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DEPARTMENT OF PATHOLOGY - HIGH SPEED CELL SORTER

病理科 - 高速细胞分选机

基本信息

批准号：
6054241
负责人：
FREDERIC Ira PREFFER
金额：
$ 47.28万
依托单位：
MASSACHUSETTS GENERAL HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2000
资助国家：
美国
起止时间：
2000-04-01 至 2001-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6054241
关键词：
biomedical equipment purchase flow cytometry

项目摘要

Funding is requested to purchase a new Cytomation MLS high speed cell softer to replace a 15-year-old EPICS 753 cell sorter. The Flow Cytometry Core in the Department Of Pathology at Massachusetts General Hospital (MGH) has been a continuous resource open to all basic and clinical research scientists requiring flow cytometry since 1978. The laboratory has administered and sustained itself on a combination of user fees and research grants, with departmental backing when necessary. The laboratory has a highly skilled operator and PI with documented expertise in flow cytometry, resulting in a combined 30 years of experience in flow cytometry. The need for a contemporary cell sorter is required to sustain and improve 27 ongoing NIH funded projects from combination of 10 Major and 6 Minor Principal Investigators, described in this application. The requested instrument has specifications that conservatively project an overall tenfold improvement in sorting speed and greatly improved multicolor analytic capacity, compared to the EPICS 753. The purpose of this proposal is to illustrate how the EPICS 753, which was engineered in the late 1970's, has been thoroughly utilized to its utmost capacity but is unable to attain the performance level of the current state-of- the-art instrumentation. Furthermore, Coulter-Beckman no longer services the EPICS 753. It is felt that the options requested for the new instrument are reasonable and warranted by the project summaries submitted by the contributing user groups

需要资金购买新的Cytomation MLS高速细胞软化器，以取代已有15年历史的EPICS 753细胞分选机。自1978年以来，马萨诸塞州总医院(MGH)病理科的流式细胞术核心一直向所有需要流式细胞术的基础和临床研究科学家开放。该实验室依靠使用费和研究补助金进行管理和维持，必要时还会得到部门的支持。该实验室拥有一名技术精湛的操作员和PI，他们在流式细胞仪方面拥有有据可查的专业知识，从而在流式细胞仪领域积累了30年的经验。需要一台现代细胞分选机，以维持和改进NIH资助的27个正在进行的项目，这些项目由10名主要调查人员和6名次要首席调查人员组成，如本申请所述。与EPICS 753相比，所要求的仪器的规格保守地预计分拣速度总体上会提高10倍，多色分析能力也会大大提高。这项建议的目的是说明EPICS753，这是在1970年代末设计的S，如何充分利用其最大能力，但无法达到目前最先进的乐器的性能水平。此外，库尔特-贝克曼不再为史诗753提供服务。人们认为，为新文书要求的备选方案是合理的，而且由提供资料的用户团体提交的项目摘要是有根据的