The Primate Embryo Gene Expression Resource

灵长类动物胚胎基因表达资源

• 批准号: 7590133
• 负责人: Keith E Latham
• 金额: $ 30.26万
• 依托单位: TEMPLE UNIV OF THE COMMONWEALTH
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至 2008-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7590133
• 关键词: Animal Husbandry; Animal Model; Animals; Area; Basic Science; Biological Markers; Biological Models; Cattle; Cell Therapy; Cells; Clinical; Communities; Compatible; Data; Databases; Development; Disease; Dot Immunoblotting; Embryo; Embryology; Embryonic Development; Emerging Technologies; Evaluation; Event; Fertilization; Fertilization in Vitro; Funding; Gene Expression; Genes; Health; Hormonal; Human; In Vitro; Inner Cell Mass; Knowledge; Libraries; Macaca mulatta; Messenger RNA; Methods; Modeling; Molecular; Molecular Biology; Mus; National Center for Research Resources; Numbers; Oocytes; Operative Surgical Procedures; Oryctolagus cuniculus; Papio; Pattern; Population; Preimplantation Diagnosis; Primates; Procedures; Production; Proteins; Protocols documentation; Purpose; Rate; Reproduction; Reproductive Medicine; Research; Research Personnel; Resources; Sampling; Scientist; Screening procedure; Staging; Stem cells; System; Techniques; Testing; base; blastocyst; cDNA Library; clinical effect; cost; gene discovery; gene function; hatching; in vivo; interest; mammalian embryology; mature animal; natural Blastocyst Implantation; nonhuman primate; novel; oocyte maturation; programs; research study; stem cell therapy; subtraction hybridization; success; tool

The Primate Embryo Gene Expression Resource permits the rapid and cost-effective study of gene expression in primate oocytes and preimplantation embryos using the rhesus monkey as the primary model organism. PREGER contains over 160 informative samples representing GV-stage oocytes, MII- stage oocytes, and embryos at the 1-cell through hatched blastocyst stages, obtained using different combinations of in vitro or in vivo maturation, fertilization, and culture. This extensive and valuable sample set permits analyses of gene expression related to understanding basic primate embryology, as well as potential effects of clinical procedures on embryo quality using a primate model. Dot blots for expression analysis, cDNA libraries, probes, and other tools in PREGER can be distributed to other scientists, providing unprecedented opportunities for molecular studies in primate embryos. The libraries can be employed for a myriad of other purposes, such as library screening and library subtraction. We propose to maintain the resource, continue to distribute these materials and molecular tools to the scientific community, maintain the PREGER website, extend the resource to encompass samples from baboon in order that the generality of observations among primate species may be evaluated, and incorporate samples of human and rhesus ES lines and rhesus inner cell mass cells for evaluating the developmental potency of human ES lines. We also we propose to apply a novel method for suppression subtraction hybridization that we have developed to be compatible with our amplified libraries, in order to identify novel developmentally regulated genes that are relevant to early primate embryogenesis and that will assist improvements in assisted reproduction methods. This will facilitate basic research, and may support novel clinical approaches, e.g., in the evaluation of embryo quality. The proposed activities will at once maintain and expand the resource, produce new and fundamental basic information about primate embryos, and produce novel molecular approaches that may be useful in clinical practice.

灵长类胚胎基因表达资源允许快速和成本效益的基因研究 在灵长类卵母细胞和植入前胚胎中的表达， 模式生物PREGER包含超过160个代表GV期卵母细胞、MII- 阶段的卵母细胞和1-细胞至孵化胚泡阶段的胚胎，使用不同的方法获得 体外或体内成熟、受精和培养的组合。这种广泛而有价值的 样本集允许分析与理解基本灵长类胚胎学相关的基因表达， 以及使用灵长类动物模型研究临床程序对胚胎质量的潜在影响。斑点印迹法 PREGER中的表达分析、cDNA文库、探针和其他工具可以分发给其他人， 这为灵长类动物胚胎的分子研究提供了前所未有的机会。图书馆 可用于无数其它目的，例如文库筛选和文库消减。我们 我建议维持资源，继续将这些材料和分子工具分发给 科学界，维护PREGER网站，扩大资源，包括样本， 狒狒，以便评估灵长类物种中观察结果的一般性，以及 掺入人和恒河猴ES系和恒河猴内细胞团细胞的样品，用于评估 人ES系的发育潜能。我们还提出了一种新的抑制方法， 我们已经开发了与我们的扩增文库兼容的消减杂交，以便 鉴定与早期灵长类动物胚胎发生相关的新的发育调控基因， 将有助于辅助生殖方法的改进。这将促进基础研究，并可能 支持新的临床方法，例如，评估胚胎质量。拟议的活动将 立即维护和扩大资源，产生关于灵长类动物的新的和基本的基本信息 胚胎，并产生可能在临床实践中有用的新的分子方法。