Translational Immunology Core's FACSAria Cell Sorter

转化免疫学核心的 FACSAria 细胞分选仪

• 批准号: 7390038
• 负责人: FREDERIC Ira PREFFER
• 金额: $ 50万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7390038
• 关键词: Autoimmune Diseases; Autoimmune Process; Back; Basic Science; Bone Marrow Transplantation; Cells; Clinical; Communicable Diseases; Communities; Cytometry; Fees; Financial Support; Flow Cytometry; Funding; General Hospitals; Grant; Immune system; Immunologic Deficiency Syndromes; Immunology; Inflammatory Bowel Diseases; Kidney; Laboratories; Lasers; Malignant Neoplasms; Massachusetts; Medicine; Organ; Pathology; Research; Research Personnel; Resources; Solid; Technology; Today; Translating; United States National Institutes of Health; Work; abstracting; base; experience; instrument; instrumentation; leukemia/lymphoma; multidisciplinary; research facility; tumor immunology

Project Summary/Abstract: Funds are requested to purchase a 4-laser FACSAria from Becton-Dickinson, to be placed in the PI's new Flow Cytometry Core in the Translational Immunology Center [TIC], opening in 2007. The TIC is a new thematic research facility underway at Massachusetts General Hospital's [MGH] Charlestown Navy Yard Research Facility. The TIC will translate advances in basic science to clinical practice in solid organ and bone marrow transplantation, inflammatory bowel disease, tumor immunology and infectious, immunodeficiency and autoimmune diseases. If funded, the requested instrument will serve as the cytometric centerpiece of the TIC's research resource. The PI has an established track record in utilizing polychromatic cytometry for research and clinical work and documents that the requested instrument will provide an essential resource to the needs of the TIC. This instrument will initially serve a multidisciplinary group of 15 primary investigators from 5 departments, representing 67 NIH funded grants; it will further benefit the entire research community located in the Charlestown Research Facility, who presently lack appropriate cytometric instrumentation for their NIH funded work. The PI has served the MGH research community since 1985, and his laboratory has been the only one easily available to all investigators at MGH. This laboratory has a highly skilled operator, who along with the PI, collectively represent well over 40 years of well documented experience in flow cytometry. The laboratory has administered and sustained itself on a combination of user fees, grants and departmental backing when necessary. The laboratory has the documented financial support of both the Department of Pathology and MGH administration. Relevance: This application petitions the NIH for funds to purchase high-technology instrumentation essential to study and understand how cells of the immune system can be used to treat malignancies such as leukemia and lymphoma. Advances in medicine based on similar past work today permit curative kidney and bone marrow transplantations as well as treatments for certain autoimmune and infectious disease.

项目摘要/摘要：需要资金从Becton-Dickinson公司购买一台4激光器FACSAria，放置在翻译免疫中心[TIC]的PI新的流式细胞术核心中，该中心将于2007年开放。TIC是马萨诸塞州总医院[MGH]查尔斯敦海军造船厂研究设施正在进行的一个新的主题研究设施。TIC将把基础科学的进展转化为固体器官和骨髓移植、炎症性肠病、肿瘤免疫学以及传染病、免疫缺陷和自身免疫性疾病的临床实践。如果获得资助，所要求的仪器将成为旅游业议会研究资源的细胞学中心。PI在利用多色细胞术进行研究和临床工作方面有既定的记录，并证明所要求的仪器将为TIC的需要提供必要的资源。该仪器最初将服务于一个由来自5个部门的15名主要研究人员组成的多学科小组，代表着NIH资助的67笔赠款；它将进一步惠及位于查尔斯敦研究设施的整个研究社区，他们目前缺乏适当的细胞学仪器来开展NIH资助的工作。自1985年以来，PI一直服务于MGH研究界，他的实验室一直是MGH所有研究人员都可以轻松获得的唯一实验室。该实验室拥有一名技术精湛的操作员，他们与PI一起，共同代表了40多年来在流式细胞仪方面的良好记录经验。实验室的管理和维持依靠的是使用费、补助金和必要时的部门支持。该实验室有病理科和MGH管理部门的财政支持文件。相关性：这项申请向美国国立卫生研究院申请资金，以购买研究和理解免疫系统细胞如何用于治疗白血病和淋巴瘤等恶性肿瘤所必需的高科技仪器。今天，在过去类似工作的基础上取得的医学进步，使得治愈的肾脏和骨髓移植以及某些自身免疫和传染病的治疗成为可能。

项目成果

Normal and malignant muscle cell transplantation into immune compromised adult zebrafish.

• DOI: 10.3791/52597
• 发表时间: 2014-12-26
• 期刊: Journal of visualized experiments : JoVE
• 作者: Tenente IM;Tang Q;Moore JC;Langenau DM
• 通讯作者: Langenau DM

Bi-specific molecule against EGFR and death receptors simultaneously targets proliferation and death pathways in tumors.

• DOI: 10.1038/s41598-017-02483-9
• 发表时间: 2017-06-01
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Zhu Y;Bassoff N;Reinshagen C;Bhere D;Nowicki MO;Lawler SE;Roux J;Shah K
• 通讯作者: Shah K

Single-cell transcriptional analysis of normal, aberrant, and malignant hematopoiesis in zebrafish.

• DOI: 10.1084/jem.20152013
• 发表时间: 2016-05-30
• 期刊: The Journal of experimental medicine
• 作者: Moore FE;Garcia EG;Lobbardi R;Jain E;Tang Q;Moore JC;Cortes M;Molodtsov A;Kasheta M;Luo CC;Garcia AJ;Mylvaganam R;Yoder JA;Blackburn JS;Sadreyev RI;Ceol CJ;North TE;Langenau DM
• 通讯作者: Langenau DM

PHF6 Expression Levels Impact Human Hematopoietic Stem Cell Differentiation.

• DOI: 10.3389/fcell.2020.599472
• 发表时间: 2020
• 期刊: Frontiers in cell and developmental biology
• 影响因子: 5.5
• 作者: Loontiens S;Dolens AC;Strubbe S;Van de Walle I;Moore FE;Depestel L;Vanhauwaert S;Matthijssens F;Langenau DM;Speleman F;Van Vlierberghe P;Durinck K;Taghon T
• 通讯作者: Taghon T

Tumor Resection Recruits Effector T Cells and Boosts Therapeutic Efficacy of Encapsulated Stem Cells Expressing IFNβ in Glioblastomas.

• DOI: 10.1158/1078-0432.ccr-17-0077
• 发表时间: 2017-11-15
• 期刊: Clinical cancer research : an official journal of the American Association for Cancer Research
• 作者: Choi SH;Stuckey DW;Pignatta S;Reinshagen C;Khalsa JK;Roozendaal N;Martinez-Quintanilla J;Tamura K;Keles E;Shah K
• 通讯作者: Shah K