Small Animal Bone Phenotyping Core

小动物骨表型核心

• 批准号: 7622628
• 负责人: TIMOTHY R NAGY
• 金额: $ 9.91万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7622628
• 关键词: Animal Housing; Animal Model; Animals; Area; Arts; Biology; Bone Diseases; Bone Mineral Contents; Cell Differentiation process; Cellular biology; Complement; Consultations; Core Facility; Data; Development; Development Plans; Diet; Dual-Energy X-Ray Absorptiometry; Education; Ensure; Experimental Designs; Feasibility Studies; Funding Mechanisms; Generations; Goals; Human Resources; Imaging Techniques; In Vitro; Individual; Interdisciplinary Study; Laboratories; Maintenance; Measurement; Measures; Metabolic Bone Diseases; Methods; Mission; Modeling; Operating System; Operative Surgical Procedures; Osteoporosis; Phenotype; Procedures; Publications; Quality Control; Research; Research Personnel; Resources; Rheumatoid Arthritis; Roentgen Rays; Role; Services; Techniques; Technology; Testing; Time; Validation; X-Ray Computed Tomography; bone; bone cell; bone imaging; bone loss; cost; density; gene therapy; in vivo; innovation; instrument; instrumentation; method development; novel; nutrition; quality assurance; ranpirnase; response; validation studies

The ability to rapidly screen animals for bone mineral content (BMC) and density (BMD) is of great importance. Recent advances in instrumentation, including dual-energy X-ray absorptiometry (DXA) and micro-computed tomography (uCT) have made it possible to determine BMC and BMD in vivo in small animal models. The proposed Small Animal Bone Phenotyping Core is a unique facility that has been developed by the Director, Dr. Timothy Nagy, to enable single projects to access state-of-the-art imaging techniques for the determination of BMC and BMD. The Core will provide all resources, including instrumentation and personnel, to carry out its mission. A centralized Core is required due to the cost of the instruments and the expertise required in their operations. The Director and personnel of the Core have demonstrated expertise in the effective use of this technology for the measurement of BMC and BMD in small animals, and are at the forefront of method development and validation of methods in this area. The Core will operate according to a formal quality assurance plan. In addition, the Core will provide extensive education in terms of the appropriate approaches, the limitations of the technology, and the interpretation of data. Extensive consultation on experimental design (especially animal housing and diet) also will be provided to ensure the generation of informative data. Two specific aims are proposed for this interdisciplinary Core: Specific Aim 1: To provided accurate and precise measures of bone mineral content and density in small animal models. This will be accomplished by providing a centralized core facility containing stateof- the-art instrumentation (DXA and uCT) for in vivo and ex vivo bone imaging. Specific Aim 2: Validate new techniques and procedures that will enhance research in bone biology and allow investigators to ask novel questions while at the same time being assured of the resultant data. The Core has been strategically developed in response to investigator demand and has in the past 4 years supported 20 projects instigated by 13 past or current CCBSR investigators, including four Pilot and Feasibility study investigators. It has already stimulated innovative studies in the areas of basic bone cell biology as well as interdisciplinary studies in the area of the development of gene therapy for bone diseases. It is anticipated that its role in the CCBSR will expand considerably over the next 5 years given the enhanced access provided by the P30 funding mechanism, and the increasing use of small animal models by the CCBSR investigators. The Core complements the efforts of the other two CCBSR research Cores and several investigators use all three Cores in their ongoing studies.

快速筛选动物骨矿物质含量（BMC）和密度（BMD）的能力是非常重要的。 重要性仪器的最新进展，包括双能X射线吸收法（DXA）和 微计算机断层扫描（uCT）已经使得在小的骨骼中测定体内BMC和BMD成为可能。 动物模型拟议的小动物骨表型核心是一个独特的设施， 由主任蒂莫西·纳吉博士开发，使单个项目能够访问最先进的成像 BMC和BMD测定技术。核心将提供所有资源，包括 仪器和人员，以执行其使命。由于以下成本，需要集中式核心 他们的业务所需的工具和专业知识。核心委员会主任和工作人员 在有效使用该技术测量BMC和BMD方面具有专业知识 在小动物中，并处于该领域方法开发和方法验证的最前沿。 核心将根据正式的质量保证计划运作。此外，核心将提供 在适当的方法、技术的局限性和 数据解释。对实验设计进行广泛咨询（尤其是动物饲养和饮食） 还将提供信息，以确保生成信息数据。为此提出了两个具体目标 跨学科核心： 具体目标1：提供准确和精确的骨矿物质含量和密度测量， 小动物模型这将通过提供一个集中的核心设施来实现，该设施包括： 用于体内和体外骨成像的最先进仪器（DXA和uCT）。 具体目标2：开发新技术和程序，以加强骨研究 生物学，并允许调查人员提出新的问题，同时确保 结果数据。 核心是根据研究者的需求进行战略性开发的，在过去4年中， 支持了由13名过去或现任CCBSR调查员发起的20个项目，包括4个试点项目和 可行性研究调查员。它已经刺激了基础骨细胞领域的创新研究， 生物学以及骨基因治疗发展领域的跨学科研究 疾病预计其在CCBSR中的作用将在未来5年内大幅扩大， P30供资机制提供的更多机会，以及越来越多地使用小动物 CCBSR研究人员的模型。核心补充了其他两个CCBSR研究的努力 核心和一些研究人员在他们正在进行的研究中使用所有三个核心。