FLOW CYTOMETRY CORE

流式细胞术核心

基本信息

  • 批准号:
    7158103
  • 负责人:
  • 金额:
    $ 3.55万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2006
  • 资助国家:
    美国
  • 起止时间:
    2006-07-01 至 2011-06-30
  • 项目状态:
    已结题

项目摘要

Core A contains a Becton-Dickinson DiVa cell sorter and LSR analyzer. The DiVa is a 13-parameter 4-way 5-laser cell sorter. The LSR has excitation lines in the ultraviolet, visible and red spectra. The Core Leader was recently awarded (1/1/05) a SIG to build a 6-laser, 20-detector LSR-2 which will be available for this program. For Project 1 (Sykes), Core A will be used for 9-color flow cytometry studies required to assess activation and memory markers on GVH-reactive and non-reactive T cell subsets identified with specific markers, for CFSE studies, and for sorting of T cell subsets to be adoptively transferred from lymphoid and non-lymphoid tissues of GVHD mice and delayed DLI recipients. Project 2 (Yang) will investigate the mechanisms by which IFN-gamma separates GVL effects from GVHD-inducing activity of donor CDS T cells in allogeneic HCT recipients. The program will need a cell sorter about 2 days/month to purify donor from recipient mouse cells, obtained from approximately 30 mice/yr, for transplantation. For Project 3 (Sachs), Core A will be used for the specific T-cell subset depletion of the donor DLI inoculum and the separation of donor-type and host-type T-cells from PBMC of chimeric recipients at monthly intervals, following HCT for the purpose of in vitro and in vivo mechanistic studies of regulatory cells. For Project 4 (Scadden), the result of PTH administration on CD34+ cell yields in patients treated with G-CSF mobilization will be monitored. The immune reconstitution of post-transplant patients treated with PTH will also be monitored. For the murine studies related to Project 4, Core A will assess and separate lymphocytes based upon T-cell differentiation antigens and MHC specificity. Approximately 20 sorts and 45 analyses will be needed for this project.
Core A包含一个Becton-Dickinson DiVa细胞分选器和LSR分析仪。DiVa是一个13参数4路

项目成果

期刊论文数量(0)
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会议论文数量(0)
专利数量(0)

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FREDERIC Ira PREFFER其他文献

FREDERIC Ira PREFFER的其他文献

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{{ truncateString('FREDERIC Ira PREFFER', 18)}}的其他基金

Influx Flow Cytometer
流入流式细胞仪
  • 批准号:
    8447644
  • 财政年份:
    2013
  • 资助金额:
    $ 3.55万
  • 项目类别:
ImageStream X Analyzer 2011
ImageStream X 分析仪 2011
  • 批准号:
    8247324
  • 财政年份:
    2012
  • 资助金额:
    $ 3.55万
  • 项目类别:
Translational Immunology Core's FACSAria Cell Sorter
转化免疫学核心的 FACSAria 细胞分选仪
  • 批准号:
    7390038
  • 财政年份:
    2008
  • 资助金额:
    $ 3.55万
  • 项目类别:
DEPARTMENT OF PATHOLOGY LSR2 CYTOMETER: AIDS
病理科 LSR2 细胞仪:艾滋病
  • 批准号:
    7166391
  • 财政年份:
    2005
  • 资助金额:
    $ 3.55万
  • 项目类别:
Department of Pathology LSR2 Cytometer
病理科LSR2细胞仪
  • 批准号:
    6877558
  • 财政年份:
    2005
  • 资助金额:
    $ 3.55万
  • 项目类别:
DEPARTMENT OF PATHOLOGY LSR2 CYTOMETER: TRANSPLANTATION
病理科 LSR2 细胞仪:移植
  • 批准号:
    7166392
  • 财政年份:
    2005
  • 资助金额:
    $ 3.55万
  • 项目类别:
DEPARTMENT OF PATHOLOGY LSR2 CYTOMETER: CELL BIOLOGY
病理学系 LSR2 细胞仪:细胞生物学
  • 批准号:
    7166393
  • 财政年份:
    2005
  • 资助金额:
    $ 3.55万
  • 项目类别:
DEPARTMENT OF PATHOLOGY LSR2 CYTOMETER: DIABETES
病理学部 LSR2 细胞仪:糖尿病
  • 批准号:
    7166395
  • 财政年份:
    2005
  • 资助金额:
    $ 3.55万
  • 项目类别:
DEPARTMENT OF PATHOLOGY LSR2 CYTOMETER: ADULT ANIMAL STEM CELLS
病理学系 LSR2 细胞仪:成年动物干细胞
  • 批准号:
    7166394
  • 财政年份:
    2005
  • 资助金额:
    $ 3.55万
  • 项目类别:
DEPARTMENT OF PATHOLOGY - HIGH SPEED CELL SORTER
病理科 - 高速细胞分选机
  • 批准号:
    6054241
  • 财政年份:
    2000
  • 资助金额:
    $ 3.55万
  • 项目类别:

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职业:下一代流式细胞术 - 细胞异质性的新方法
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Omics, Bioinformatics and Flow Cytometry Core
组学、生物信息学和流式细胞术核心
  • 批准号:
    10630580
  • 财政年份:
    2023
  • 资助金额:
    $ 3.55万
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Flow Cytometry Core
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    10630389
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High-parametric flow cytometry cell sorter for human immunology studies.
用于人类免疫学研究的高参数流式细胞仪细胞分选仪。
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用于 T 细胞功能动力学分析的延时流式细胞术
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以细胞周期为中心的流式细胞术诊断恶性胸膜间皮瘤胸腔积液的方法的建立
  • 批准号:
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用于索尔克研究所流式细胞术核心的光谱配置 Bigfoot 分选机
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