DEPARTMENT OF PATHOLOGY - HIGH SPEED CELL SORTER

申请资金用于购买一种新的Cytomation MLS高速细胞软器，以取代已有15年历史的EPICS 753细胞分选器。自1978年以来，麻省总医院（MGH）病理科的流式细胞术核心一直是向所有需要流式细胞术的基础和临床研究科学家开放的持续资源。该实验室的管理和维持是依靠用户费用和研究补助金相结合的方式，必要时还会得到部门的支持。该实验室有一个高度熟练的操作员和PI在流式细胞术记录的专业知识，导致合并30年的流式细胞术经验。需要当代细胞分选器来维持和改进27个正在进行的NIH资助项目，这些项目由10个主要和6个次要主要研究人员组成，在本申请中描述。与EPICS 753相比，所要求的仪器的规格保守地预测分选速度总体提高十倍，多色分析能力大大提高。本提案的目的是说明如何EPICS 753，这是在20世纪70年代后期设计的，已充分利用其最大容量，但无法达到当前最先进的仪器的性能水平。此外，库尔特-贝克曼公司不再为EPICS 753提供服务。委员会认为，为新文书所要求的备选办法是合理的，并得到提供资料的用户团体提交的项目摘要的支持

Funding is requested to purchase a new Cytomation MLS high speed cell softer to replace a 15-year-old EPICS 753 cell sorter. The Flow Cytometry Core in the Department Of Pathology at Massachusetts General Hospital (MGH) has been a continuous resource open to all basic and clinical research scientists requiring flow cytometry since 1978. The laboratory has administered and sustained itself on a combination of user fees and research grants, with departmental backing when necessary. The laboratory has a highly skilled operator and PI with documented expertise in flow cytometry, resulting in a combined 30 years of experience in flow cytometry. The need for a contemporary cell sorter is required to sustain and improve 27 ongoing NIH funded projects from combination of 10 Major and 6 Minor Principal Investigators, described in this application. The requested instrument has specifications that conservatively project an overall tenfold improvement in sorting speed and greatly improved multicolor analytic capacity, compared to the EPICS 753. The purpose of this proposal is to illustrate how the EPICS 753, which was engineered in the late 1970's, has been thoroughly utilized to its utmost capacity but is unable to attain the performance level of the current state-of- the-art instrumentation. Furthermore, Coulter-Beckman no longer services the EPICS 753. It is felt that the options requested for the new instrument are reasonable and warranted by the project summaries submitted by the contributing user groups