MEDIA PREPARATION

描述(申请人提供)：UAB的媒体准备共享设施(MPSF) 综合癌症中心(UAB-CCC)是以下方面的集中资源：(A)真核细胞和细菌细胞培养液和补充剂；(B)常规细胞培养和分子生物学应用中使用的缓冲液和其他大容量试剂；(C)质量控制保证和测试；以及(D)关于细胞培养和分子生物学试剂的一般咨询和教育。总体目标是提高UAB-CCC研究活动的经济性、效率和质量。MPSF以低于现有商业价格的收费价格向UAB-CCC教职员工提供常见的培养介质、补充剂和缓冲液，从而提供经济实惠。这是通过(1)现场生产，无法产生额外的MPSF收入的退费率，以及(2)与商业供应商进行批量采购谈判，实现一般研究实验室无法获得的价格和运输费用减免来实现的。 MPSF通过方便的现场产品/服务可用性提供效率，与商业供应商相比，文书工作更少，交付速度更快。许多用户在首次请求后不到2小时内即可获得MPSF产品的所有权。MPSF通过以下方式提高质量：(A)对现场产品中的内毒素和无菌，以及研究人员提交的样本中的内毒素进行检测，以及现场高压灭菌器性能，以及(B)提供个性化服务，包括免费为特定实验问题提供专业咨询，以及以低成本设计和制备定制试剂。

DESCRIPTION (provided by applicant): The Media Preparation Shared Facility (MPSF) of the UAB Comprehensive Cancer Center (UAB-CCC) serves as a centralized resource for (a) eukaryotic and bacterial cell culture media and supplements, (b) buffers and other high-volume reagents used in routine cell culture and molecular biology applications, (c) quality control assurance and testing, and (d) general consultation and education with regard to cell culture and molecular biology reagents. The overall goals are to improve economy, efficiency and quality of UAB-CCC research activities. MPSF provides economy by supplying common culture media, supplements and buffers to UAB-CCC faculty at charge-back prices lower than available commercial prices. This is accomplished via (1) on-site production with charge-back rates that fail to generate excess MPSF income, and (2) volume purchase negotiations with commercial suppliers, yielding price and shipping charge reductions not available to the average research laboratory. MPSF offers efficiency by convenient on-site product/service availability, with less paperwork and faster delivery as compared to commercial suppliers. Many users take possession of MPSF products in less than 2 hours after initial request. MPSF promotes quality by (a) performing assays for endotoxin and sterility in on-site products, as well as for endotoxin in investigator-submitted samples, and on-site autoclave performance, and (b) offering personalized services including professional consultation for specific experimental problems at no cost, plus design and preparation of custom reagents at low cost.