Translational Immunology Core's FACSAria Cell Sorter

转化免疫学核心的 FACSAria 细胞分选仪

• 批准号: 7390038
• 负责人: FREDERIC Ira PREFFER
• 金额: $ 50万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7390038
• 关键词: Autoimmune Diseases; Autoimmune Process; Back; Basic Science; Bone Marrow Transplantation; Cells; Clinical; Communicable Diseases; Communities; Cytometry; Fees; Financial Support; Flow Cytometry; Funding; General Hospitals; Grant; Immune system; Immunologic Deficiency Syndromes; Immunology; Inflammatory Bowel Diseases; Kidney; Laboratories; Lasers; Malignant Neoplasms; Massachusetts; Medicine; Organ; Pathology; Research; Research Personnel; Resources; Solid; Technology; Today; Translating; United States National Institutes of Health; Work; abstracting; base; experience; instrument; instrumentation; leukemia/lymphoma; multidisciplinary; research facility; tumor immunology

Project Summary/Abstract: Funds are requested to purchase a 4-laser FACSAria from Becton-Dickinson, to be placed in the PI's new Flow Cytometry Core in the Translational Immunology Center [TIC], opening in 2007. The TIC is a new thematic research facility underway at Massachusetts General Hospital's [MGH] Charlestown Navy Yard Research Facility. The TIC will translate advances in basic science to clinical practice in solid organ and bone marrow transplantation, inflammatory bowel disease, tumor immunology and infectious, immunodeficiency and autoimmune diseases. If funded, the requested instrument will serve as the cytometric centerpiece of the TIC's research resource. The PI has an established track record in utilizing polychromatic cytometry for research and clinical work and documents that the requested instrument will provide an essential resource to the needs of the TIC. This instrument will initially serve a multidisciplinary group of 15 primary investigators from 5 departments, representing 67 NIH funded grants; it will further benefit the entire research community located in the Charlestown Research Facility, who presently lack appropriate cytometric instrumentation for their NIH funded work. The PI has served the MGH research community since 1985, and his laboratory has been the only one easily available to all investigators at MGH. This laboratory has a highly skilled operator, who along with the PI, collectively represent well over 40 years of well documented experience in flow cytometry. The laboratory has administered and sustained itself on a combination of user fees, grants and departmental backing when necessary. The laboratory has the documented financial support of both the Department of Pathology and MGH administration. Relevance: This application petitions the NIH for funds to purchase high-technology instrumentation essential to study and understand how cells of the immune system can be used to treat malignancies such as leukemia and lymphoma. Advances in medicine based on similar past work today permit curative kidney and bone marrow transplantations as well as treatments for certain autoimmune and infectious disease.

项目摘要/摘要：需要资金从 Becton-Dickinson 购买 4 激光 FACSAria，并将其放置在 PI 于 2007 年开放的转化免疫学中心 [TIC] 的新流式细胞术核心中。TIC 是马萨诸塞州总医院 [MGH] 查尔斯敦海军造船厂研究设施中正在建设的一个新的主题研究设施。 TIC 将把基础科学的进步转化为实体器官和骨髓移植、炎症性肠病、肿瘤免疫学以及传染病、免疫缺陷和自身免疫性疾病的临床实践。如果获得资助，所请求的仪器将作为 TIC 研究资源的细胞计数核心。 PI 在利用多色细胞术进行研究和临床工作方面拥有良好的记录，并证明所需的仪器将为满足 TIC 的需求提供重要资源。该工具最初将为来自 5 个部门的 15 名主要研究人员组成的多学科小组提供服务，代表 67 项 NIH 资助的赠款；它将进一步使位于查尔斯敦研究机构的整个研究界受益，他们目前缺乏适当的细胞计数仪器来进行 NIH 资助的工作。该 PI 自 1985 年以来一直为 MGH 研究界服务，他的实验室是 MGH 唯一一个可供所有研究人员轻松使用的实验室。该实验室拥有一名技术精湛的操作员，他们与 PI 一起在流式细胞术领域拥有 40 多年的丰富经验。该实验室通过使用费、赠款和必要时部门支持的结合来管理和维持自身。该实验室得到了病理科和麻省总医院管理部门的有据可查的财政支持。相关性：本申请向 NIH 申请资金购买高科技仪器，这些仪器对于研究和了解免疫系统细胞如何用于治疗白血病和淋巴瘤等恶性肿瘤至关重要。基于过去类似工作的医学进步如今允许进行治疗性肾脏和骨髓移植以及某些自身免疫性疾病和传染病的治疗。

项目成果

Normal and malignant muscle cell transplantation into immune compromised adult zebrafish.

• DOI: 10.3791/52597
• 发表时间: 2014-12-26
• 期刊: Journal of visualized experiments : JoVE
• 作者: Tenente IM;Tang Q;Moore JC;Langenau DM
• 通讯作者: Langenau DM

Bi-specific molecule against EGFR and death receptors simultaneously targets proliferation and death pathways in tumors.

• DOI: 10.1038/s41598-017-02483-9
• 发表时间: 2017-06-01
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Zhu Y;Bassoff N;Reinshagen C;Bhere D;Nowicki MO;Lawler SE;Roux J;Shah K
• 通讯作者: Shah K

Single-cell transcriptional analysis of normal, aberrant, and malignant hematopoiesis in zebrafish.

• DOI: 10.1084/jem.20152013
• 发表时间: 2016-05-30
• 期刊: The Journal of experimental medicine
• 作者: Moore FE;Garcia EG;Lobbardi R;Jain E;Tang Q;Moore JC;Cortes M;Molodtsov A;Kasheta M;Luo CC;Garcia AJ;Mylvaganam R;Yoder JA;Blackburn JS;Sadreyev RI;Ceol CJ;North TE;Langenau DM
• 通讯作者: Langenau DM

PHF6 Expression Levels Impact Human Hematopoietic Stem Cell Differentiation.

• DOI: 10.3389/fcell.2020.599472
• 发表时间: 2020
• 期刊: Frontiers in cell and developmental biology
• 影响因子: 5.5
• 作者: Loontiens S;Dolens AC;Strubbe S;Van de Walle I;Moore FE;Depestel L;Vanhauwaert S;Matthijssens F;Langenau DM;Speleman F;Van Vlierberghe P;Durinck K;Taghon T
• 通讯作者: Taghon T

Tumor Resection Recruits Effector T Cells and Boosts Therapeutic Efficacy of Encapsulated Stem Cells Expressing IFNβ in Glioblastomas.

• DOI: 10.1158/1078-0432.ccr-17-0077
• 发表时间: 2017-11-15
• 期刊: Clinical cancer research : an official journal of the American Association for Cancer Research
• 作者: Choi SH;Stuckey DW;Pignatta S;Reinshagen C;Khalsa JK;Roozendaal N;Martinez-Quintanilla J;Tamura K;Keles E;Shah K
• 通讯作者: Shah K