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Department of Pathology LSR2 Cytometer

病理科LSR2细胞仪

基本信息

批准号：
6877558
负责人：
FREDERIC Ira PREFFER
金额：
$ 38.9万
依托单位：
MASSACHUSETTS GENERAL HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-01-01 至 2005-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6877558
关键词：
biomedical equipment purchase cell biology cell differentiation cell sorting flow cytometry

项目摘要

DESCRIPTION (provided by applicant): Funds are requested to purchase a Special Multi-Laser LSR-2 flow cytometer [Becton-Dickinson] to be placed in the PI's new Flow Cytometry Core in the Center for Regenerative Medicine and Technology [CRMT] opening in 2005. The CMRT is one part of an entirely new 150 million-dollar research building presently under construction in downtown Boston, part of Massachusetts General Hospital [MGH]. Dr. Preffer's Core Cytometry Laboratory will be the only one in the entire new research building or the adjoining MGH. If funded, the requested instrument will serve as the analytic centerpiece to an integrated 5-laser FACSDiVa cell sorting system. The Special Multi-Laser LSR-2 is a six laser instrument and the PI is unaware of any commercially available flow cytometer in the greater metropolitan Boston area similar to it. The PI has recently demonstrated ten-color immunofluorescence capacity and feels the requested instrument will further the aims of the named co-investigators in this application. Due to vast improvements and technological advances in cytometry in recent years, the PI's existing LSR-1 is technologically incapable of the level of performance inherent in the requested instrument. The Department of Pathology Flow Cytometry Core laboratory has served the MGH research community since 1978, and is the only one easily available to all investigators at MGH. For this application, the requested instrument will serve a multidisciplinary group of 18 PIs representing 24 NIH funded grants. The laboratory has a highly skilled operator and PI with a combined 40 years of well documented experience in flow cytometry. The laboratory has administered and sustained itself on a combination of user fees, grants and departmental backing when necessary. The laboratory has the documented financial support of both the Department of Pathology and MGH administration.

描述（由申请人提供）：申请资金用于购买一台特殊的多激光LSR-2流式细胞仪[Becton-Dickinson]，该流式细胞仪将放置在2005年开放的再生医学和技术中心[CRMT]的PI新流式细胞仪核心中。CMRT是目前正在波士顿市中心建造的一座全新的1.5亿美元研究大楼的一部分，该大楼是马萨诸塞州总医院[MGH]的一部分。Preffer博士的核心细胞计数实验室将是整个新研究大楼或毗邻的MGH中唯一的一个。如果获得资助，所要求的仪器将作为集成的5激光FACSDiVa细胞分选系统的分析中心。Special Multi-Laser LSR-2是一种六激光仪器，PI不知道波士顿大都市区有任何类似的市售流式细胞仪。PI最近证明了十色免疫荧光能力，并认为所要求的仪器将促进指定合作研究者在本申请中的目标。由于近年来细胞仪的巨大改进和技术进步，PI现有的LSR-1在技术上无法达到所需仪器的固有性能水平。病理流式细胞术核心实验室的部门自1978年以来一直服务于MGH研究界，是唯一一个容易在MGH所有研究人员。对于此应用程序，所要求的工具将服务于一个多学科组的18 PI代表24 NIH资助的赠款。该实验室拥有一名高技能的操作员和PI，他们在流式细胞术领域拥有40年的丰富经验。化验所的管理和维持，是以使用费、赠款和部门资助（如有需要）为依归。实验室有病理学系和MGH管理部门的书面财政支持。