Translational Immunology Core's FACSAria Cell Sorter

项目概要/摘要：申请资金用于从Becton-Dickinson购买4激光FACSAria，将其放置在2007年开放的转化免疫学中心[TIC]的PI新流式细胞术核心中。TIC是马萨诸塞州总医院（MGH）查尔斯敦海军造船厂研究设施正在进行的一项新的主题研究设施。TIC将把基础科学的进展转化为实体器官和骨髓移植、炎症性肠病、肿瘤免疫学以及感染性、免疫缺陷和自身免疫性疾病的临床实践。如果获得资助，所要求的仪器将作为TIC研究资源的细胞计数中心。PI在将多色细胞仪用于研究和临床工作方面具有既定的跟踪记录，并记录所需仪器将为TIC的需求提供必要的资源。该仪器最初将为来自5个部门的15名主要研究人员组成的多学科小组提供服务，代表NIH资助的67笔赠款;它将进一步使位于查尔斯敦研究机构的整个研究团体受益，他们目前缺乏适当的细胞计数仪器用于NIH资助的工作。PI自1985年以来一直为MGH研究界服务，他的实验室是MGH所有研究人员唯一容易使用的实验室。该实验室有一名技术熟练的操作员，沿着PI，共同代表了40多年的流式细胞术经验。化验所的管理和维持，是以使用费、拨款和部门资助（如有需要）为依归。实验室有病理学系和MGH管理部门的书面财政支持。相关性：该申请请求NIH提供资金，用于购买研究和了解免疫系统细胞如何用于治疗白血病和淋巴瘤等恶性肿瘤所必需的高科技仪器。基于过去类似工作的医学进步今天允许治愈性肾脏和骨髓移植以及某些自身免疫性和传染性疾病的治疗。

Project Summary/Abstract: Funds are requested to purchase a 4-laser FACSAria from Becton-Dickinson, to be placed in the PI's new Flow Cytometry Core in the Translational Immunology Center [TIC], opening in 2007. The TIC is a new thematic research facility underway at Massachusetts General Hospital's [MGH] Charlestown Navy Yard Research Facility. The TIC will translate advances in basic science to clinical practice in solid organ and bone marrow transplantation, inflammatory bowel disease, tumor immunology and infectious, immunodeficiency and autoimmune diseases. If funded, the requested instrument will serve as the cytometric centerpiece of the TIC's research resource. The PI has an established track record in utilizing polychromatic cytometry for research and clinical work and documents that the requested instrument will provide an essential resource to the needs of the TIC. This instrument will initially serve a multidisciplinary group of 15 primary investigators from 5 departments, representing 67 NIH funded grants; it will further benefit the entire research community located in the Charlestown Research Facility, who presently lack appropriate cytometric instrumentation for their NIH funded work. The PI has served the MGH research community since 1985, and his laboratory has been the only one easily available to all investigators at MGH. This laboratory has a highly skilled operator, who along with the PI, collectively represent well over 40 years of well documented experience in flow cytometry. The laboratory has administered and sustained itself on a combination of user fees, grants and departmental backing when necessary. The laboratory has the documented financial support of both the Department of Pathology and MGH administration. Relevance: This application petitions the NIH for funds to purchase high-technology instrumentation essential to study and understand how cells of the immune system can be used to treat malignancies such as leukemia and lymphoma. Advances in medicine based on similar past work today permit curative kidney and bone marrow transplantations as well as treatments for certain autoimmune and infectious disease.