existing instrumentation on our campus. Additional capability that will be supplied with this instrument includes fluorescence to allow colocalization of fluorescent signals with the hyperspectral image as is required by our users. The robust image analysis software included with this system allows spectral identification of nanoscale objects in a wide range of environments based on wavelength, scatter intensity, and scatter spatial properties. Most importantly, the image analysis software enables identification of nanoscale objects in the spectral image based on peak wavelength allowing standard histologic approaches to be used. This provides an important intermediate analysis capability as an alternative to EM or Raman spectroscopy for sample analysis and offers significant benefits to users in terms of lower cost and faster throughput. CAMI staff will provide training and consulting on imaging projects involving the CytoViva system and placement of this instrument in our core facility is a logical complement to our current capabilities which include excellence in sample preparation. This equipment will increase the impact of NIH funded research at UC Davis.