ImageStream X Analyzer 2011

ImageStream X 分析仪 2011

• 批准号: 8247324
• 负责人: FREDERIC Ira PREFFER
• 金额: $ 59.5万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-01 至 2013-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8247324
• 关键词: Area; Autoimmune Process; Biological Assay; Boston; Budgets; Cells; Cellular biology; Communicable Diseases; Confocal Microscopy; Data; Dyes; Flow Cytometry; Fluorescence Microscopy; Funding; General Hospitals; Geographic Locations; Grant; Healthcare; Healthcare Systems; Hospitals; Image; Imagery; Immunofluorescence Immunologic; Immunology; Immunophenotyping; Investigation; Laboratories; Lasers; Location; Massachusetts; Microscopy; National Center for Research Resources; Pathology; Population; Principal Investigator; Research; Research Personnel; Sampling; Speed; Stem Cell Research; Technology; United States National Institutes of Health; Universities; Update; Visual; Work; base; cellular imaging; improved; insight; instrument; instrumentation; lens; oncology; operation

DESCRIPTION (provided by applicant): NIH NCRR funds are requested in this resubmission to purchase a 5-laser 2-camera Amnis ImageStreamX with three (20x, 40x, 60x) objective lenses. This new platform is a vastly improved, refined and far more powerful version of the original instrument Amnis introduced approximately eleven years ago. This institutionally supported and expertly supervised instrument will be placed in the principal investigator's existing Department of Pathology Flow Cytometry Core which has been in successful operation for almost 30 years. The requested instrument will serve to fill the gap between the highly detailed (but far more slowly acquired) cellular images obtained by confocal microscopy and the imageless but rapidly acquired data obtained by immunofluorescence-based multiparameter flow cytometry. The ImageStreamX combines these two later technologies, providing a synergistic advantage over each used in tandem. The requested instrument quantifies both the intensity and intracellular location of fluorescent cellular probes and can image at high rates of speed (up to ~2000 cells/sec), allowing our consortium of Massachusetts General Hospital (MGH) investigators to analyze rare sub- populations and highly heterogeneous samples with statistically robust and objective results. This technology will permit some investigations that would otherwise be impossible to perform, to actually be implemented. By combining the speed, sensitivity, and immunophenotyping abilities and capacities of flow cytometry with the detailed imagery and functional insights of microscopy, the requested instrument will be an invaluable addition to represented researchers at both downtown Boston and Charlestown research campuses of MGH, and throughout the greater Partners Health Care System. The instrument utilizes the same dyes and markers employed in fluorescence microscopy and flow cytometry and can perform virtually any standard flow cytometry assay with the added value of quantitative visual inspection and resulting confirmation. The requested instrument will enable 30 investigators representing 77 NIH funded grants to enter into entirely new and useful areas of investigation. There is currently no similar instrumentation at MGH, anywhere in Partners Health Care/Harvard University, or the surrounding geographic region. A wide variety of studies related to cell biology, immunology, autoimmune, infectious disease, oncology and stem cell research will benefit from the placement of this instrumentation in the principal investigator's laboratory. An ImageStreamX demo-unit was placed in the principal investigator's laboratory in February 2010 for one week; pilot data produced from those 5-days is presented in this submission as well as updated data for this 2011 re-submission. The co-investigators in this application represent over 27 million dollars of NIH funded work based at MGH; the entire hospital of approximately 1000 principal investigator's has a NIH budget of approximately $325 million dollars.

描述（由申请人提供）：在此重新提交中要求NIH NIH NCRR资金购买5型激光板2摄像机AMNIS Imagestreamx，其中三个（20倍，40倍，60x）目标镜头。这个新平台是大约十一年前推出的原始仪器AMNIS的一个大大改进，精致且功能强大的版本。该机构支持和专业监督的工具将放置在主要研究者现有病理学系流式细胞仪核心，该核心核心已经成功运营了将近30年。所请求的仪器将用于填补通过共聚焦显微镜获得的高度详细（但更缓慢获取的）细胞图像与通过基于免疫荧光的多参数流式细胞仪获得的无图像但迅速获取的数据之间的差距。 ImageStreamx结合了这两种后来的技术，比串联使用的每种技术都提供了协同的优势。所请求的仪器量化了荧光细胞探针的强度和细胞内位置，并且可以以高速速度（最高〜200​​0个单元/秒）进行成像，从而使我们的马萨诸塞州一般医院（MGH）研究人员分析了稀有的亚种群和具有统计稳健性和目标结果的高度异构样品。这项技术将允许进行一些否则无法执行的调查，实际上实施。通过将流式细胞仪的速度，灵敏度和免疫表型能力以及显微镜的详细图像和功能见解相结合，所需的仪器将是波士顿市中心和MGH市中心的研究人员的宝贵补充，这将是MGH和Charlestown研究校园的代表研究人员，以及整个更大的Partners Chare Care Care System。该仪器利用在荧光显微镜和流式细胞仪中采用的相同染料和标记物，几乎可以执行任何标准的流式细胞仪测定法，并具有定量视觉检查的附加值和结果确认。所请求的工具将使30名代表77名NIH资助的赠款的调查人员能够进入全新且有用的调查领域。目前在MGH，合作伙伴医疗保健/哈佛大学或周围地理区域的任何地方都没有类似的仪器。与细胞生物学，免疫学，自身免疫性，传染病，肿瘤学和干细胞研究有关的各种研究将受益于该仪器在首席研究者的实验室中的放置。 Imagestreamx演示单元于2010年2月在首席研究人员的实验室中放置了一周；此提交中介绍了从这些五日制作的试点数据，以及2011年重新提交的更新数据。本申请中的共同投资者代表了MGH的2700万美元的NIH资助工作；大约1000名主要研究人员的整个医院的NIH预算约为3.25亿美元。