ImageStream X Analyzer 2011

ImageStream X 分析仪 2011

• 批准号: 8247324
• 负责人: FREDERIC Ira PREFFER
• 金额: $ 59.5万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-01 至 2013-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8247324
• 关键词: Area; Autoimmune Process; Biological Assay; Boston; Budgets; Cells; Cellular biology; Communicable Diseases; Confocal Microscopy; Data; Dyes; Flow Cytometry; Fluorescence Microscopy; Funding; General Hospitals; Geographic Locations; Grant; Healthcare; Healthcare Systems; Hospitals; Image; Imagery; Immunofluorescence Immunologic; Immunology; Immunophenotyping; Investigation; Laboratories; Lasers; Location; Massachusetts; Microscopy; National Center for Research Resources; Pathology; Population; Principal Investigator; Research; Research Personnel; Sampling; Speed; Stem Cell Research; Technology; United States National Institutes of Health; Universities; Update; Visual; Work; base; cellular imaging; improved; insight; instrument; instrumentation; lens; oncology; operation

DESCRIPTION (provided by applicant): NIH NCRR funds are requested in this resubmission to purchase a 5-laser 2-camera Amnis ImageStreamX with three (20x, 40x, 60x) objective lenses. This new platform is a vastly improved, refined and far more powerful version of the original instrument Amnis introduced approximately eleven years ago. This institutionally supported and expertly supervised instrument will be placed in the principal investigator's existing Department of Pathology Flow Cytometry Core which has been in successful operation for almost 30 years. The requested instrument will serve to fill the gap between the highly detailed (but far more slowly acquired) cellular images obtained by confocal microscopy and the imageless but rapidly acquired data obtained by immunofluorescence-based multiparameter flow cytometry. The ImageStreamX combines these two later technologies, providing a synergistic advantage over each used in tandem. The requested instrument quantifies both the intensity and intracellular location of fluorescent cellular probes and can image at high rates of speed (up to ~2000 cells/sec), allowing our consortium of Massachusetts General Hospital (MGH) investigators to analyze rare sub- populations and highly heterogeneous samples with statistically robust and objective results. This technology will permit some investigations that would otherwise be impossible to perform, to actually be implemented. By combining the speed, sensitivity, and immunophenotyping abilities and capacities of flow cytometry with the detailed imagery and functional insights of microscopy, the requested instrument will be an invaluable addition to represented researchers at both downtown Boston and Charlestown research campuses of MGH, and throughout the greater Partners Health Care System. The instrument utilizes the same dyes and markers employed in fluorescence microscopy and flow cytometry and can perform virtually any standard flow cytometry assay with the added value of quantitative visual inspection and resulting confirmation. The requested instrument will enable 30 investigators representing 77 NIH funded grants to enter into entirely new and useful areas of investigation. There is currently no similar instrumentation at MGH, anywhere in Partners Health Care/Harvard University, or the surrounding geographic region. A wide variety of studies related to cell biology, immunology, autoimmune, infectious disease, oncology and stem cell research will benefit from the placement of this instrumentation in the principal investigator's laboratory. An ImageStreamX demo-unit was placed in the principal investigator's laboratory in February 2010 for one week; pilot data produced from those 5-days is presented in this submission as well as updated data for this 2011 re-submission. The co-investigators in this application represent over 27 million dollars of NIH funded work based at MGH; the entire hospital of approximately 1000 principal investigator's has a NIH budget of approximately $325 million dollars.

描述（由申请人提供）：在本次重新提交中要求NIH NCRR资金用于购买带有三个（20 x，40 x，60 x）物镜的5激光2相机Amnis ImageStreamX。这个新的平台是一个大大改进，完善和更强大的版本的原始仪器Amnis介绍了大约11年前。该机构支持和专家监督的仪器将放置在主要研究者现有的病理学流式细胞术核心部门，该部门已成功运行近30年。所要求的仪器将用于填补高度详细的（但更缓慢地获得）通过共聚焦显微镜获得的细胞图像和无图像，但快速获得的数据通过免疫荧光为基础的多参数流式细胞术之间的差距。ImageStreamX结合了这两种技术，提供了协同优势。所要求的仪器可定量荧光细胞探针的强度和细胞内位置，并可高速成像（高达约2000个细胞/秒），使我们的马萨诸塞州综合医院（MGH）研究者联盟能够分析罕见的亚群和高度异质性的样本，并获得统计学上稳健和客观的结果。这项技术将允许一些调查，否则将不可能执行，实际上是实施。通过结合速度，灵敏度和免疫分型能力和流式细胞术的详细图像和显微镜的功能见解的能力，所要求的仪器将是一个宝贵的除了代表研究人员在波士顿市中心和查尔斯敦研究校园的MGH，并在整个更大的合作伙伴医疗保健系统。该仪器使用荧光显微镜和流式细胞术中使用的相同染料和标记物，几乎可以执行任何标准流式细胞术分析，并具有定量目视检查和结果确认的附加值。所要求的工具将使代表77个NIH资助赠款的30名研究人员能够进入全新的有用的研究领域。目前在MGH、Partners Health Care/哈佛大学或周围地理区域的任何地方都没有类似的仪器。与细胞生物学、免疫学、自身免疫、传染病、肿瘤学和干细胞研究相关的各种研究将受益于在主要研究者实验室中放置该仪器。 2010年2月，将ImageStreamX演示装置放置在主要研究者的实验室中一周;这5天产生的试验数据见本提交资料以及2011年重新提交资料的更新数据。本申请中的合作研究者代表了MGH超过2700万美元的NIH资助工作;整个医院约1000名主要研究者的NIH预算约为3.25亿美元。