Genotyping, Sequencing and Gene Expression

基因分型、测序和基因表达

• 批准号: 7786009
• 负责人: QING WANG
• 金额: $ 42.53万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7786009
• 关键词: Genotyping; Sequencing; Gene; Expression

The Sequencing, Genotyping and Gene Expression Core will act as a centralized facility to perform large scale and high throughput sequencing, genotyping and microarray analysis for the four projects. Specifically, the Core activities will include: 1) DNA sequence analysis; 2) identification of single nucleotide polymorphisms (SNPs); 3) genotyping of SNPs; and 4) microarray analysis. The advantages for such a centralized facility include: 1) It conserves resources, and is more time and cost efficient; 2) It avoids unnecessary duplication of effort: 3) It standardizes quality control; and 4) It allows for inter-project comparison of results. In addition, the Core will also function as an educational resource to train members of the Program Project laboratories in proper sequencing, genotyping and gene expression methodologies and to provide advice for Project Leaders in the design and implementation of relevant experiments. The training will be valuable for Postdoctoral Fellows and Graduate Students and useful if the demands on the Core exceed its capacity at a particular time. The Sequencing, Genotyping and Gene Expression Core will be housed in the Center for Cardiovascular Genetics in ND40 of the Lemer Research Institute.

测序，基因分型和基因表达核心将作为一个集中的设施，为四个项目进行大规模和高通量测序，基因分型和微阵列分析。具体而言，核心活动将包括：1)DNA序列分析；2)单核苷酸多态性（snp）鉴定；3) snp基因分型；4)芯片分析。这种集中式设施的优点包括：1)节省资源，更具时间和成本效益；2)避免不必要的重复劳动；3)规范质量控制；4)它允许项目间的结果比较。此外，核心还将作为一个教育资源，培训项目实验室成员正确的测序、基因分型和基因表达方法