Laser Speckle Imaging of Brain Tumor Vasculature

脑肿瘤脉管系统的激光散斑成像

• 批准号: 7536407
• 负责人: DAVID Lee SHERMAN
• 金额: $ 25.25万
• 依托单位: INFINITE BIOMEDICAL TECHNOLOGIES, LLC
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-24 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7536407
• 关键词: Algorithms; American; Animal Model; Area; Blood Vessels; Blood flow; Brain; Brain Neoplasms; Brain imaging; Cancer Patient; Cells; Central Nervous System Neoplasms; Cerebrum; Cessation of life; Clinical; Computer software; Contrast Media; Development; Diagnosis; Evaluation; Excision; Goals; Growth; Histology; Image; Imagery; Imaging Device; Imaging Techniques; Imaging technology; Immunohistochemistry; Implant; Individual; Invasive; Lasers; Lead; Life; Light; Magnetic Resonance Imaging; Malignant neoplasm of brain; Methodology; Methods; Modality; Modeling; Monitor; Nature; Neurological outcome; Operative Surgical Procedures; Pathology; Patients; Pharmaceutical Preparations; Pharmacologic Substance; Pharmacotherapy; Phase; Problem Solving; Public Health; Radiation therapy; Rattus; Research; Resolution; Resources; Rodent; Rodent Model; Site; Surface; System; Techniques; Technology; Testing; Therapeutic Intervention; Treatment Efficacy; Tumor Angiogenesis; Tumor-Associated Vasculature; Ultrasonography; United States; Week; angiogenesis; anticancer research; base; concept; density; gliosarcoma; image guided therapy; image processing; improved; innovation; instrumentation; novel; optical imaging; prototype; therapeutic target; tool; tumor; tumor growth; vector

DESCRIPTION (provided by applicant): Approximately 20,500 Americans will be diagnosed with a central nervous system tumor this year. Over half of those diagnosed will lose their lives. The mainstay of treatment for these patients has been surgical resection with radiation therapy. More recently, non-invasive pharmacotherapies have been developed to target inhibition of tumor angiogenesis. Evaluation of the vasculature during these treatment options is important and is aided by imaging modalities. However, the low resolution of ultrasound and cumbersome nature of MRI imaging present significant limitations. In addition, these modalities do not elucidate changes in tumor-associated microvasculature, which is important to investigate for angiogenesis-based research efforts. Thus, brain cancer research and therapeutic interventions would benefit from the development of imaging techniques to view high resolution, high contrast vascular images at the tumor site. We propose a new imaging modality based on the Laser Speckle (LS) phenomenon. LS optical imaging uses the random reflection nature of laser light to track the flow of blood at the vessel level. Unlike other modern imaging modalities, LS imaging does not require the use of a contrast agent and may be used at extremely high resolutions. We propose a Phase I effort focused on obtaining proof- of-concept for this imaging modality. Specifically, we will: " Develop an LS imaging system complete with an image processing suite to: 1) enhance the visualization of tumor-associated microvasculature, and 2) create wide field images to better visualize blood vessels across broader areas of the exposed brain. The microvasculature will be enhanced using a ridge tracking algorithm, and wide field images will be created by stitching together individual high-resolution images using a mosaicking method. " Validate the LS imaging technology in a rodent model of brain tumor by quantifying tumor vasculature using spatial calculations of Mean Vasculature Density and correlating with histological findings. The major milestone of Phase I is to produce high resolution and wide field images of tumor- associated vasculature with histological confirmation. If we are successful, we envision a Phase II effort focused on development of a LS imaging research prototype to track drug therapy and tumor angiogenesis in an animal model. The long-term goal is to build a clinical LS imaging device to improve visualization of surgical treatment in brain cancer patients. PUBLIC HEALTH RELEVANCE: The goal of this project is to develop a novel imaging modality based on laser speckle for evaluating brain tumor angiogenesis. This technology can be used to test new tumor drug therapies and eventually assist with intra-operative vasculature imaging during tumor resection.

描述（由申请人提供）：今年大约有20,500名美国人将被诊断出患有中枢神经系统肿瘤。超过一半的确诊患者将失去生命。这些患者的主要治疗方法是手术切除和放射治疗。最近，非侵入性药物治疗已经发展到针对肿瘤血管生成的抑制。在这些治疗方案中，血管系统的评估是重要的，并借助于成像方式。然而，超声的低分辨率和MRI成像的繁琐性质存在显着的局限性。此外，这些模式并不能阐明肿瘤相关微血管的变化，而这对于血管生成的研究是很重要的。因此，脑癌研究和治疗干预将受益于成像技术的发展，可以在肿瘤部位看到高分辨率、高对比度的血管图像。提出了一种基于激光散斑（LS）现象的成像方式。LS光学成像利用激光的随机反射特性来跟踪血管水平的血液流动。与其他现代成像方式不同，LS成像不需要使用造影剂，可以在极高的分辨率下使用。我们建议第一阶段的工作重点是获得这种成像模式的概念验证。具体来说，我们将：“开发一种LS成像系统，包括图像处理套件，以：1)增强肿瘤相关微血管的可视化，2)创建宽视场图像，以更好地显示暴露的大脑更广泛区域的血管。”微血管系统将通过脊跟踪算法得到增强，而宽视场图像将通过拼接方法将各个高分辨率图像拼接在一起。”通过使用平均血管密度的空间计算来量化肿瘤血管，并与组织学结果相关联，验证LS成像技术在啮齿动物脑肿瘤模型中的应用。第一阶段的主要里程碑是产生高分辨率和宽视野的肿瘤相关血管图像，并得到组织学证实。如果我们成功了，我们设想第二阶段的努力集中在LS成像研究原型的开发上，以跟踪动物模型中的药物治疗和肿瘤血管生成。长期目标是建立一种临床LS成像设备，以提高脑癌患者手术治疗的可视化。公共卫生相关性：该项目的目标是开发一种基于激光散斑的新型成像模式，用于评估脑肿瘤血管生成。该技术可用于测试新的肿瘤药物治疗，并最终协助肿瘤切除术过程中的术中血管成像。