In vivo microcomputed tomography

体内微型计算机断层扫描

• 批准号: 7389369
• 负责人: Kurt David Hankenson
• 金额: $ 34.13万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7389369
• 关键词: Age; Aging; Anesthesia procedures; Animal Model; Animal Welfare; Animals; Cartilage; Complement; Data; Dental; Disease; Engineering; Evaluation; Faculty; Fatty acid glycerol esters; Fluorescence; Grant; Human; Image; Imagery; Imaging technology; Institution; Life; Longitudinal Studies; Malignant Neoplasms; Medical; Metabolic Diseases; Modality; Mus; Muscle; Musculoskeletal; Nuclear; Numbers; Pediatric Hospitals; Pennsylvania; Philadelphia; Rattus; Resolution; Rodent; Scanning; Schools; Services; Site; Skeleton; Structure; System; Tissues; Ultrasonography; Universities; Variant; Veterinary Schools; Week; base; day; digital; in vivo; instrument; member; tomography; tumor

DESCRIPTION (provided by applicant): The primary objective of this application is to purchase an in vivo microcomputed tomography (in vivo microCT) instrument. This instrument permits the three-dimensional visualization of tissue structure at high-resolution (16 micron) in living rodents. Furthermore, the digital data permits highly quantitative determination of a variety of structural parameters. Although used most frequently for analysis of the bony skeleton, microCT can also be used to visualize a variety of other normal and pathological tissues, including cartilage, muscle, fat, vasculature, and tumor. The microCT instrument will be located in the University of Pennsylvania Small Animal Imaging Facility (SAIF) which has an integrated system for longitudinal evaluation of mice and rats using a wide-variety of imaging modalities. High-resolution microCT will represent a significant improvement on the available imaging services and will complement the light-based, fluorescence, ultrasound, and nuclear imaging technologies that already exist in the SAIF. An entire mouse, or part of a rat can be scanned under anesthesia and then the animal recovered, and the same site scanned days, weeks, or months later. Indeed this eliminates the confounding inter-animal variation in longitudinal studies, and importantly, changes with age or treatment in a single animal can be studied. Scanning live animals, repeatedly, in a longitudinal manner is more scientifically robust, and reduces the number of animals needed for any given study. This saves grant dollars, as well the reduction in the number of animals used in a study is good practice for animal welfare. The instrument will be administered by a committee of faculty members drawn from the Engineering, Dental, Medical, and Veterinary Schools at Penn. Similarly, the user group is drawn from these four Schools and also includes users from two other Philadelphia-based institutions, Children's Hospital of Philadelphia (CHOP) and Temple University. High resolution imaging of live rodents is crucial for being able to properly follow the course of experimental disease and aging in a wide-variety of animal models. Information gained from studying rodent musculoskeletal, cancer, and metabolic diseases will have direct translational application to better understanding similar disease states in humans.

描述（由申请人提供）：本申请的主要目的是购买体内微型计算机断层扫描（in vivo microCT）仪器。该仪器可以以高分辨率（16 微米）对活体啮齿动物的组织结构进行三维可视化。此外，数字数据允许高度定量地确定各种结构参数。虽然 microCT 最常用于分析骨骨骼，但它也可用于可视化各种其他正常和病理组织，包括软骨、肌肉、脂肪、脉管系统和肿瘤。 microCT 仪器将位于宾夕法尼亚大学小动物成像设施 (SAIF)，该设施拥有一个集成系统，可使用多种成像方式对小鼠和大鼠进行纵向评估。高分辨率 microCT 将是对现有成像服务的重大改进，并将补充 SAIF 现有的光成像、荧光、超声和核成像技术。可以在麻醉下对整个小鼠或大鼠的一部分进行扫描，然后动物苏醒，并在几天、几周或几个月后扫描同一部位。事实上，这消除了纵向研究中令人困惑的动物间差异，而且重要的是，可以研究单个动物随年龄或治疗的变化。以纵向方式重复扫描活体动物在科学上更加可靠，并且减少了任何特定研究所需的动物数量。这节省了拨款，并且减少研究中使用的动物数量是动物福利的良好做法。该工具将由来自宾夕法尼亚大学工程学院、牙科学院、医学院和兽医学院的教职人员组成的委员会管理。同样，用户群体也来自这四所学校，还包括来自另外两个费城机构费城儿童医院 (CHOP) 和天普大学的用户。 活啮齿动物的高分辨率成像对于能够正确跟踪各种动物模型中的实验疾病和衰老过程至关重要。从研究啮齿动物肌肉骨骼、癌症和代谢疾病中获得的信息将具有直接的转化应用，以更好地了解人类的类似疾病状态。