XRAY CRYSTALLOGRAPHY

描述(申请人提供)：X射线晶体学共享设施提供 为癌症中心成员和其他地区科学家提供独特的、最先进的结晶学设施。在过去的五年中，该设施获得了广泛的高通量能力，包括对Argonne Synchrotron设施的两条光束线的专用访问，一个全自动纳米结晶实验室，以及通过自动化微流控系统进行的液体扩散结晶。该设施还拥有四台旋转阳极发电机，带有多个图像板检测系统、结晶学计算和图形软件包，以支持结构研究。该设施目前正在支持20多个不同的癌症相关项目。该核心目前被用于支持主要癌症中心项目的结构研究，例如淋巴因子和细胞因子的结构研究，癌症中心孢子项目涉及“用于乳腺癌预防的新型维甲酸的临床前/临床开发”。该核心目前正被用于结晶新型维甲酸核受体复合体(RARs)，以支持预防皮肤癌以及治疗乳腺癌和白血病的药物开发。其他结构研究包括配体/DNA相互作用， 伴侣蛋白，载脂蛋白A-I，一种DNA修复内切酶，几种不同的Toll样蛋白 受体、Mark-3激酶与新型Pirn激酶抑制剂的开发。NCI的多名研究人员得出的结果突显了Core的新型结晶能力的价值。在过去的六个月里，核心能够产生五种不同的NCI蛋白质的晶体。

DESCRIPTION (provided by applicant): The X-ray Crystallography Shared Facility provides a unique, state-of-the art crystallography facility for Cancer Center members and other regional scientists. In the last five years, the facility has acquired extensive high through put capabilities, including dedicated access to two beamlines at the Argonne Synchrotron Facility, a fully-automated nano-crystallization laboratory and a liquid diffusion crystallization via an automated microfluidics system. The facility also has four rotating anode generators with multiple image plate detection systems, crystallographic computing and graphics packages to support structural studies. The facility is currently supporting more than twenty different cancer related projects. The Core is currently being used to support structural studies of major Cancer Center programs such as structural studies, of lymphokines and cytokines, the Cancer Center SPORE program involving "preclinical/clinical development of novel retinoids for breast cancer prevention". The Core is currently being used to crystallize novel retinoid-retinoic acid nuclear receptor complexes (RARs) to support drug development to prevent skin cancer and to treat breast cancer and leukemia. Other structural studies include ligand/DNA interactions, chaperone proteins, Apo-lipoprotein A-I, a DNA repair endonuclease, several different toll-like receptors, Mark-3 kinase and the development of novel pirn kinase inhibitors. The value of the Core's novel crystallization capabilities is highlighted by results produced from multiple investigators from the NCI. In the last six months the Core was able to produce crystals of five different NCI proteins.