Core D-- Mitochoindria Core

核心D为该计划中的所有单个项目提供支持，并为 磷脂、酰基肉毒碱和丙二酰辅酶A的测量;线粒体和酶的分离 活性测量;制备组织标本和分离的线粒体用于形态学检查 分析. 具体目标是： 1.对照组和微栓塞组心肌肌膜下和原纤维间线粒体的分离 心力衰竭犬氧化磷酸化测定项目4。 2.测定电子传递链酶的活性。 3.提供硅胶柱层析类分离脂类及高效液相 磷脂类的色谱分析。 4.使用高效液相色谱法对酰基肉毒碱、丙二酰辅酶A和磷脂进行定量和定性分析。 高效液相色谱/电喷雾质谱 5.提供修饰蛋白质和/或肽的分子量和氨基酸序列测定 使用高效液相色谱/电喷雾电离质谱法。 6.提供额外的分析支持程序，即使核心拥有资源和专业知识， 尽管这些程序在特定项目中的范围有限。

Core D provides support to all individual projects in the program and provides methodology for the measurement of phospholipids, acylcarnitines and malonyl-CoA; isolation of mitochondria and enzyme activity measurements; preparation of tissue specimens and isolated mitochondria for morphological analysis. The specific aims are: 1. Isolation of heart subsarcolemmal and interfibrillar mitochondria from control and microembolizationinduced heart failure dogs for oxidative phosphorylation measurement by Project 4. 2. Determine the activity of the electron transport chain enzymes. 3. Provide silica gel column chromatographic class separation of lipids and high performance liquid chromatographic analysis of phospholipid classes. 4. Provide quantitative and qualitative analysis of acylcarnitines, malonyl-CoA and phospholipids using high performance liquid chromatography/electrospray mass spectrometry. 5. Provide molecular weight and amino acid sequence determinations of modified proteins and/or peptides using high performance liquid chromatography/electrospray ionization mass spectrometry. 6. Provide additional analytical support procedures that the Core has the resources and expertise in even though the procedures are of limited scope in a particular project.