Film Bulk Acoustic Resonator-based Ultra-Sensitive Biosensor Array Using Low Cost Piezoelectric Polymer as the Active Material

使用低成本压电聚合物作为活性材料的基于薄膜体声谐振器的超灵敏生物传感器阵列

• 批准号: EP/F06294X/1
• 负责人: Jack Luo
• 金额: $ 35.88万
• 依托单位: University of Bolton
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2008
• 资助国家: 英国
• 起止时间: 2008 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FF06294X%2F1
• 关键词: Film; Bulk; Acoustic; Resonator; based

Biosensors are a type of microdevices that are able to measure very small concentration of biological molecules or chemical substances through specific bio-binding or chemical absorption. Biosensors are extremely useful in diagnosis, fighting terrorist and prevention of pandemic disease spread. Through detection of associated molecules such as DNA and antibody-antigen, they are very promising in early diagnosis of cancers and genetic disorder. Widespread applications of thus biosensors will lead to fast and accurate diagnosis, thus preventing unnecessary mortality and saving thousands of lives. Deployment of biosensors at key public locations enables detection of disease or biological substances in time, preventing spread of diseases or biochemical attach. High quality biosensors must be very sensitive, easy to use, low cost and fast with integrated electronics. Also multi-detection of many molecules using arrays is essential for reliable diagnosis and detection. Although many technologies have been developed such as microarrays and label-free electrochemical and optical biosensors. they have various shortages: lack of sensitivity and resolution, bulky and precise control of the sample position, or a large device size and lack of scalability etc. A multi-disciplinary team from Universities of Cambridge (CU), University of Manchester (MU) and University of Bolton (BU) is formed to develop a technology platform for biochemical detection using the most advanced film bulk acoustic wave resonator (FBAR) technology. FBAR device has a structure similar to quartz crystal microbalance but with a submicrometer thick piezoelectric (PE) active layer. It consists of a thin PE-layer with electrodes on both sides. Application of A.C. signals generates a standing wave between the two electrodes through PE effect. The resonant frequency is extremely sensitive to mass attached on the electrode surface owing to small device dimensions (thus the small base mass) and high operating frequency. Extremely small concentration of biomolecules can be detected through specific bio-binding with pre-deposited probe molecules on the electrode surface. The device has the combined merits of all other biosensors: label-free, ultra-high sensitivity and low detection limit, small dimensions, suitability for multi-detection using FBAR arrays, electronic output signal and low cost. The project will initially focus on development of high performance FBARs using piezoelectric (PE) ZnO thin films owing to its relatively mature technology. Biosensing technology will be developed in parallel using prostate-specific antigens (PSA) and peptide aptamers that specifically bind to those PSAs. Peptide aptamers have much better stability and specificity than proteins. Development of ZnO-based FBAR biosensors enables us to clarify all issues in device modelling, fabrication and characterisation, immobilization and biodetection etc. At the second stage, the project will develop novel FBARs on glass and plastic substrates using low cost PE-polymers. PE polymers such as polyvinylidene fluoride (PVDF) and its copolymer PVDF/TrFE have a piezoelectric constant and coupling coefficient comparable to the piezoelectric ceramics, and are biocompatible and chemically inert. Owing to their flexibility, it allows fabrication on low cost glass and plastic substrates. The cost of these biosensors will be extremely low. BU has excellent facilities for modelling and design, and for material and device characterisation. They will be responsible for modelling, design and characterisation. CU has a world-class cleanroom housed with excellent deposition, etch and microfabrication facilities. They will offer the expertise and experiences in device fabrication. The MU has first class biolab environment and relevant facilities for biological research. They are experts in protein adsorption, interfacial conformation, structural unfolding, and synthesis and cloning of peptide aptamers.

