In vivo microcomputed tomography

体内微型计算机断层扫描

• 批准号: 7389369
• 负责人: Kurt David Hankenson
• 金额: $ 34.13万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7389369
• 关键词: Age; Aging; Anesthesia procedures; Animal Model; Animal Welfare; Animals; Cartilage; Complement; Data; Dental; Disease; Engineering; Evaluation; Faculty; Fatty acid glycerol esters; Fluorescence; Grant; Human; Image; Imagery; Imaging technology; Institution; Life; Longitudinal Studies; Malignant Neoplasms; Medical; Metabolic Diseases; Modality; Mus; Muscle; Musculoskeletal; Nuclear; Numbers; Pediatric Hospitals; Pennsylvania; Philadelphia; Rattus; Resolution; Rodent; Scanning; Schools; Services; Site; Skeleton; Structure; System; Tissues; Ultrasonography; Universities; Variant; Veterinary Schools; Week; base; day; digital; in vivo; instrument; member; tomography; tumor

DESCRIPTION (provided by applicant): The primary objective of this application is to purchase an in vivo microcomputed tomography (in vivo microCT) instrument. This instrument permits the three-dimensional visualization of tissue structure at high-resolution (16 micron) in living rodents. Furthermore, the digital data permits highly quantitative determination of a variety of structural parameters. Although used most frequently for analysis of the bony skeleton, microCT can also be used to visualize a variety of other normal and pathological tissues, including cartilage, muscle, fat, vasculature, and tumor. The microCT instrument will be located in the University of Pennsylvania Small Animal Imaging Facility (SAIF) which has an integrated system for longitudinal evaluation of mice and rats using a wide-variety of imaging modalities. High-resolution microCT will represent a significant improvement on the available imaging services and will complement the light-based, fluorescence, ultrasound, and nuclear imaging technologies that already exist in the SAIF. An entire mouse, or part of a rat can be scanned under anesthesia and then the animal recovered, and the same site scanned days, weeks, or months later. Indeed this eliminates the confounding inter-animal variation in longitudinal studies, and importantly, changes with age or treatment in a single animal can be studied. Scanning live animals, repeatedly, in a longitudinal manner is more scientifically robust, and reduces the number of animals needed for any given study. This saves grant dollars, as well the reduction in the number of animals used in a study is good practice for animal welfare. The instrument will be administered by a committee of faculty members drawn from the Engineering, Dental, Medical, and Veterinary Schools at Penn. Similarly, the user group is drawn from these four Schools and also includes users from two other Philadelphia-based institutions, Children's Hospital of Philadelphia (CHOP) and Temple University. High resolution imaging of live rodents is crucial for being able to properly follow the course of experimental disease and aging in a wide-variety of animal models. Information gained from studying rodent musculoskeletal, cancer, and metabolic diseases will have direct translational application to better understanding similar disease states in humans.

描述（由申请人提供）：本申请的主要目的是购买体内微型层析成像（体内Microct）仪器。该仪器允许在活啮齿动物中高分辨率（16微米）以组织结构的三维可视化。此外，数字数据允许高度定量确定各种结构参数。尽管最常用于分析骨骨架，但Microct也可用于可视化其他正常和病理组织，包括软骨，肌肉，脂肪，脉管系统和肿瘤。 Microct仪器将位于宾夕法尼亚大学小动物成像设施（SAIF）中，该设施具有一个集成的系统，用于使用广泛的成像方式对小鼠和大鼠进行纵向评估。高分辨率Microct将代表可用成像服务的重大改进，并将补充SAIF中已经存在的基于光的，荧光，超声和核成像技术。可以在麻醉下扫描整个老鼠或一部分大鼠，然后恢复动物，并在同一部位扫描几天，几周或几个月后。确实，这消除了纵向研究中的混杂间变异，重要的是，可以研究单个动物的年龄或治疗变化。反复以纵向方式扫描活动物在科学上更强大，并减少了任何给定研究所需的动物数量。这节省了赠款，以及研究中使用的动物数量减少是动物福利的好实践。该仪器将由宾夕法尼亚州工程，牙科，医学和兽医学校提取的教职员工委员会管理。同样，用户组来自这四所学校，还包括来自费城其他两个机构，费城儿童医院（CHOP）和坦普尔大学的用户。 活啮齿动物的高分辨率成像对于能够在广泛的动物模型中正确遵循实验性疾病和衰老的过程至关重要。从研究啮齿动物肌肉骨骼，癌症和代谢疾病中获得的信息将具有直接的转化应用，以更好地理解人类类似的疾病状态。