Modeling of Hantavirus Reservoirs in Brazil

巴西汉坦病毒库建模

• 批准号: 7559564
• 负责人: Colleen B Jonsson
• 金额: $ 3.47万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-20 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7559564
• 关键词: Affect; Antibodies; Applications Grants; Area; Biodiversity; Biological Assay; Blood; Brazil; Cells; Clinical; Data; Databases; Diagnosis; Disease; Ecology; Ecosystem; Environment; Grant; Habitats; Hantavirus; Hantavirus Infections; Health; Human; Incidence; Individual; Infection; Information Systems; Investigation; Latin America; Life; Location; Longitudinal Studies; Maps; Methods; Modeling; Monitor; Movement; Neutralization Tests; Paraguay; Parents; Patients; Phylogenetic Analysis; Physicians; Population; Prevalence; Procedures; Property; Public Health; Reporting; Reproduction; Reproductive History; Research; Research Personnel; Research Training; Rodent; Sampling; Scientist; Series; Site; Specimen; Technology Transfer; Testing; Time; Training; Virus; Visit; Work; anthropogenesis; base; biosafety level 3 facility; experience; forest; human disease; land cover; multidisciplinary; transmission process; viral RNA

DESCRIPTION (provided by applicant): The parent R01 grant for this proposal, "Land Cover Change in the Chaco and Interior Atlantic Forest (IAF) in Paraguay on Hantavirus Ecology " is testing the hypothesis that the rapid anthropogenic land cover change occurring in Paraguay will have a direct impact of ecosystem viability and biodiversity, and therefore, will affect rodent-hantavirus ecology. From 2003 to present, Brazil has reported 670 cases. In the Brazilian Department, where we propose the research to take place, there were 40 cases (Pincelli et al., 2003). Extension of this project to Brazil will build several important capacities. They include: 1. Approaches and methods in geospatial model building. 2. Approaches and methods in capturing rodents and taking samples from rodents which may harbor possible serious human diseases. 3. Approaches and methods toward general daily work with Hantavirus a BSL3 and isolation of viruses from the Brazilian samples. 4. Training and research opportunities for. Paraguayan scientists at the BSL3 facility in Riberao Preto. The specific aims of the research and capacity building include: Aim 1: To characterize the temporal dynamics of rodent population changes at selected sites in Brazil; Aim 2. To isolate Hantaviruses from the rodents collected in Aim 1 as well as to characterize their prevalence in rodents; Aim 3: To model the relationship between landscape and hantavirus occurrence in human cases in Brazil. Importantly, the objectives reached in aims 1 and 2 will permit analyses of the human infections in further depth and this information will be added to the modeling in Aim 3. Currently, in Latin America, the vast majority of the Hantaviruses that have been identified, have not been isolated. Hence, physicians and public health workers do not know to what virus their patient was exposed. In Latin America this is an important part of the clinical picture because of the presence of numerous strains of Hantavirus circulating in close or overlapping proximity. To truly understand the impact of the virus on human health, further diagnosis of the patient for the virus strain through neutralization assays will be valuable. As in the US, Brazil is building BSL3 facilities that can accommodate this work. Isolation of the viruses and the neutralization test requires working with live virus at BSL3. A major effort of this grant will be to transfer the technology to build the BSL3 capacity.

描述（由申请人提供）：该提案的父级R01赠款：“汉坦病毒生态学上的巴拉圭的Chaco和室内大西洋森林（IAF）的土地覆盖变化正在测试以下假设：帕拉圭发生的快速人为土地覆盖物在巴拉圭发生的快速覆盖物将对生态系统的Ecodys ecodiver and Bibortiver and-Hant and tall card rad rad carlir and wall card rad rad rad ravent，因此。从2003年到现在，巴西报告了670例。在我们建议进行研究的巴西部门中，有40例（Pincelli等，2003）。将该项目扩展到巴西将建立几个重要的能力。它们包括：1。地理空间模型构建中的方法和方法。 2。捕获啮齿动物并从啮齿动物中取样的方法和方法，这些啮齿动物可能带有可能严重的人类疾病。 3。与汉塔病毒A BSL3的一般日常工作的方法和方法，并从巴西样品中分离病毒。 4。培训和研究机会。 Riberao Preto的BSL3设施的巴拉圭科学家。研究和能力建设的具体目的包括：目标1：表征巴西选定地点啮齿动物种群变化的时间动态； AIM 2。将汉坦病毒与AIM 1收集的啮齿动物分离，并表征它们在啮齿动物中的患病率；目标3：在巴西人类病例中建模景观与汉坦病毒之间的关系。重要的是，目标1和2中达到的目标将允许进一​​步分析人类感染，并将这些信息添加到AIM 3中的建模中。目前，在拉丁美洲，尚未鉴于已鉴定出的绝大多数汉坦病毒。因此，医生和公共卫生工作者不知道患者暴露了哪些病毒。在拉丁美洲，这是临床图片的重要组成部分，因为存在许多在近距离接近或重叠的汉坦病毒菌株。为了真正了解病毒对人类健康的影响，通过中和分析进一步诊断患者病毒菌株将是有价值的。与美国一样，巴西正在建造可容纳这项工作的BSL3设施。病毒的分离和中和测试需要在BSL3处使用活病毒。这笔赠款的重大努力是转移技术以构建BSL3容量。