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中文摘要
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流式细胞术是分析和分离最广泛使用和不可或缺的技术之一。 特定的细胞群。 CFAR 流式细胞术核心成立于 2003 年,拥有初始种子资金 来自 CFAR 赠款,为 CFAR 研究人员提供更好的先进流式细胞术使用机会 设备和技术。 CFAR 核心在 Einstein 的流式细胞仪资源中是独一无二的 提供生物危害遏制,以对需要生物安全 2 级的样品进行分析和分类 或更高的预防措施,因此是涉及艾滋病毒和各种与艾滋病相关的工作的主要资源 机会致病菌。 CFAR 流式细胞术核心为 CFAR 研究人员提供了随时访问 各种先进的分析流式细胞仪和细胞分选仪器以及广泛的技术 已建立的流式细胞术技术的性能和实施的专业知识和帮助 该领域的新技术。自成立以来的四年里,Core 取得了长足的发展 空间、设备和人员方面。这体现了这一核心设施所发挥的重要作用 在CFAR相关的研究项目中,以及强调价值的大量机构支持 核心的附加功能。核心的活动根据以下四个具体内容进行组织 目标: 目标 1. 维持流式细胞术研究所需的必要研究基础设施, 适当的生物安全遏制保障措施。 目标 2. 通过定期更新现有的流式细胞术资源和 及时引进新设备、新工艺。 目标 3. 为人们提供方便且经济高效的设备和技术专业知识 流式细胞术的性能和解释。 目标 4. 提供教育和培训,协助 CFAR 调查人员实施新的和 建立了流式细胞术技术。 相关性:流式细胞术使研究人员能够研究细胞或微生物的许多特性, 快速收集对于了解动物模型和人类疾病过程至关重要的信息 科目。 CFAR 流式细胞术核心支持此类所需的仪器和人员 的基础研究,并将这些资源组织在一个集中的设施中,为大型研究提供访问 许多研究人员正在研究与艾滋病毒感染和艾滋病相关的各种问题。
英文摘要
Flow cytometry is one of the most widely used and indispensable techniques for the analysis and isolation of specific cell populations. The CFAR Flow Cytometry Core was established in 2003 with initial seed funds from the CFAR grant to provide CFAR investigators with improved access to advanced flow cytometry equipment and techniques. The CFAR Core is unique among the flow cytometry resources at Einstein in providing biohazard containment for carrying out analysis and sorting of samples requiring Biosafety Level-2 or higher precautions, and is thus a major resource for work involving HIV and a variety of AIDS-related opportunistic pathogens. The CFAR Flow Cytometry Core provides CFAR investigators with ready access to a variety of advanced instruments for analytical flow cytometry and cell sorting, and extensive technical expertise and assistance for performance of established flow cytometry techniques and implementation of new technologies in this area. In the four years since its inception, the Core has grown substantially with regard to space, equipment and personnel. This reflects the important role that this core facility has played in CFAR related research projects, and also the considerable institutional support that underscores the value added features of the Core. The activities of the Core are organized according to the following four specific aims: Aim 1. To maintain the necessary research infrastructure needed for flow cytometry studies, with appropriate biosafety containment safeguards. Aim 2. To maintain cutting edge capabilities by regularly updating existing flow cytometry resources and introducing new equipment and techniques in a timely manner. Aim 3. To provide convenient and cost-effective access to equipment and technical expertise for the performance and interpretation of flow cytometry. Aim 4. To provide education and training to assist CFAR investigators in the implementation of new and established flow cytometry techniques. RELEVANCE: Flow cytometry allows researchers to study many properties of cells or micro-organisms and rapidly gather information that is crucial to understanding disease processes in animal models and human subjects. The CFAR Flow Cytometry Core supports instruments and personnel that are required for this type of essential research, and organizes these resources in a centralized facilty that provides access for a large number of investigators working on a wide range of problems relevant to HIV infection and AIDS.
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