Genotyping, Sequencing and Gene Expression

基因分型、测序和基因表达

• 批准号: 6892790
• 负责人: QING WANG
• 金额: $ 40.09万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6892790
• 关键词: biomedical resource; gene expression; genotype; high throughput technology; microarray technology; nucleic acid sequence; single nucleotide polymorphism

The Sequencing, Genotyping and Gene Expression Core will act as a centralized facility to perform large scale and high throughput sequencing, genotyping and microarray analysis for the four projects. Specifically, the Core activities will include: 1) DNA sequence analysis; 2) identification of single nucleotide polymorphisms (SNPs); 3) genotyping of SNPs; and 4) microarray analysis. The advantages for such a centralized facility include: 1) It conserves resources, and is more time and cost efficient; 2) It avoids unnecessary duplication of effort: 3) It standardizes quality control; and 4) It allows for inter-project comparison of results. In addition, the Core will also function as an educational resource to train members of the Program Project laboratories in proper sequencing, genotyping and gene expression methodologies and to provide advice for Project Leaders in the design and implementation of relevant experiments. The training will be valuable for Postdoctoral Fellows and Graduate Students and useful if the demands on the Core exceed its capacity at a particular time. The Sequencing, Genotyping and Gene Expression Core will be housed in the Center for Cardiovascular Genetics in ND40 of the Lemer Research Institute.

测序、基因分型和基因表达中心将作为一个集中设施，为四个项目进行大规模和高通量测序、基因分型和微阵列分析。具体而言，核心活动将包括：1）DNA序列分析; 2）单核苷酸多态性（SNP）鉴定; 3）SNP基因分型; 4）微阵列分析。这种集中设施的优点包括：1）节省资源，更节省时间和成本; 2）避免不必要的重复工作; 3）加强质量控制; 4）允许项目间比较结果。此外，该核心还将作为一种教育资源，在正确的测序、基因分型和基因表达方法方面培训计划项目实验室的成员， 在相关实验的设计和实施方面为项目负责人提供建议。培训将是有价值的博士后研究员和研究生和有用的，如果对核心的需求超过其能力在特定的时间。测序、基因分型和基因表达核心将被安置在Lemer研究所ND 40的心血管遗传学中心。