Acquisition of a Leica TCS LSI Zoom Confocal Microscope

购买 Leica TCS LSI 变焦共焦显微镜

• 批准号: 7791241
• 负责人: Laura S Van Winkle
• 金额: $ 28.65万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7791241
• 关键词: Agriculture; Air Pollutants; Animal Model; Animals; Asthma; Autistic Disorder; Basic Science; Brain; Cardiovascular system; Cells; Childhood; Communities; Complex; Computer software; Core Facility; Disease; Ecology; Educational process of instructing; Elements; Engineering; Equipment; Fishes; Functional disorder; Health; Human; Image; Inhalation Toxicology; Investments; Life; Medicine; Microscope; Neurons; Postdoctoral Fellow; Research; Research Personnel; Resolution; Schedule; Schools; Specimen; Stem cells; System; Time; Tissues; Vascular Diseases; Veterinary Medicine; Work; college; flexibility; graduate student; instrument; interest; outreach; programs

DESCRIPTION (provided by applicant): The instrument requested is a Leica TCS LSI zoom confocal microscope system. We are requesting the current configuration because it has the greatest flexibility in capability and can capture images from live tissues and cells as well as image very large fields of view. The other confocal systems currently available on campus are not sufficient for the projects described because they 1) are too slow, 2) cannot image large fields of view without stitching, 3) are highly scheduled already, 4) are not the correct configuration for our user needs being primarily inverted micrscopes with small working distances and 5) do not have an environmental chamber for imaging large live specimens. There is not currently available on this campus any truly comparable confocal microscope in terms of capability. Hence this microscope will enable studies that were not previously possible or were only achievable in a substandard way with a tremendous investment of both money and time. The Leica TCS LSI differs significantly from other confocals primarily in the ability to image large fields of view and to zoom from low to high magnification. The software is easy to use and the image capture is high resolution and very fast; a major advantage for a multi-user piece of equipment requiring high throughput. The new equipment is needed to advance the research programs of 14 investigators on the UC Davis campus. The users represent a broad range of scientific interest and span four schools/colleges on campus (Medicine, Veterinary Medicine, Agriculture and Environmental Sciences and Engineering). The research is highly focused on both basic sciences and human health and includes studies of inhalation toxicology of air pollutants, animal models of asthma, studies of the circulatory system including heart/vascular disease, brain and nervous system dysfunction, stem cell repopulation of airways and whole animal studies of medaka fish. All the proposals have one common element, the need for a microscope with a large field of view and high resolution to analyze complex composites of cells either in culture or in tissues, quickly. Placement of this equipment in core facilities at UC Davis will also facilitate the projects of graduate students, postdoctoral fellows, residents and professional school (DVM or MD) researchers. Outreach to other campus users will be facilitated by maintaining the equipment in a core facility, listing the equipment on the core and center websites and by inclusion in Dr. Van Winkle's teaching in campus courses on imaging. The overall benefits to the UC Davis campus are many. This equipment fits well with the research needs of the UC Davis community, which has included a recent emphasis on the childhood health disorders autism and asthma that require large field imaging capability for neurons and airways respectively.

描述（由申请人提供）：要求的仪器是Leica TCS LSI变焦共聚焦显微镜系统。我们要求当前的配置，因为它具有最大的灵活性，可以从活组织和细胞中捕获图像，并对非常大的视场进行成像。目前在校园里可用的其他共焦系统不足以用于所描述的项目，因为它们1）太慢，2）在没有拼接的情况下无法成像大视场，3）已经高度调度，4）对于我们的用户需求来说不是正确的配置，主要是工作距离小的倒置显微镜，5）没有用于成像大型活体标本的环境室。目前在这个校园里还没有任何真正可比的共聚焦显微镜的能力。因此，这种显微镜将使以前不可能的研究，或只有在一个不合格的方式与金钱和时间的巨大投资实现。Leica TCS LSI与其他共焦镜的显著不同之处主要在于能够对大视场进行成像并从低放大倍率到高放大倍率进行缩放。该软件易于使用，图像捕获分辨率高且速度非常快;这是需要高吞吐量的多用户设备的主要优势。需要新设备来推进加州大学戴维斯分校校园内14名研究人员的研究计划。用户代表了广泛的科学兴趣，并跨越校园内的四所学校/学院（医学，兽医学，农业和环境科学与工程）。该研究高度关注基础科学和人类健康，包括空气污染物的吸入毒理学研究、哮喘动物模型、包括心脏/血管疾病在内的循环系统研究、脑和神经系统功能障碍、气道干细胞再生和青鳉鱼的整体动物研究。所有这些建议都有一个共同的要素，即需要一种具有大视场和高分辨率的显微镜，以快速分析培养物或组织中复杂的细胞复合物。在加州大学戴维斯分校的核心设施中放置这些设备也将促进研究生，博士后研究员，居民和专业学校（DVM或MD）研究人员的项目。通过维护核心设施中的设备、在核心和中心网站上列出设备以及将其纳入Van Winkle博士的校园成像课程教学中，将有助于与其他校园用户的联系。货车Winkle博士的校园成像课程。加州大学戴维斯分校的整体优势是很多的。该设备非常适合加州大学戴维斯分校社区的研究需求，其中包括最近对儿童健康障碍自闭症和哮喘的重视，这些疾病分别需要神经元和气道的大视场成像能力。