Small Animal Bone Phenotyping Core

快速筛查动物的骨矿含量(BMC)和密度(BMD)的能力是非常重要的 重要性。仪器方面的最新进展，包括双能X射线吸收法(DXA)和 微型计算机断层扫描(UCT)使在小鼠体内测定BMC和BMD成为可能 动物模型。拟议的小动物骨分型核心是一个独特的设施，已经 由主任Timothy Nagy博士开发，使单个项目能够访问最先进的成像 骨矿含量和骨密度测定技术。核心将提供所有资源，包括 仪器和人员，以执行其使命。由于成本较高，需要一个集中的核心 他们的行动所需的仪器和专业知识。核心的主任和工作人员 在有效使用这项技术测量BMC和BMD方面表现出专业知识 在小动物身上，并走在这一领域方法开发和方法验证的前沿。 核心将根据正式的质量保证计划运行。此外，核心将提供 在适当的方法、技术的局限性和 数据的解释。就实验设计(特别是动物舍和饲料)进行广泛咨询 还将提供信息，以确保生成信息丰富的数据。为此提出了两个具体目标 跨学科核心： 具体目标1：提供准确和精确的骨矿含量和密度的测量 小动物模型。这将通过提供一个集中的核心设施来实现，该设施包含以下状态- 用于体内和体外骨骼成像的最先进的器械(DXA和UCT)。 具体目标2：验证将加强骨骼研究的新技术和新程序 生物学，并允许研究人员提出新的问题，同时确保 结果数据。 核心是根据调查人员的需求进行战略性开发的，并在过去4年中 支持由13名过去或现在的CCBSR调查人员发起的20个项目，包括4个试点和 可行性研究调查员。它已经刺激了基础骨细胞领域的创新研究 骨基因治疗发展领域中的生物学和跨学科研究 疾病。预计它在CCBSR中的作用将在未来5年内显著扩大。 P30筹资机制提供的更多机会，以及越来越多地使用小动物 CCBSR调查人员的模型。核心是对其他两项CCBSR研究工作的补充 Cores和几个研究人员在他们正在进行的研究中使用了所有这三个核心。

The ability to rapidly screen animals for bone mineral content (BMC) and density (BMD) is of great importance. Recent advances in instrumentation, including dual-energy X-ray absorptiometry (DXA) and micro-computed tomography (uCT) have made it possible to determine BMC and BMD in vivo in small animal models. The proposed Small Animal Bone Phenotyping Core is a unique facility that has been developed by the Director, Dr. Timothy Nagy, to enable single projects to access state-of-the-art imaging techniques for the determination of BMC and BMD. The Core will provide all resources, including instrumentation and personnel, to carry out its mission. A centralized Core is required due to the cost of the instruments and the expertise required in their operations. The Director and personnel of the Core have demonstrated expertise in the effective use of this technology for the measurement of BMC and BMD in small animals, and are at the forefront of method development and validation of methods in this area. The Core will operate according to a formal quality assurance plan. In addition, the Core will provide extensive education in terms of the appropriate approaches, the limitations of the technology, and the interpretation of data. Extensive consultation on experimental design (especially animal housing and diet) also will be provided to ensure the generation of informative data. Two specific aims are proposed for this interdisciplinary Core: Specific Aim 1: To provided accurate and precise measures of bone mineral content and density in small animal models. This will be accomplished by providing a centralized core facility containing stateof- the-art instrumentation (DXA and uCT) for in vivo and ex vivo bone imaging. Specific Aim 2: Validate new techniques and procedures that will enhance research in bone biology and allow investigators to ask novel questions while at the same time being assured of the resultant data. The Core has been strategically developed in response to investigator demand and has in the past 4 years supported 20 projects instigated by 13 past or current CCBSR investigators, including four Pilot and Feasibility study investigators. It has already stimulated innovative studies in the areas of basic bone cell biology as well as interdisciplinary studies in the area of the development of gene therapy for bone diseases. It is anticipated that its role in the CCBSR will expand considerably over the next 5 years given the enhanced access provided by the P30 funding mechanism, and the increasing use of small animal models by the CCBSR investigators. The Core complements the efforts of the other two CCBSR research Cores and several investigators use all three Cores in their ongoing studies.