Department of Pathology LSR2 Cytometer

描述(由申请人提供)：请提供资金购买一台特殊的多激光LSR-2流式细胞仪[Becton-Dickinson]，以放置在2005年开放的再生医学与技术中心PI的新的流式细胞仪核心中。CMRT是波士顿市中心一座耗资1.5亿美元的全新研究建筑的一部分，波士顿市中心是马萨诸塞州综合医院(MGH)的一部分。普雷弗博士的核心细胞学实验室将是整个新研究大楼或毗邻的MGH中唯一的一个。如果获得资金，所要求的仪器将作为集成的5激光FACSDiVa细胞分选系统的分析中心。特殊的多激光LSR-2是一种六激光仪器，PI不知道在大都市波士顿地区有任何商业上可用的流式细胞仪与它类似。PI最近展示了十色免疫荧光能力，并认为所要求的仪器将促进指定的协查人员在这一应用中的目标。由于近年来细胞学的巨大改进和技术进步，PI现有的LSR-1在技术上无法达到所要求的仪器固有的性能水平。病理学系流式细胞术核心实验室自1978年以来一直服务于MGH研究社区，是MGH所有研究人员都可以轻松获得的唯一实验室。对于这项申请，所要求的工具将为一个由18个私人投资机构组成的多学科小组提供服务，这些机构代表着国家卫生研究院资助的24笔赠款。该实验室拥有一名技术精湛的操作员和PI，并在流式细胞仪领域拥有40年有据可查的经验。实验室的管理和维持依靠的是使用费、补助金和必要时的部门支持。该实验室有病理科和MGH管理部门的财政支持文件。

DESCRIPTION (provided by applicant): Funds are requested to purchase a Special Multi-Laser LSR-2 flow cytometer [Becton-Dickinson] to be placed in the PI's new Flow Cytometry Core in the Center for Regenerative Medicine and Technology [CRMT] opening in 2005. The CMRT is one part of an entirely new 150 million-dollar research building presently under construction in downtown Boston, part of Massachusetts General Hospital [MGH]. Dr. Preffer's Core Cytometry Laboratory will be the only one in the entire new research building or the adjoining MGH. If funded, the requested instrument will serve as the analytic centerpiece to an integrated 5-laser FACSDiVa cell sorting system. The Special Multi-Laser LSR-2 is a six laser instrument and the PI is unaware of any commercially available flow cytometer in the greater metropolitan Boston area similar to it. The PI has recently demonstrated ten-color immunofluorescence capacity and feels the requested instrument will further the aims of the named co-investigators in this application. Due to vast improvements and technological advances in cytometry in recent years, the PI's existing LSR-1 is technologically incapable of the level of performance inherent in the requested instrument. The Department of Pathology Flow Cytometry Core laboratory has served the MGH research community since 1978, and is the only one easily available to all investigators at MGH. For this application, the requested instrument will serve a multidisciplinary group of 18 PIs representing 24 NIH funded grants. The laboratory has a highly skilled operator and PI with a combined 40 years of well documented experience in flow cytometry. The laboratory has administered and sustained itself on a combination of user fees, grants and departmental backing when necessary. The laboratory has the documented financial support of both the Department of Pathology and MGH administration.