Flowcytometry Core

流式细胞术是一种应用最广泛、不可或缺的分析和分离技术。 特定的细胞群体。CFAR流式细胞仪核心成立于2003年，初始种子资金 来自CFAR的拨款，为CFAR调查人员提供更好的先进流式细胞术 设备和技术。CFAR核心在爱因斯坦的流式细胞仪资源中是唯一的 为要求生物安全等级-2的样品进行分析和分类提供生物危害遏制 或更高的预防措施，因此是涉及艾滋病毒和各种与艾滋病有关的工作的主要来源 机会主义的病原体。CFAR流式细胞术核心为CFAR调查人员提供了随时访问 各种先进的分析流式细胞仪和细胞分选仪器，以及广泛的技术 已建立的流式细胞仪技术的执行和实施方面的专门知识和援助 这一领域的新技术。在其成立以来的四年中，核心业务大幅增长， 在空间、设备和人员方面。这反映了这一核心设施所发挥的重要作用 在CFAR相关研究项目中，以及突显其价值的相当大的机构支持 增加了核心的功能。核心小组的活动是根据以下四个具体情况组织的 目标： 目标1.维持流式细胞术研究所需的必要研究基础设施， 适当的生物安全遏制保障措施。 目标2.通过定期更新现有的流式细胞仪资源和 及时引进新设备、新技术。 目标3.提供方便和具有成本效益的获取设备和技术专门知识的途径 流式细胞术的表现和解释。 目标4.提供教育和培训，协助CFAR调查员执行新的和 建立了流式细胞术技术。 相关性：流式细胞术使研究人员能够研究细胞或微生物的许多特性，并 快速收集对了解动物模型和人类疾病过程至关重要的信息 研究对象。CFAR流式细胞仪核心支持这种类型所需的仪器和人员 并将这些资源组织在一个集中化的设施中，该设施为大型 许多调查人员致力于与艾滋病毒感染和艾滋病有关的广泛问题。

Flow cytometry is one of the most widely used and indispensable techniques for the analysis and isolation of specific cell populations. The CFAR Flow Cytometry Core was established in 2003 with initial seed funds from the CFAR grant to provide CFAR investigators with improved access to advanced flow cytometry equipment and techniques. The CFAR Core is unique among the flow cytometry resources at Einstein in providing biohazard containment for carrying out analysis and sorting of samples requiring Biosafety Level-2 or higher precautions, and is thus a major resource for work involving HIV and a variety of AIDS-related opportunistic pathogens. The CFAR Flow Cytometry Core provides CFAR investigators with ready access to a variety of advanced instruments for analytical flow cytometry and cell sorting, and extensive technical expertise and assistance for performance of established flow cytometry techniques and implementation of new technologies in this area. In the four years since its inception, the Core has grown substantially with regard to space, equipment and personnel. This reflects the important role that this core facility has played in CFAR related research projects, and also the considerable institutional support that underscores the value added features of the Core. The activities of the Core are organized according to the following four specific aims: Aim 1. To maintain the necessary research infrastructure needed for flow cytometry studies, with appropriate biosafety containment safeguards. Aim 2. To maintain cutting edge capabilities by regularly updating existing flow cytometry resources and introducing new equipment and techniques in a timely manner. Aim 3. To provide convenient and cost-effective access to equipment and technical expertise for the performance and interpretation of flow cytometry. Aim 4. To provide education and training to assist CFAR investigators in the implementation of new and established flow cytometry techniques. RELEVANCE: Flow cytometry allows researchers to study many properties of cells or micro-organisms and rapidly gather information that is crucial to understanding disease processes in animal models and human subjects. The CFAR Flow Cytometry Core supports instruments and personnel that are required for this type of essential research, and organizes these resources in a centralized facilty that provides access for a large number of investigators working on a wide range of problems relevant to HIV infection and AIDS.