DEPARTMENT OF PATHOLOGY - HIGH SPEED CELL SORTER

申请资金用于购买一台新的Cytomation MLS高速细胞分选仪，以取代使用了15年的EPICS 753细胞分选仪。自1978年以来，马萨诸塞州总医院（MGH）病理科的流式细胞术核心一直是向所有需要流式细胞术的基础和临床研究科学家开放的连续资源。实验室的管理和维持，是以使用费和研究赠款为基础，并在有需要时得到部门的支持。该实验室拥有一名高技能的操作员和PI，他们在流式细胞术方面拥有记录在案的专业知识，因此在流式细胞术方面拥有30年的经验。需要当代细胞分选机来维持和改进本申请中描述的来自10名主要和6名次要主要研究者的27个正在进行的NIH资助项目。与EPICS 753相比，所要求的仪器具有保守预测分选速度整体提高十倍的规格，并大大提高了分析能力。本提案的目的是说明EPICS 753是如何在20世纪70年代末设计的，已被充分利用到其最大容量，但无法达到当前最先进仪器的性能水平。此外，Coulter-Beckman不再为EPICS 753提供服务。人们认为，为新文书要求的备选方案是合理的，并得到了贡献用户团体提交的项目摘要的支持

Funding is requested to purchase a new Cytomation MLS high speed cell softer to replace a 15-year-old EPICS 753 cell sorter. The Flow Cytometry Core in the Department Of Pathology at Massachusetts General Hospital (MGH) has been a continuous resource open to all basic and clinical research scientists requiring flow cytometry since 1978. The laboratory has administered and sustained itself on a combination of user fees and research grants, with departmental backing when necessary. The laboratory has a highly skilled operator and PI with documented expertise in flow cytometry, resulting in a combined 30 years of experience in flow cytometry. The need for a contemporary cell sorter is required to sustain and improve 27 ongoing NIH funded projects from combination of 10 Major and 6 Minor Principal Investigators, described in this application. The requested instrument has specifications that conservatively project an overall tenfold improvement in sorting speed and greatly improved multicolor analytic capacity, compared to the EPICS 753. The purpose of this proposal is to illustrate how the EPICS 753, which was engineered in the late 1970's, has been thoroughly utilized to its utmost capacity but is unable to attain the performance level of the current state-of- the-art instrumentation. Furthermore, Coulter-Beckman no longer services the EPICS 753. It is felt that the options requested for the new instrument are reasonable and warranted by the project summaries submitted by the contributing user groups