FLOW CYTOMETRY CORE
流式细胞术核心
基本信息
- 批准号:7158103
- 负责人:
- 金额:$ 3.55万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2006
- 资助国家:美国
- 起止时间:2006-07-01 至 2011-06-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Core A contains a Becton-Dickinson DiVa cell sorter and LSR analyzer. The DiVa is a 13-parameter 4-way
5-laser cell sorter. The LSR has excitation lines in the ultraviolet, visible and red spectra. The Core Leader
was recently awarded (1/1/05) a SIG to build a 6-laser, 20-detector LSR-2 which will be available for this
program. For Project 1 (Sykes), Core A will be used for 9-color flow cytometry studies required to assess
activation and memory markers on GVH-reactive and non-reactive T cell subsets identified with specific
markers, for CFSE studies, and for sorting of T cell subsets to be adoptively transferred from lymphoid and
non-lymphoid tissues of GVHD mice and delayed DLI recipients. Project 2 (Yang) will investigate the
mechanisms by which IFN-gamma separates GVL effects from GVHD-inducing activity of donor CDS T cells
in allogeneic HCT recipients. The program will need a cell sorter about 2 days/month to purify donor from
recipient mouse cells, obtained from approximately 30 mice/yr, for transplantation. For Project 3 (Sachs),
Core A will be used for the specific T-cell subset depletion of the donor DLI inoculum and the separation of
donor-type and host-type T-cells from PBMC of chimeric recipients at monthly intervals, following HCT for
the purpose of in vitro and in vivo mechanistic studies of regulatory cells. For Project 4 (Scadden), the result
of PTH administration on CD34+ cell yields in patients treated with G-CSF mobilization will be monitored.
The immune reconstitution of post-transplant patients treated with PTH will also be monitored. For the
murine studies related to Project 4, Core A will assess and separate lymphocytes based upon T-cell
differentiation antigens and MHC specificity. Approximately 20 sorts and 45 analyses will be needed for this
project.
Core A包含一个Becton-Dickinson DiVa细胞分选器和LSR分析仪。DiVa是一个13参数4路
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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FREDERIC Ira PREFFER其他文献
FREDERIC Ira PREFFER的其他文献
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{{ truncateString('FREDERIC Ira PREFFER', 18)}}的其他基金
Translational Immunology Core's FACSAria Cell Sorter
转化免疫学核心的 FACSAria 细胞分选仪
- 批准号:
7390038 - 财政年份:2008
- 资助金额:
$ 3.55万 - 项目类别:
DEPARTMENT OF PATHOLOGY LSR2 CYTOMETER: TRANSPLANTATION
病理科 LSR2 细胞仪:移植
- 批准号:
7166392 - 财政年份:2005
- 资助金额:
$ 3.55万 - 项目类别:
DEPARTMENT OF PATHOLOGY LSR2 CYTOMETER: CELL BIOLOGY
病理学系 LSR2 细胞仪:细胞生物学
- 批准号:
7166393 - 财政年份:2005
- 资助金额:
$ 3.55万 - 项目类别:
DEPARTMENT OF PATHOLOGY LSR2 CYTOMETER: DIABETES
病理学部 LSR2 细胞仪:糖尿病
- 批准号:
7166395 - 财政年份:2005
- 资助金额:
$ 3.55万 - 项目类别:
DEPARTMENT OF PATHOLOGY LSR2 CYTOMETER: ADULT ANIMAL STEM CELLS
病理学系 LSR2 细胞仪:成年动物干细胞
- 批准号:
7166394 - 财政年份:2005
- 资助金额:
$ 3.55万 - 项目类别:
DEPARTMENT OF PATHOLOGY - HIGH SPEED CELL SORTER
病理科 - 高速细胞分选机
- 批准号:
6054241 - 财政年份:2000
- 资助金额:
$ 3.55万 - 项目类别:
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