生物传感器是一种能够通过特定的生物结合或化学吸收来测量非常小浓度的生物分子或化学物质的微型器件。生物传感器在诊断、打击恐怖分子和预防流行病传播方面非常有用。通过检测DNA、抗体-抗原等相关分子，在癌症和遗传病的早期诊断中具有广阔的应用前景。因此，生物传感器的广泛应用将导致快速和准确的诊断，从而防止不必要的死亡和挽救成千上万的生命。在主要公共场所部署生物传感器，可以及时检测疾病或生物物质，防止疾病传播或生化附着。高质量的生物传感器必须非常灵敏，易于使用，成本低，集成电子器件快速。此外，使用阵列的许多分子的多重检测对于可靠的诊断和检测是必不可少的。尽管已经开发了许多技术，例如微阵列和无标记电化学和光学生物传感器。他们有各种各样的缺点：针对目前生物化学检测技术中存在的一些问题，如灵敏度和分辨率不够、样品位置控制笨重和精确度不够、设备尺寸过大和缺乏可扩展性等，由剑桥大学（CU）、曼彻斯特大学（MU）和博尔顿大学（BU）组成的多学科团队，利用最先进的薄膜体声波谐振器（FBAR）技术，开发了一个用于生物化学检测的技术平台。FBAR器件具有类似于石英晶体微天平的结构，但具有亚微米厚的压电（PE）活性层。它由一个薄的PE层和两侧的电极组成。应用A.C.信号通过PE效应在两个电极之间产生驻波。由于小的器件尺寸（因此小的基本质量）和高的工作频率，谐振频率对附着在电极表面上的质量极其敏感。通过与预先沉积在电极表面上的探针分子的特异性生物结合，可以检测极低浓度的生物分子。该器件具有所有其他生物传感器的综合优点：无标记，超高灵敏度和低检测限，尺寸小，适用于使用FBAR阵列的多检测，电子输出信号和低成本。由于技术相对成熟，该项目最初将重点开发使用压电（PE）ZnO薄膜的高性能FBAR。生物传感技术将使用前列腺特异性抗原（PSA）和特异性结合这些PSA的肽适体平行开发。肽适体具有比蛋白质好得多的稳定性和特异性。ZnO基FBAR生物传感器的发展使我们能够澄清在设备建模，制造和表征，固定和生物检测等所有问题在第二阶段，该项目将开发新型FBAR的玻璃和塑料基板上使用低成本的PE聚合物。PE聚合物如聚偏二氟乙烯（PVDF）及其共聚物PVDF/TrFE具有与压电陶瓷相当的压电常数和耦合系数，并且具有生物相容性和化学惰性。由于其灵活性，它允许在低成本的玻璃和塑料基板上制造。这些生物传感器的成本将非常低。BU拥有出色的建模和设计设施，以及材料和器件特性。他们将负责建模、设计和特性描述。CU拥有世界一流的洁净室，拥有出色的沉积，蚀刻和微加工设施。他们将提供设备制造方面的专业知识和经验。该大学拥有一流的生物实验室环境和生物研究的相关设施。他们是蛋白质吸附、界面构象、结构展开以及肽适体合成和克隆方面的专家。

项目成果

High Performance Shape Memory Polyurethane Synthesized with High Molecular Weight Polyol as the Soft Segment

• DOI: 10.3390/app2020535
• 发表时间: 2012-06-01
• 期刊: APPLIED SCIENCES-BASEL
• 影响因子: 2.7
• 作者: Ahmad, Manzoor;Xu, Bin;Luo, Jikui
• 通讯作者: Luo, Jikui

Chemically Sensitized Thin-Film Bulk Acoustic Wave Resonators as Humidity Sensors

• DOI: 10.1149/1.3496003
• 发表时间: 2010-01-01
• 期刊: JOURNAL OF THE ELECTROCHEMICAL SOCIETY
• 影响因子: 3.9
• 作者: Ashley, G. M.;Kirby, P. B.;Luo, J. K.
• 通讯作者: Luo, J. K.