Department of Pathology LSR2 Cytometer

描述（由申请人提供）：申请资金用于购买一台特殊的多激光LSR-2流式细胞仪[Becton-Dickinson]，该仪器将放置在将于2005年开放的再生医学与技术中心（CRMT） PI的新流式细胞仪核心中。CMRT是耗资1.5亿美元的全新研究大楼的一部分，该大楼目前正在波士顿市中心建设，是马萨诸塞州总医院（MGH）的一部分。普雷弗博士的核心细胞术实验室将是整个新研究大楼或毗邻的MGH中唯一的一个。如果获得资助，所要求的仪器将作为集成的5激光FACSDiVa细胞分选系统的分析核心。特殊多激光LSR-2是一个六激光仪器，PI不知道任何商业上可用的流式细胞仪在大波士顿地区类似的。PI最近展示了十色免疫荧光能力，并认为所要求的仪器将进一步实现该应用中指定的共同研究者的目标。由于近年来细胞术的巨大改进和技术进步，PI现有的LSR-1在技术上无法达到所要求仪器固有的性能水平。病理学系流式细胞术核心实验室自1978年以来一直服务于MGH研究界，是MGH所有研究人员唯一可以方便使用的实验室。对于这项申请，所要求的仪器将服务于一个由18个pi组成的多学科小组，代表24个NIH资助的拨款。该实验室拥有高度熟练的操作员和PI，在流式细胞术方面有40年的丰富经验。实验室的管理和维持是依靠使用者收费、拨款和部门在必要时的支持。该实验室得到病理科和MGH管理部门的财政支持。

DESCRIPTION (provided by applicant): Funds are requested to purchase a Special Multi-Laser LSR-2 flow cytometer [Becton-Dickinson] to be placed in the PI's new Flow Cytometry Core in the Center for Regenerative Medicine and Technology [CRMT] opening in 2005. The CMRT is one part of an entirely new 150 million-dollar research building presently under construction in downtown Boston, part of Massachusetts General Hospital [MGH]. Dr. Preffer's Core Cytometry Laboratory will be the only one in the entire new research building or the adjoining MGH. If funded, the requested instrument will serve as the analytic centerpiece to an integrated 5-laser FACSDiVa cell sorting system. The Special Multi-Laser LSR-2 is a six laser instrument and the PI is unaware of any commercially available flow cytometer in the greater metropolitan Boston area similar to it. The PI has recently demonstrated ten-color immunofluorescence capacity and feels the requested instrument will further the aims of the named co-investigators in this application. Due to vast improvements and technological advances in cytometry in recent years, the PI's existing LSR-1 is technologically incapable of the level of performance inherent in the requested instrument. The Department of Pathology Flow Cytometry Core laboratory has served the MGH research community since 1978, and is the only one easily available to all investigators at MGH. For this application, the requested instrument will serve a multidisciplinary group of 18 PIs representing 24 NIH funded grants. The laboratory has a highly skilled operator and PI with a combined 40 years of well documented experience in flow cytometry. The laboratory has administered and sustained itself on a combination of user fees, grants and departmental backing when necessary. The laboratory has the documented financial support of both the Department of Pathology and MGH administration.