Low cost portable flow cytometry (R21/R33)

所提供的空间。 该项目的目标是开发用于生物医学应用的低成本便携式流式细胞仪。 这类工具将在包括医学(艾滋病毒和癌症诊断)在内的许多领域产生重大影响， 国土防务(生物点探测、生物监视和法医分析)和一般 生物医学研究(配体-受体研究、分子组装分析、高通量筛选和 基因分型)。许多强大的应用程序使流式细胞仪成为几乎每所大学的固定设备， 医学院、制药公司和诊断实验室。然而，规模、费用和要求 因为大量的消耗品仅限于在正规的实验室环境中使用。一款低成本的便携式电脑 流式细胞仪将使所有临床医生和研究人员都可以使用这些应用程序，这将具有 对世界卫生和研究产生重大影响。 为了开发这样的仪器，我们将(在R21阶段)开发允许粒子聚焦的技术 不使用鞘液。鞘液体的清除将导致增加可携带性 仪器，还减少了消耗品的需求。我们的新技术将允许一种无鞘便携式 流式细胞仪可以达到常规的颗粒分析率，同时使用的功率和 传统的流式细胞仪的耗材。我们还将开发低功率激励/检测系统 (R21相)，不需要广泛的温度调节即可实现光学测量稳定性。是这样的 这将显著降低流式细胞仪的尺寸、成本和功率要求。 最后，我们将把上述技术集成到一个微型的、负担得起的流式细胞仪中，具有先进的 样品处理能力显著降低了消耗品和电力需求(R33 阶段)。为了做到这一点，我们将把上述技术结合到微型制造的流式细胞仪核心中 可轻松连接样品处理和光学模块。我们还将开发一种微型Low 电力数据采集系统，使之能够组装成完整的微型低功率流式细胞仪。我们 将使用定量流式细胞术技术验证仪器性能。

SPACE PROVIDED. The goal of this project is to develop low cost portable flow cytometers for use in biomedical applications. Such instruments would have a large impact in many areas including medicine (HIV and cancer diagnosis), homeland defense (biological point detection, bio-surveillance, and forensic analysis) and general biomedical research (ligand-receptor studies, molecular assembly analysis, high throughput screening and genotyping). The many powerful applications have made flow cytometers a fixture in nearly every university, medical school, pharmaceutical company, and diagnostic lab. However, the size, expense and requirement for large amounts of consumables have limited their use to formal laboratory settings. A low cost portable flow cytometer would make these applications available to all clinicians and researchers, which would have a great impact on world health and research. To develop such an instrument we will develop (in the R21 phase) technologies that allow particle focusing without the use of sheath fluid. The elimination of sheath fluid will result in increased portability of the instrument and also reduce consumable requirements. Our novel technology will allow a sheathless portable flow cytometer to achieve conventional particle analysis rates, while using a fraction of the power and consumables of a conventional flow cytometer. We will also develop low power excitation/detection systems (R21 phase) that will not require extensive thermoregulation to achieve optical measurement stability. Such systems, will significantly reduce the size, cost and power requirements of a flow cytometer. Finally, we will integrate the above technologies into a miniature affordable flow cytometer, with advanced sample handling capabilities that has significantly reduced consumable and power requirements (R33 phase). To do this we will combine the above technologies into a microfabricated flow cytometer core that can easily be interfaced with sample handling and optical modules. We will also develop a miniature low power data acquisition system to enable assembly of a complete miniature low power flow cytometer. We will validate instrument performance using quantitative flow cytometry techniques.