Translational Immunology Core's FACSAria Cell Sorter

项目摘要/摘要：申请从Becton-Dickinson购买一台4激光FACSAria，用于PI在转化免疫学中心（TIC）的新流式细胞仪核心，该中心将于2007年开业。TIC是马萨诸塞州总医院（Massachusetts General Hospital）查尔斯敦海军造船厂研究中心（Charlestown Navy Yard research facility）正在建设的一个新的专题研究设施。TIC将把基础科学的进展转化为实体器官和骨髓移植、炎症性肠病、肿瘤免疫学和感染性、免疫缺陷和自身免疫性疾病的临床实践。如果获得资助，所要求的仪器将作为TIC研究资源的细胞分析中心。PI在利用多色细胞术进行研究和临床工作方面有良好的记录，并有文件表明所要求的仪器将为TIC的需求提供必要的资源。该仪器最初将服务于来自5个部门的15名主要研究人员组成的多学科小组，代表67个NIH资助的拨款；它将进一步有利于位于查尔斯敦研究机构的整个研究社区，他们目前缺乏适当的细胞仪来进行NIH资助的工作。自1985年以来，PI一直为MGH研究界服务，他的实验室是MGH所有研究人员唯一可以方便使用的实验室。本实验室拥有技术娴熟的操作人员，他们与PI一起，共同代表了40多年的流式细胞术经验。实验室的管理和维持是依靠使用者收费、拨款和部门在必要时的支持。该实验室得到病理科和MGH管理部门的财政支持。相关性：本申请向美国国立卫生研究院申请资金，用于购买研究和理解免疫系统细胞如何用于治疗恶性肿瘤（如白血病和淋巴瘤）所必需的高科技仪器。基于过去类似工作的医学进步，今天允许治疗肾和骨髓移植以及某些自身免疫性疾病和传染病的治疗。

Project Summary/Abstract: Funds are requested to purchase a 4-laser FACSAria from Becton-Dickinson, to be placed in the PI's new Flow Cytometry Core in the Translational Immunology Center [TIC], opening in 2007. The TIC is a new thematic research facility underway at Massachusetts General Hospital's [MGH] Charlestown Navy Yard Research Facility. The TIC will translate advances in basic science to clinical practice in solid organ and bone marrow transplantation, inflammatory bowel disease, tumor immunology and infectious, immunodeficiency and autoimmune diseases. If funded, the requested instrument will serve as the cytometric centerpiece of the TIC's research resource. The PI has an established track record in utilizing polychromatic cytometry for research and clinical work and documents that the requested instrument will provide an essential resource to the needs of the TIC. This instrument will initially serve a multidisciplinary group of 15 primary investigators from 5 departments, representing 67 NIH funded grants; it will further benefit the entire research community located in the Charlestown Research Facility, who presently lack appropriate cytometric instrumentation for their NIH funded work. The PI has served the MGH research community since 1985, and his laboratory has been the only one easily available to all investigators at MGH. This laboratory has a highly skilled operator, who along with the PI, collectively represent well over 40 years of well documented experience in flow cytometry. The laboratory has administered and sustained itself on a combination of user fees, grants and departmental backing when necessary. The laboratory has the documented financial support of both the Department of Pathology and MGH administration. Relevance: This application petitions the NIH for funds to purchase high-technology instrumentation essential to study and understand how cells of the immune system can be used to treat malignancies such as leukemia and lymphoma. Advances in medicine based on similar past work today permit curative kidney and bone marrow transplantations as well as treatments for certain autoimmune and infectious disease.