GENOTYPING
基因分型
基本信息
- 批准号:7493853
- 负责人:
- 金额:$ 18.88万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2007
- 资助国家:美国
- 起止时间:2007-03-01 至 2011-02-28
- 项目状态:已结题
- 来源:
- 关键词:AllelesBiological AssayCloningDNADNA SequenceDNA Sequencing FacilityDataDetectionDiscriminationEvaluationGeneticGenetic PolymorphismGenotypeHydra PolypsIndividualLaboratoriesMethodologyPlasmidsPostdoctoral FellowProtocols documentationPublished DatabasePurposeQuality ControlReproducibilityResourcesRestRoboticsSNP genotypingSampling StudiesServicesStandardizationStandards of Weights and MeasuresStudentsSystemTestingTimeTrainingcDNA Probescost efficientdesigninterestmembernucleaseprogramsresearch study
项目摘要
The purpose of Core D is act as a centralized facility to perform large scale and high throughput genotyping
of genetic polymorphisms as well as routine DNA sequencing. The advantages for such a centralized facility
include: 1) Conservation of resources; the core is more time and cost efficient than individual labs; 2) Avoid
unnecessary duplication of effort: 3) Standardization of quality control; and 4) Facilitation of data sharing. In
addition, the Core will also function as an educational resource to train members of the Program Project
laboratories in proper genotyping methodologies and to provide advice for Project Leaders in the design and
implementation of relevant experiments. The training will be valuable for Postdoctoral Fellows and Graduate
Students and useful if the demands on the Core exceed its capacity at a particular time. The Genotyping
Core will provide one ABI Prism 7900HT Genetic Analyzer with SDS 384-well Block, and a semi-automatic
robotic system, Hydra II for high throughput SNP genotyping. We will use the 5' nuclease allelic
discrimination assay for large-scale, high throughput genotyping of SNPs. All SNP assays will be tested for
their accuracy and reproducibility. We have developed a strict protocol for evaluation of each SNP assay
where the SNP of interest is identified by the Wave system or direct sequencing. SNPs chosen from
published databases will be first confirmed by direct sequencing, followed by testing for the robustness and
reproducibility of the assay. If the SNP passes these tests, it will be genotyoed in the rest of study samples.
For further quality control, we include two negative controls (no DNA), two wells of allele 1 standard, and two
wells of allele 2 standard. The Genotyping Core represents an integral part of the Program Project. Projects
1 and 2 will use the SNP detection service extensively. The other projects will make use of the DNA
sequencing facility for analysis of plasmid probes and cDNA cloning (Project 3).
核心D的目的是作为执行大规模和高通量基因分型的集中式设施
遗传多态和常规DNA测序。这种集中式设施的优势
包括:1)节约资源;核心实验室比单独的实验室更省时、更具成本效益;2)避免
不必要的重复工作:3)质量控制标准化;4)促进数据共享。在……里面
此外,核心还将作为培训计划项目成员的教育资源
实验室采用适当的基因分型方法,并为项目负责人提供设计和
实施相关实验。该培训将对博士后研究员和研究生有价值
如果在某一特定时间对核心的需求超过其容量,则对学生很有用。基因分型
CORE将提供一台带有SDS384井区的ABI Prism 7900HT遗传分析仪,以及一台半自动
机器人系统,Hydra II,用于高通量SNP基因分型。我们将使用5‘核酸酶等位基因
用于大规模、高通量SNPs基因分型的鉴别试验。将对所有SNP检测进行检测
它们的准确性和重复性。我们已经制定了一套严格的方案来评估每一种SNP检测
其中感兴趣的SNP通过Wave系统或直接测序鉴定。SNPs选自
已发表的数据库将首先通过直接测序进行确认,然后进行稳健性和
测定的重现性。如果SNP通过了这些测试,它将在其余的研究样本中进行基因分型。
为了进一步的质量控制,我们包括两个阴性对照(没有DNA),两个等位基因1标准,以及两个
等位基因2的井标准。基因分型核心是方案项目的一个组成部分。项目
1和2将广泛使用SNP检测服务。其他项目将利用DNA
用于分析质粒探针和cdna克隆的测序设施(项目3)。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('QING WANG', 18)}}的其他基金
Quantitative Endogenous MRI Imaging of Neuroinflammation in AD
AD 神经炎症的定量内源 MRI 成像
- 批准号:
10334974 - 财政年份:2022
- 资助金额:
$ 18.88万 - 项目类别:
Quantitative Endogenous MRI Imaging of Neuroinflammation in AD
AD 神经炎症的定量内源 MRI 成像
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$ 18.88万 - 项目类别:
Characterization of the Neuroinflammation in Autosomal Dominant Alzheimer Disease Using Neuro-Inflammation Imaging
使用神经炎症成像表征常染色体显性阿尔茨海默病的神经炎症
- 批准号:
10196311 - 财政年份:2021
- 资助金额:
$ 18.88万 - 项目类别:
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